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Cell-based assays for high-throughput screening
Expert Opinion on Drug Discovery, 2006The fifth international World Pharmaceutical Congress was organised by the Cambridge Healthtech Institute and contained six concurrent conferences in parallel. This report focuses on the third annual conference: 'Cell-based assays for HTS'. The major topics of this meeting were technologies for setting up screening assays, cells as biological reagents ...
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A Colloidal Stability Assay Suitable for High-Throughput Screening
Analytical Chemistry, 2016A library of 32 polystyrene copolymer latexes, with diameters ranging between 53 and 387 nm, was used to develop and demonstrate a high-throughput assay using a 96-well microplate platform to measure critical coagulation concentrations, a measure of colloidal stability.
Carla, Abarca +4 more
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High-throughput screening assay for d-amino acid oxidase
Analytical Biochemistry, 2008A membrane-based high-throughput screening (HTS) assay for active D-amino acid oxidase (DAAO) in liquid samples as well as in intact Escherichia coli cells has been developed and optimized. The detection limit of the assay was less than 1 ng per sample.
Svetlana V, Khoronenkova +1 more
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A miniaturized high-throughput screening assay for fucosyltransferase VII
Analytical Biochemistry, 2008Fucosyltransferase VII (FucTVII) is a very promising drug target for treatment of inflammatory skin diseases. Its activity is required for synthesis of the sialyl-Lewis X glycoepitopes on the E- and P-selectin ligands, necessary for lymphocyte migration into the skin.
Oliver von, Ahsen +7 more
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High throughput screening – considerations for enzyme assays
1992Abstract The rapidly increasing number of potential therapeutic targets is leading the pharmaceutical industry to radically alter its drug discovery strategy (for reviews, see 1–6). For these targets, there is often little or no structural information available for the rational design of putative drug candidates. Additionally, there is a
David Hayes, Geoff Mellor
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Phosphatase High-Throughput Screening Assay Design and Selection
2013Phosphatases are a heterogeneous group of enzymes catalyzing dephosphorylation of diverse substrates ranging from small organic molecules to large phosphorylated multiprotein complexes. A wide variety of biochemical approaches for measuring phosphatase activity exists.
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A high-throughput screening assay for hydroxynitrile lyase activity
Chemical Communications, 2006A high-throughput screening assay for hydroxynitrile lyase activity accepting a wide range of HNL-substrates is presented, which is useful either for enzyme fingerprinting or screening of huge variant libraries generated in metagenome or directed evolution approaches.
Andexer, Jennifer +3 more
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Microplate assays for high-throughput screening.
Current opinion in drug discovery & development, 2009Microplates containing an 8 x 12 array of 96-wells are the most prevalent format for screening compound libraries in high-throughput applications today. These plates are suitable for use in a wide variety of homogeneous and heterogeneous assays to measure binding interactions, enzyme activity and cell proliferation.
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