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High-Throughput Sequencing of Complete Mitochondrial Genomes
2016Next-generation sequencing has revolutionized mitogenomics, turning a cottage industry into a high throughput process. This chapter outlines methodologies used to sequence, assemble, and annotate mitogenomes of non-model organisms using Illumina sequencing technology, utilizing either long-range PCR amplicons or gDNA as starting template.
Andrew George, Briscoe +2 more
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High-Throughput Sequencing and Metagenomics
Estuaries and Coasts, 2009The fields of biology and environmental health are undergoing an unprecedented transformation in the way communities are characterized and studied. One of the catalysts for this change has been the advent of high-throughput sequencing (aka next-generation sequencing).
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Identification and Verification of microRNAs by High-Throughput Sequencing
2013High-throughput sequencing methods have become invaluable for detection and analysis of small RNAs. The results are millions of sequences that need to be carefully analyzed by computational methods and preferentially verified by different experimental techniques.
Hällman J +4 more
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Bioinformatics for High Throughput Sequencing
2012Preface.- Introduction.- Overview of Sequencing Technology Platforms.- Applications of High Throughput Sequencing.- Computational Infrastructure and Basic Data Analysis for High-Throughput Sequencing.- Base-Calling for Bioinformaticians.- De Novo Short Read Assembly.- Short Read Mapping.- DNA-Protein Interaction Analysis (ChIP-Seq).- Genome-wide ...
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Computational Analysis of High Throughput Sequencing Data
2011The advent of High Throughput Sequencing (HTS) methods opens new opportunities for the analysis of genomes and transcriptomes. While the sequencing of a whole mammalian genome took several years at the turn of this century, today it is only a matter of weeks. The race towards the thousand-dollar genome is fueled by the - ethically challenging - idea of
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Use of Robotics in High-Throughput DNA Sequencing
2010Until relatively recently, full sequencing of genes consisting of more than several exons was not considered practicable within a routine diagnostic context. As a result, many approaches to unknown mutation detection in a specific gene involved a mutation pre-screening step to limit the amount of DNA sequencing required.
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มะเร็งปากมดลูกมีสาเหตุหลักมาจากการติดเชื้อไวรัสฮิวแมนปาปิลโลมาในกลุ่ม high risk genotype แต่สามารถพบการติดเชื้อในกลุ่ม low risk genotype ในผู้ป่วยมะเร็งปากมดลูกด้วย ซึ่งเทคนิคที่ใช้ในปัจจุบันไม่สามารถตรวจพบได้ ในขณะที่เทคโนโลยีที่มีประสิทธิภาพสูงอย่าง high-throughput sequencing สามารถตรวจพบสายพันธุ์ของไวรัสได้คลอบคลุมมากกว่าแต่มีข้อเสียในเรื่องค่าใช้จ ...
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