Results 61 to 70 of about 693,447 (358)

Denaturing high‐performance liquid chromatography: A review

open access: yesHuman Mutation, 2001
Denaturing high‐performance liquid chromatography (DHPLC) compares two or more chromosomes as a mixture of denatured and reannealed PCR amplicons, revealing the presence of a mutation by the differential retention of homo‐ and heteroduplex DNA on ...
W. Xiao, Peter J. Oefner
semanticscholar   +1 more source

Gut alterations in a chronic kidney disease rat model with diet‐induced vascular calcification

open access: yesFEBS Open Bio, EarlyView.
Chronic kidney disease (CKD) patients often suffer from intestinal and/or mineral and bone disorders. Using a rat model, we showed that uremic vascular calcification is associated with gut barrier alterations (decreased gut mucus production and Nlrp6 gene expression, increased gut inflammation), and plasma retention of gut‐origin uremic toxins (indoxyl
Piotr Bartochowski   +13 more
wiley   +1 more source

High Performance Liquid Chromatography(pdf)

open access: yes, 2018
Kromatografi Cair Kinerja Tinggi (KCKT), High Performance Liquid Chromatography (HPLC) merupakan salah satu metode kimia dan fisikokimia. HPLC adalah kromatografi cair kolom modern, dimana teori dasarnya bukanlah baru tetapi hasil pengembangan dari kromatografi cair kolom ...
openaire   +1 more source

Iron‐dependent lysosomal LDL oxidation induces the expression of scavenger receptor A in human THP‐1 monocytes

open access: yesFEBS Open Bio, EarlyView.
In human monocytic cells THP‐1, a limited uptake of native—not oxidized—LDL/VLDL induced expression of scavenger receptor A and cellular adhesion. Induction was inhibited by lysosomotropic (WR‐1065) and lipophilic (BHT) antioxidants and by siRNAs against ferritinophagy.
Martina Čierna   +4 more
wiley   +1 more source

Quantification of gangliosides by microbore high performance liquid chromatography.

open access: yesJournal of Lipid Research, 1996
A highly sensitive analytical method was developed that allows the separation of ganglioside mixtures and quantification of individual non-derivatized gangliosides in the concentration range between 2 pmol and 1 nmol.
R Wagener, B Kobbe, W Stoffel
doaj  

A Review on High Performance Liquid Chromatography (HPLC)

open access: yesAsian Journal of Pharmaceutical Analysis, 2016
The estimate of pharmaceutical and biological materials typically uses the key qualitative and quantitative technique known as high performance liquid chromatography (HPLC).
Mukthi Thammana
semanticscholar   +1 more source

GDP‐fucose transporter SLC35C1: a potential regulatory role in cytosolic GDP‐fucose and fucosylated glycan synthesis

open access: yesFEBS Open Bio, EarlyView.
The inactivation of SLC35C1 (GDP‐fucose transporter) and enzymes involved in GDP‐fucose biosynthesis was studied. Fucose supplementation increases the level of GDP‐fucose to abnormal, millimolar values in the absence of the TSTA3 protein and SLC35C1 in contrast to the GMDS/SLC35C1 double mutant.
Edyta Skurska, Mariusz Olczak
wiley   +1 more source

Quantification of tryptophan in plasma by high performance liquid chromatography

open access: yesQuímica Nova, 2012
A simple, rapid and selective method using high-performance liquid chromatography with ultraviolet detection (267 nm) was applied for the determination of tryptophan in plasma.
Renata Romanholi Pinhati   +5 more
doaj   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

open access: yesFEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Measurement of stable isotopic enrichment and concentration of long-chain fatty acyl-carnitines in tissue by HPLC-MS

open access: yesJournal of Lipid Research, 2006
We have developed a new method for the simultaneous measurements of stable isotopic tracer enrichments and concentrations of individual long-chain fatty acyl-carnitines in muscle tissue using ion-pairing high-performance liquid chromatography ...
Dayong Sun   +4 more
doaj  

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