Results 301 to 310 of about 231,965 (320)
Some of the next articles are maybe not open access.
Complement C5a Receptor Assay for High Throughput Screening
Journal of Receptor Research, 1991The complement C5a receptor on U937 cells, a human histiocytic lymphoma cell line, stimulated with dibutyryl-cAMP have been stabilized for at least 3 months at a dilute, ready to use concentration. [125I]-Bolton Hunter labeled C5a, (recombinant, human) has been prepared by reverse phase HPLC to 2200 Ci/mmol. Using a filtration binding assay the Kd from
S R, Harris +4 more
openaire +2 more sources
High-Throughput Screening Assay for Biological Hydrogen Production
Analytical Chemistry, 2008This paper describes a screening assay, compatible with high-throughput bioprospecting or molecular biology methods, for assessing biological hydrogen (H2) production. While the assay is adaptable to various physical configurations, we describe its use in a 96-well, microtiter plate format with a lower plate containing H2-producing cyanobacteria ...
Paul S, Schrader +2 more
openaire +2 more sources
Complex phenotypic assays in high-throughput screening
Current Opinion in Chemical Biology, 2004High-throughput screening (HTS), systematically testing thousands of small molecules to find candidates for lead optimization, primarily involves exposure of purified proteins to arrayed collections of small molecules. More complex phenotypic assays, such as cell-based or whole-organism assays, traditionally have flanked HTS, preceding it to validate ...
openaire +2 more sources
Introduction: Cell-Based Assays for High-Throughput Screening
2009Cell-based assays represent approximately half of all high-throughput screens (HTS) currently performed. Here we review the history and status of HTS, and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS, drawing upon themes that will reemerge in subsequent chapters in this book.
W Frank, An, Nicola J, Tolliday
openaire +2 more sources
Design and Implementation of High-Throughput Screening Assays
2016HTS remains at the core of the drug discovery process, and so it is critical to design and implement HTS assays in a comprehensive fashion involving scientists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful consideration of many options and variables, starting with the choice of screening strategy and ...
David J, Powell +2 more
openaire +2 more sources
Fluorescence Assays for High-Throughput Screening of Protein Kinases
Combinatorial Chemistry & High Throughput Screening, 2003Protein kinases comprise one of the most important group of targets for drug discovery research today. Methods to identify novel kinase inhibitors by high-throughput screening have evolved rapidly in recent years. An important aspect is the availability of fluorescent probes that can be applied in a homogeneous, or mix-and-measure, assay format.
G J R, Zaman +3 more
openaire +2 more sources
High-Throughput Screening Assays for Dengue Antiviral Drug Development
2021Dengue is an arthropod-borne viral disease that has become endemic and a global threat in over 100 countries. The increase in prevalence would require a long-term measure to control outbreaks. Sanofi Pasteur has licensed the tetravalent dengue vaccine (Dengvaxia) in certain dengue endemic countries.
Shobana Gabriel, Jabanathan +2 more
openaire +2 more sources
Cell-based assays for high-throughput screening
Expert Opinion on Drug Discovery, 2006The fifth international World Pharmaceutical Congress was organised by the Cambridge Healthtech Institute and contained six concurrent conferences in parallel. This report focuses on the third annual conference: 'Cell-based assays for HTS'. The major topics of this meeting were technologies for setting up screening assays, cells as biological reagents ...
openaire +2 more sources
A Colloidal Stability Assay Suitable for High-Throughput Screening
Analytical Chemistry, 2016A library of 32 polystyrene copolymer latexes, with diameters ranging between 53 and 387 nm, was used to develop and demonstrate a high-throughput assay using a 96-well microplate platform to measure critical coagulation concentrations, a measure of colloidal stability.
Carla, Abarca +4 more
openaire +2 more sources
High-throughput screening assay for d-amino acid oxidase
Analytical Biochemistry, 2008A membrane-based high-throughput screening (HTS) assay for active D-amino acid oxidase (DAAO) in liquid samples as well as in intact Escherichia coli cells has been developed and optimized. The detection limit of the assay was less than 1 ng per sample.
Svetlana V, Khoronenkova +1 more
openaire +2 more sources