Results 31 to 40 of about 311,623 (216)

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Compact Spatial Heterodyne Spectrographs for Future Space-Based Observations: Instrument Modeling and Applications

Sensors
High-resolution spectroscopy employing spatial heterodyne spectrographs (SHS) holds significant promise for forthcoming space missions, building upon its established track record in science applications.
Ayan Sahoo, Joice Mathew, Andrew Battisti, Brad Tucker   +3 more
doaj   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

SDSS-V Milky Way Mapper (MWM): ASPCAP Stellar Parameters and Abundances in SDSS-V Data Release 19

The Astronomical Journal
The goal of this paper is to describe the science verification of Milky Way Mapper (MWM) APOGEE Stellar Parameter and Chemical Abundances Pipeline (ASPCAP) data products published in Data Release 19 (DR19) of the fifth phase of the Sloan Digital Sky ...
Szabolcs Mészáros, Paula Jofré, Jennifer A. Johnson, Jonathan C. Bird, Jo Bovy, Andrew R. Casey, Julio Chanamé, Katia Cunha, Nathan De Lee, Peter Frinchaboy, Guillaume Guiglion, Viola Hegedűs, Alex P. Ji, Juna A. Kollmeier, Melissa K. Ness, Jonah Otto, Marc H. Pinsonneault, Alexandre Roman-Lopes, Andrew Saydjari, Amaya Sinha, Ying-Yi Song, Guy S. Stringfellow, Keivan G. Stassun, Jamie Tayar, Andrew Tkachenko, Marica Valentini, Zachary Way, Jörg Weingrill   +27 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

High-Resolution Localized NMR Spectroscopy Based on Hadamard-Encoding

Chinese Journal of Magnetic Resonance, 2020
Nuclear magnetic resonance (NMR) is a non-invasive technology which has been widely used in chemical, biological and medical researches. This paper presents a new NMR pulse sequence based on Hadamard spatial encoding and intermolecular single-quantum ...
KE Han-ping, CAI Hong-hao
doaj   +1 more source

Chiral separation of chloroalkanes with the chromatographic column onboard Martian rovers

FEBS Letters, EarlyView.
Computer image of the Rosalind Franklin Rover of ESA's ExoMars mission. ExoMars is scheduled to land on planet Mars in Oxia Planum in 2029. This area represents an interesting spot to look for biosignatures. Investigations of ExoMars include measurement on molecular chirality. We show that chiral chloroalkanes, that have been identified on Mars, can be
Asma Merzougui, Agnes Banreti, Armelle L. Ernst, Guillaume Leseigneur, Uwe J. Meierhenrich   +4 more
wiley   +1 more source

Smaller is better: nanobodies meet NMR

FEBS Letters, EarlyView.
Nanobodies are single‐domain antigen‐binding fragments derived from camelid heavy chain antibodies. Their small size, high stability, and exceptional specificity make nanobodies uniquely useful probes for NMR studies of protein dynamics, transient conformational states, and protein–protein interactions.
Oleg Y. Dmitriev
wiley   +1 more source

LiYF4:Ho crystal in a magnetic field: high resolution optical spectroscopy

EPJ Web of Conferences, 2017
Low-temperature high-resolution optical spectra of LiYF4:Ho in an external magnetic field H are presented. A field-dependent transformation of the hyperfine structure of holmium spectral lines is studied for the cases H||c and H┴c.
Boldyrev K.N., Popova M.N.
doaj   +1 more source

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

FEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source