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High throughput DNA sequencing: The new sequencing revolution
Plant Science, 2010Improvements in technology have rapidly changed the field of DNA sequencing. These improvements are boosted by bio-medical research. Plant science has benefited from this breakthrough, and a number of plant genomes are now available, new biological questions can be approached and new breeding strategies can be designed.
Michel, Delseny, Bin, Han, Yue Ie, Hsing
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Analysis of High-Throughput Sequencing Data
2010Next-generation sequencing has revolutionized biology by exponentially increasing sequencing output while dramatically lowering costs. High-throughput sequence data with shorter reads has opened up new applications such as whole genome resequencing, indel and SNP detection, transcriptome sequencing, etc.
Shrinivasrao P, Mane +2 more
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Applications of Ultra-high-Throughput Sequencing
2009The genomics era has enabled scientists to more readily pose truly global questions regarding mutation, evolution, gene and genome structure, function, and regulation. Just as Sanger sequencing ushered in a paradigm shift that enabled the molecular basis of biological questions to be directly addressed, to an even greater degree, ultra-high-throughput ...
Samuel, Fox +2 more
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DNA sequencing in high-throughput neuroanatomy
Journal of Chemical Neuroanatomy, 2019Mapping brain connectivity at single cell resolution is critical for understanding brain structure. For decades, such mapping has been principally approached with microscopy techniques, aiming to visualize neurons and their connections. However, these techniques are often very labor intensive and do not scale well to the complexity of mammalian brains.
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Viral Detection by High-Throughput Sequencing
2014We applied a high-throughput sequencing platform, Ion PGM, for viral detection in fecal samples from adult cows collected in Hokkaido, Japan. Random RT-PCR was performed to amplify RNA extracted from 0.25 ml of fecal specimens (N = 8), and more than 5 μg of cDNA was synthesized. Unbiased high-throughput sequencing using the 318 v2 semiconductor chip of
Daisuke, Motooka +3 more
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High-Throughput Sequencing Technologies
2021Optical and biochemical methods determine the sequence of nucleotide bases in a DNA macromolecule. Since 2010, considerable progress has been made on DNA sequencing technology. There are various high-throughput sequencing (HTS) technologies, such as Roche 454, Illumina dye sequencing, PacBio’s SMRT, Ion Torrent, Oxford Nanopore, SOLiD, and DNA nano ...
Elakkiya Elumalai, Krishna Kant Gupta
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Nonoverlapping Clone Pooling for High-Throughput Sequencing
IEEE/ACM Transactions on Computational Biology and Bioinformatics, 2012Simultaneously sequencing multiple clones using second-generation sequencers can speed up many essential clone-based sequencing methods. However, in applications such as fosmid clone sequencing and full-length cDNA sequencing, it is important to create pools of clones that do not overlap on the genome for the identification of structural variations and
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High-throughput sequencing (HTS) for the analysis of viral populations.
Infection, Genetics and Evolution, 2020The development of High-Throughput Sequencing (HTS) technologies is having a major impact on the genomic analysis of viral populations. Current HTS platforms can capture nucleic acid variation across millions of genes for both selected amplicons and full
M. Pérez‐Losada +6 more
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Selective Gene Amplification for High-Throughput Sequencing
Recent Patents on DNA & Gene Sequences, 2009With tremendous progress in next generation sequencing technologies, it has become possible to perform genomic sequencing in a highly cost-effective manner. However, the human genome remains too complex to be routinely re-sequenced. Thus, selective gene amplification is often employed to target a subset of genomic regions (such as a set of disease ...
Ting, Ni +4 more
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Promises and pitfalls of using high‐throughput sequencing for diet analysis
Molecular Ecology Resources, 2018The application of high‐throughput sequencing‐based approaches to DNA extracted from environmental samples such as gut contents and faeces has become a popular tool for studying dietary habits of animals.
A. Alberdi +6 more
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