Results 101 to 110 of about 902,297 (302)

High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution

bioRxiv, 2018
Targeted PCR amplification and high-throughput sequencing (amplicon sequencing) of 16S rRNA gene fragments is widely used to profile microbial communities.
B. Callahan, J. Wong, C. Heiner, Steve Oh, C. Theriot, Ajay S. Gulati, Sarah K. McGill, Michael K. Dougherty   +7 more
semanticscholar   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

High-throughput sequencing of 16S rDNA amplicons characterizes bacterial composition in cerebrospinal fluid samples from patients with purulent meningitis

Drug Design, Development and Therapy, 2015
Aicui Liu,1,2,* Chao Wang,1,2,* Zhijuan Liang,3 Zhi-Wei Zhou,4 Lin Wang,1,2 Qiaoli Ma,1,2 Guowei Wang,1,2 Shu-Feng Zhou,4 Zhenhai Wang1,2 1Neurology Center, General Hospital of Ningxia Medical University, Yinchuan, Ningxia; 2Key Laboratory of Brain ...
Liu A, Wang C, Liang Z, Zhou ZW, Wang L, Ma Q, Wang G, Zhou SF, Wang Z   +8 more
doaj  

Massively multiplex single-molecule oligonucleosome footprinting

eLife, 2020
Our understanding of the beads-on-a-string arrangement of nucleosomes has been built largely on high-resolution sequence-agnostic imaging methods and sequence-resolved bulk biochemical techniques. To bridge the divide between these approaches, we present
Nour J Abdulhay, Colin P McNally, Laura J Hsieh, Sivakanthan Kasinathan, Aidan Keith, Laurel S Estes, Mehran Karimzadeh, Jason G Underwood, Hani Goodarzi, Geeta J Narlikar, Vijay Ramani   +10 more
doaj   +1 more source

Emerging insights into CC and CXC chemokines and their receptors in Mycobacterium tuberculosis infection

FEBS Open Bio, EarlyView.
The dual roles of CC and CXC chemokines in distinguishing active, latent, and subclinical tuberculosis were reviewed, along with an evaluation of their potential as diagnostic biomarkers and therapeutic targets to advance precision medicine in tuberculosis management. The graphical abstract was generated with AI assistance (Gemini 3.0).
Xuying Yin, Dangsheng Xiao, Jiezuan Yang
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

FACETS: allele-specific copy number and clonal heterogeneity analysis tool for high-throughput DNA sequencing

Nucleic Acids Research, 2016
Allele-specific copy number analysis (ASCN) from next generation sequencing (NGS) data can greatly extend the utility of NGS beyond the identification of mutations to precisely annotate the genome for the detection of homozygous/heterozygous deletions ...
R. Shen, V. Seshan
semanticscholar   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Metagenomic Sequencing of Maize Reveals Abundant Genomic RNA of a Comovirus, a Genus Previously Known to Infect Only Dicots [PDF]

The Plant Pathology Journal
To better understand the diversity of viral pathogens in Türkiye, a major exporter of cereals in Europe, we performed high-throughput sequencing of total RNA from maize plants collected in the Trakya region.
Havva Ilbağı, Surapathrudu Kanakala, Rick Masonbrink, Zachary Lozier, W. Allen Miller   +4 more
doaj   +1 more source

Aging Is a Key Driver for Adult Acute Myeloid Leukemia

Aging and Cancer, EarlyView.
Acute myeloid leukemia (AML) is a classical age‐related hematologic malignancy, and a key driver of AML is aging, which profoundly regulates intrinsic factors such as genomic instability, epigenetic reprogramming, and metabolic dysregulation, and alters bone marrow microenvironment.
Rong Yin, Haojian Zhang
wiley   +1 more source