Results 101 to 110 of about 1,236,846 (314)

Highly Improved Electrospray Ionization-Mass Spectrometry Detection of G-Quadruplex-Folded Oligonucleotides and Their Complexes with Small Molecules [PDF]

, 2017
G-quadruplexes are nucleic acids structures stabilized by physiological concentration of potassium ions. Because low stability G-quadruplexes are hardly detectable by mass spectrometry, we optimized solvent conditions: isopropanol in a triethylamine ...
Scalabrin, Matteo, Palumbo, Manlio, Richter, Sara N.   +2 more
core   +1 more source

LDAcoop: Integrating non‐linear population dynamics into the analysis of clonogenic growth in vitro

Molecular Oncology, EarlyView.
Limiting dilution assays (LDAs) quantify clonogenic growth by seeding serial dilutions of cells and scoring wells for colony formation. The fraction of negative wells is plotted against cells seeded and analyzed using the non‐linear modeling of LDAcoop.
Nikko Brix, Daniel Samaga, Katharina Gehr, Benedek Dankó, Mohamed Schumann, Guido Drexler, Ahmed Alnatsha, Georg Beyer, Ujjwal Mahajan, Martin Selmansberger, Julia Mayerle, Claus Belka, Horst Zitzelsberger, Kirsten Lauber   +13 more
wiley   +1 more source

High throughput screening for SARS-CoV-2 inhibitors targeting 5 helix bundle

SLAS Discovery
SARS-CoV-2 and other related viruses enter host cells via receptor recognition and membrane fusion. A crucial part of this is mediated by 5HB which is capable of binding to the viral spike heptad repeats (HR2) making 5HB a potential druggable target of ...
Emery Smith, Qibin Geng, Justin Shumate, Yuka Otsuka, Louis Scampavia, Thomas D. Bannister, Timothy P. Spicer   +6 more
doaj   +1 more source

Therapeutic strategies for MMAE‐resistant bladder cancer through DPP4 inhibition

Molecular Oncology, EarlyView.
We established monomethyl auristatin E (MMAE)‐resistant bladder cancer (BC) cell lines by exposure to progressively increasing concentrations of MMAE in vitro. RNA sequencing showed DPP4 expression was increased in MMAE‐resistant BC cells. Both si‐DPP4 and the DPP4 inhibitor sitagliptin suppressed the viability of MMAE‐resistant BC cells.
Gang Li, Shuichi Tatarano, Hirofumi Yoshino, Saeki Saito, Mitsuhiko Tominaga, Junya Arima, Ikumi Fukuda, Takashi Sakaguchi, Ryosuke Matsushita, Yasutoshi Yamada, Hideki Enokida   +10 more
wiley   +1 more source

Automating the application of smart materials for protein crystallization [PDF]

, 2015
The fabrication and validation of the first semi-liquid nonprotein nucleating agent to be administered automatically to crystallization trials is reported. This research builds upon prior demonstration of the suitability of molecularly imprinted polymers
Asanithi, Asherie, Caffrey, Chayen, Curcio, D'Arcy, D'Arcy, EL-Sharif, Frenkel, Georgiev, Gorrec, Hazim F. EL-Sharif, Kertis, Khurshid, Lata Govada, Liao, McPherson, Naomi E. Chayen, Newman, Nollert, Page, Rong, Sahir Khurshid, Saridakis, Saridakis, Shaw Stewart, Shim, Subrayal M. Reddy, Sugahara, Thakur, Zheng   +30 more
core   +2 more sources

PKA plays a conserved role in regulating gene expression and metabolic adaptation by phosphorylating Rpd3/HDAC1

Nature Communications
Cells need to reprogram their metabolism to adapt to extracellular nutrient changes. The yeast histone acetyltransferase SAGA (Spt-Ada-Gcn5-acetyltransferase) has been reported to acetylate its subunit Ada3 and form homo-dimers to enhance its ability to ...
Wenjing Dai, Qi Yu, Rui Ma, Zhu Zheng, Lingling Hong, Yuqing Qi, Fei He, Min Wang, Feng Ge, Xilan Yu, Shanshan Li   +10 more
doaj   +1 more source

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

High-Throughput Computational Screening of thermal conductivity, Debye temperature and Gr\"uneisen parameter using a quasi-harmonic Debye Model

, 2014
The quasi-harmonic Debye approximation has been implemented within the AFLOW and Materials Project frameworks for high-throughput computational science (Automatic Gibbs Library, AGL), in order to calculate thermal properties such as the Debye temperature
Asta, Mark, Curtarolo, Stefano, de Jong, Maarten, Levy, Ohad, Nardelli, Marco Buongiorno, Plata, Jose J., Toher, Cormac   +6 more
core   +1 more source

Rational methods for the selection of diverse screening compounds. [PDF]

, 2011
Traditionally a pursuit of large pharmaceutical companies, high-throughput screening assays are becoming increasingly common within academic and government laboratories.
Huggins, David J, Spring, David R, Venkitaraman, Ashok R   +2 more
core   +2 more sources

3D bioprinting for high-throughput screening: Drug screening, disease modeling, and precision medicine applications.

Applied Physics Reviews, 2019
High-throughput technologies have become essential in many fields of pharmaceutical and biological development and production. Such technologies were initially developed with compatibility with liquid handling-based cell culture techniques to produce ...
Andrea Mazzocchi, S. Soker, A. Skardal
semanticscholar   +1 more source