Results 61 to 70 of about 445,213 (310)

PAK1 activation drives divergent resistance mechanisms to aromatase inhibition and tamoxifen in a luminal: A breast cancer model

Molecular Oncology, EarlyView.
Breast cancer remains a major cause of cancer death in women, frequently developing endocrine therapy resistance. This study demonstrates that upregulated p21‐activated kinase 1 (PAK1) activity drives resistance to tamoxifen and long‐term estrogen deprivation in ER+ breast cancer models.
Luisa Schwarzmüller, Janina Müller, Efstathios‐Iason Vlachavas, Lukas Beumers, Lena Fleischhacker, Sara Burmester, Angelika Wörner, Sabine Karolus, Dominic Helm, Cindy Körner, Stefan Wiemann   +10 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

MYCOBACTERIUM COMPLEX IDENTIFICATION BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Journal of Research in Medical Sciences, 2001
Introduction: There are different ways for identification of Mycobacteria. One of the most sensitive method is HPLC of phenacyl esters of mycolic acids of Mycobacteria for rapid identification of them after their primary cultures.
S.A HAWAII, T NASERPUR-FARIVAR, G.A NADERI, H TAMIZI FAR   +3 more
doaj  

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

ANALYSIS OF URINARY PORPHYRINS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION MASS SPECTROMETRY

, 2011
A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS) method was developed for the simultaneous separation and detection of six porphyrins, namely uroporphyrin (UP), heptacarboxylic acid porphyrin ...
Chunyang Liao, Lingxin Chen, Xu, Shoufang, Cai, Zongwei, Chen, Lingxin, Li, Jinhua, Song, Xingliang, Xingliang Song, Zongwei Cai, Liao, Chunyang, Jinhua Li, Shoufang Xu   +11 more
core   +1 more source

Determination of melamine in biscuits by high performance liquid chromatography

浙江大学学报. 农业与生命科学版, 2010
A high performance liquid chromatography method was developed for the determination of melamine in biscuits. The biscuit samples were extracted by 1% trichloroacetic acid solution and 2.2% lead acetate, and melamine was separated on a Hypersil ODS C18 ...
WEI Jin-mei, ZHANG Yong-mei, WANG Ji-qing, CAO Lu-xing, LUO Yu-zhu   +4 more
doaj   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

RECENT ADVANCES IN GAS, LIQUID AND SUPERCRITICAL FLUID CHROMATOGRAPHY IN THE CONTEXT OF SUSTAINABLE/ GREEN CHEMISTRY

International Journal of Pharmacy & Integrated Health Sciences
Background: Chromatographic techniques such as gas chromatography (GC), liquid chromatography (LC) and supercritical fluid chromatography (SFC) have long been employed in analytical chemistry. However, using or producing certain organic solvents and non-
Nimra Sohail, Bushra Naureen, Mehmood Asghar, Aziza Sarwar, Aisha Altaf   +4 more
doaj   +1 more source

Quantification of tryptophan in plasma by high performance liquid chromatography

Química Nova, 2012
A simple, rapid and selective method using high-performance liquid chromatography with ultraviolet detection (267 nm) was applied for the determination of tryptophan in plasma.
Renata Romanholi Pinhati, Hudson Caetano Polonini, Marcos Antônio Fernandes Brandão, Nádia Rezende Barbosa Raposo, Felipe Corchs, Wagner Farid Gattaz   +5 more
doaj   +1 more source

Advancements in the characterisation of oligonucleotides by high performance liquid chromatography‐mass spectrometry in 2021: A short review

Analytical Science Advances, 2022
The first oligonucleotide therapeutic was approved by the Food and Drug Administration in 1998, and since then, 12 nucleic acids have been commercialised as medicines.
Fabien Hannauer, Rachelle Black, Andrew D. Ray, Eugen Stulz, G. John Langley, Stephen W. Holman   +5 more
doaj   +1 more source