Results 41 to 50 of about 13,156 (264)

Method transfer from high-pressure liquid chromatography to ultra-high-pressure liquid chromatography. II. Temperature and pressure effects

Journal of Chromatography A, 2015
The importance of the generated temperature and pressure gradients in ultra-high-pressure liquid chromatography (UHPLC) are investigated and compared to high-pressure liquid chromatography (HPLC). The drug Omeprazole, together with three other model compounds (with different chemical characteristics, namely uncharged, positively and negatively charged)
Åsberg, D., Samuelsson, J., Leśko, M., CAVAZZINI, Alberto, Kaczmarski, K., Fornstedt, T.   +5 more
openaire   +3 more sources

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

High pressure thermal conductivity of three ethyl esters in the liquid phase

Case Studies in Thermal Engineering, 2021
Recently, biodiesel is considered as a biodegradable, renewable and high-potential fuel which could be used directly in tradition internal combustion engines.
Jing Fan, Han Mu, Chang Gao, Fenhong Song   +3 more
doaj   +1 more source

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

Separation of the natural retinoids by high-pressure liquid chromatography

Journal of Lipid Research, 1978
A reverse phase high-pressure liquid chromatography system for rapid separation of various retinoids (vitamin A and its analogs) with little or no degradation is described. This method permits detection of as little as 22 pmol of retinoic acid.
C A Frolik, T E Tavela, M B Sporn
doaj   +1 more source

Hijacking emergency granulopoiesis: Neutrophil ontogeny and reprogramming in cancer

Molecular Oncology, EarlyView.
Neutrophils are highly plastic innate immune cells; their functions in cancer extend beyond the tumour microenvironment. This Review summarises current understanding of neutrophil maturation and heterogeneity and highlights tumour‐induced granulopoiesis as a systemic programme that expands immature, immunosuppressive neutrophils via tumour‐derived ...
Gabriela Marinescu, Yi Feng
wiley   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

Cavitation upon low-speed solid–liquid impact

Nature Communications, 2021
A solid hitting a liquid surface normally creates a region of high pressure at the solid-liquid contact area. Now, it is shown that for a flat-bottomed cylinder hitting a liquid at low-enough impact speed, the local pressure is sufficiently low to cause ...
Nathan B. Speirs, Kenneth R. Langley, Zhao Pan, Tadd T. Truscott, Sigurdur T. Thoroddsen   +4 more
doaj   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Occurrence and Exposure Assessment of Aflatoxin B1 and Ochratoxin A in Pearl Millet (Pennisetum glaucum L.) from Tunisia

Journal of Food Quality and Hazards Control, 2020
Background: Ochratoxin A (OTA) and Aflatoxin B1 (AFB1) are toxic secondary  metabolites produced by certain mold species. In this primarily survey, we examined the OTA and AFB1 contamination of pearl millet grains distributed in Tunisia.
S. Lasram, Z. Hamdi, A. Ghorbel
doaj