Results 81 to 90 of about 445,213 (310)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

Ion exchange chromatography – basic principles and application [PDF]

, 2011
Ion-Exchange Chromatography (IEC) allows for the separation of ionizable molecules on the basis of differences in charge properties. Its large sample-handling capacity, broad applicability (particularly to proteins and enzymes), moderate cost, powerful ...
Dowling, Oonagh, Cummins, Phil, O'Connor, Brendan, O\u27Connor, Brendan   +3 more
core  

High-performance liquid chromatographic separation of metal carbonyl complexes substituted with bridging and chelating 1,1'-bis(diphenylphosphino)ferrocene

, 1991
The high-performance liquid chromatography of metal carbonyl complexes including those containing the 1,1'-bis(diphenylphosphino)ferrocene ligand is reported. Separations were performed on a column packed with silica bonded with polar amino-cyano groups.
Phang, L. T., Hor, T. S A, Lee, H. K., Koo, L. K.   +3 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Advanced modelling and visualisation of liquid-liquid separations of complex sample components, with variable phase distribution and mode of operation

, 2013
This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.This research is about liquid-liquid chromatography modelling.
De Folter, Jozefus Johannes Martinus
core  

Direct synthesis of hierarchical monolithic silica for high performance liquid chromatography

, 2008
The silica-based monolith exhibiting a hierarchical bimodal porous structure has been directly synthesized via lytropic mesophase. The hydrolysis and condensation of tetramethoxysilane (TMOS) in the presence of poly(ethylene oxide)-block-poly(propylene ...
Zhu, Guiru, Liu, Jian, Yang, Jie, 杨启华, Zhong, Hua, Wang, Peiyuan, Yang, Qihua   +6 more
core   +1 more source

An on-line solid phase extraction procedure for the routine quantification of urinary methylmalonic acid by liquid chromatography-tandem mass spectrometry [PDF]

, 2010
Background: The goal of this study was to develop and to validate an improved isotope-dilution-liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of methylmalonic acid (MMA) in urine.
Michael Vogeser, Lorenzl, Stephan, Stephan Lorenzl, Fabian Kirchhoff, Vogeser, Michael, Kirchhoff, Fabian   +5 more
core   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source