Results 21 to 30 of about 22,206,764 (294)

Optimization of lutein-zeaxanthine separation on high performance liquid chromatography

open access: yesNotulae Botanicae Horti Agrobotanici Cluj-Napoca, 1993
Separation of lutein and zeaxanthine which are carotenoidic isomeric pigments on High Performance Liquid Chromatography (HPLC) was investigated. Three different columns were used to separate standard mixtures: a normal phase silica column, a reversed ...
Crenguta TABACARU   +2 more
doaj   +1 more source

Determination of secoisolariciresinol diglucoside by high performance liquid chromatography

open access: yes浙江大学学报. 农业与生命科学版, 2008
High performance liquid chromatography-photodiode array detection-elctrospray ionizationmass spectrometry (HPLC-PAD-ESI-MS) method was used to determine secoisolariciresinol diglucoside (SDG) qualitatively and quantitatively. Precision, stability, limits
ZHANG Wen-bin   +4 more
doaj   +1 more source

Development and validation of an HPLC-UV method for purity determination of DNA

open access: yesMedicine Science, 2023
Determination of the concentration and the purity of DNAs is crucial for measuring the DNA copy number since it will influence further DNA analysis such as digital PCR (dPCR) and next-generation sequencing (NGS).
Merve Oztug, Burhanettin Yalcinkaya
doaj   +1 more source

Measurement of stable isotopic enrichment and concentration of long-chain fatty acyl-carnitines in tissue by HPLC-MS

open access: yesJournal of Lipid Research, 2006
We have developed a new method for the simultaneous measurements of stable isotopic tracer enrichments and concentrations of individual long-chain fatty acyl-carnitines in muscle tissue using ion-pairing high-performance liquid chromatography ...
Dayong Sun   +4 more
doaj   +1 more source

New HPLC method for determination of fluvastatin anti-hyperlipidemic drug in bulk and pharmaceutical dosage using simvastatin as internal standard

open access: yesDrug Analytical Research, 2023
A new, and reliable RP–HPLC method has been investigated and validated for the assay of fluvastatin sodium (FVS) in pharmaceutical capsules. The chromatographic method was carried out using an ODS Hypersil C18 column (250×4.6 mm i.d., 5 µm particle size)
Safwan Ashour, Mouhammed Khateeb
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

open access: yesFEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa   +5 more
wiley   +1 more source

Analysis of total urinary catecholamines by liquid chromatography: methodology, routine experience and clinical interpretations of results [PDF]

open access: yes, 1983
A simple routine method is described for simultaneous assay of total urinary adrenaline, noradrenaline and dopamine. The catecholamines are pre-purified on a small ion-exchange column, separated by reversed phase ion-pair liquid chromatography, and are ...
Schleicher, E. D.   +2 more
core   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

open access: yesFEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto   +3 more
wiley   +1 more source

Determination of stigmasterol hydroperoxides using high-performance liquid chromatography-mass spectrometry with atmospheric pressure chemical ionization

open access: yesCzech Journal of Food Sciences, 2004
A new specific method using high-performance liquid chromatography-mass spectrometry (HPLC-MS) for the detection of stigmasterol hydroperoxides was developed.
S. Kemmo   +3 more
doaj   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

open access: yesFEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens   +8 more
wiley   +1 more source

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