Results 41 to 50 of about 445,213 (310)

Temperature-Controlled Microchip Liquid Chromatography System [PDF]

, 2006
High-performance liquid chromatography (HPLC) is one of the most important analytical tools heavily used in the fields of chemistry, biotechnology, pharmaceutics, and the food industry.
Shih, Victor-Chi-Yuan
core   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 3. Comparison of HPLC and UHPLC determination of α- and β-acids.

Kvasný průmysl, 2012
The traditional method of High Performance Liquid Chromatography (HPLC) was compared with the currently increasingly used Ultra- High Performance Liquid Chromatography (UHPLC) on a representative group of 11 hop samples.
Marie JURKOVÁ, Pavel ČEJKA, Jana OLŠOVSKÁ   +2 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Quantification of norisoboldine in Linderae Radix by ultra-performance liquid chromatography and high performance liquid chromatography with UV detection

, 2012
Norisoboldine is an alkaloid and has been identified as the major active component in Linderae Radix. A novel method for quantitative analysis of norisoboldine in Linderae Radix using ultra performance liquid chromatography (UPLC) with UV detection was ...
Chou, G.-X., Wang, Z., Yang, L., Bligh, S.W.A., Chen, J.Z.   +4 more
core   +1 more source

Analysis of sphingoid bases by reversed-phase high performance liquid chromatography.

Journal of Lipid Research, 1983
A reversed-phase high performance liquid chromatography (HPLC) technique was developed for the analysis of sphingoid bases as their biphenylcarbonyl derivatives.
F B Jungalwala, J E Evans, E Bremer, R H McCluer   +3 more
doaj   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Analysis of aminophospholipid molecular species by high performance liquid chromatography.

Journal of Lipid Research, 1989
A new method is described for the separation of individual molecular species of the aminophospholipids, phosphatidylethanolamine and phosphatidylserine. Trinitrobenzene-sulfonic acid was used to derivatize both aminophospholipids and the derivatives were
F Hullin, H Y Kim, N Salem, Jr
doaj   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Development and validation of high-performance liquid chromatographic method for determination of ofloxacin and lomefloxacin in human plasma [PDF]

Journal of the Serbian Chemical Society, 2005
Ahigh-performance liquid chromatographicmethod for the determination of ofloxacin and lomefloxacin in human plasma has been developed and validated. The effect of organic modifiers on the retention of the investigated drugs was investigated.
Zendelovska Dragica, Stafilov Trajče
doaj   +1 more source