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A miniaturized high-throughput screening assay for fucosyltransferase VII
Analytical Biochemistry, 2008Fucosyltransferase VII (FucTVII) is a very promising drug target for treatment of inflammatory skin diseases. Its activity is required for synthesis of the sialyl-Lewis X glycoepitopes on the E- and P-selectin ligands, necessary for lymphocyte migration into the skin.
Oliver von, Ahsen +7 more
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High throughput screening – considerations for enzyme assays
1992Abstract The rapidly increasing number of potential therapeutic targets is leading the pharmaceutical industry to radically alter its drug discovery strategy (for reviews, see 1–6). For these targets, there is often little or no structural information available for the rational design of putative drug candidates. Additionally, there is a
David Hayes, Geoff Mellor
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A fluorescence-based glycosyltransferase assay for high-throughput screening
Bioorganic & Medicinal Chemistry, 2014Glycosyltransferases catalyze the transfer of a monosaccharide unit from a nucleotide or lipid sugar donor to polysaccharides, lipids, and proteins in a stereospecific manner. Considerable effort has been invested in engineering glycosyltransferases to diversify sugar-containing drugs. An important requirement for glycosyltransferase engineering is the
Jihye, Ryu +4 more
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Phosphatase High-Throughput Screening Assay Design and Selection
2013Phosphatases are a heterogeneous group of enzymes catalyzing dephosphorylation of diverse substrates ranging from small organic molecules to large phosphorylated multiprotein complexes. A wide variety of biochemical approaches for measuring phosphatase activity exists.
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A high-throughput screening assay for hydroxynitrile lyase activity
Chemical Communications, 2006A high-throughput screening assay for hydroxynitrile lyase activity accepting a wide range of HNL-substrates is presented, which is useful either for enzyme fingerprinting or screening of huge variant libraries generated in metagenome or directed evolution approaches.
Andexer, Jennifer +3 more
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Microplate assays for high-throughput screening.
Current opinion in drug discovery & development, 2009Microplates containing an 8 x 12 array of 96-wells are the most prevalent format for screening compound libraries in high-throughput applications today. These plates are suitable for use in a wide variety of homogeneous and heterogeneous assays to measure binding interactions, enzyme activity and cell proliferation.
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