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High-Throughput Sequencing Technologies
2021Optical and biochemical methods determine the sequence of nucleotide bases in a DNA macromolecule. Since 2010, considerable progress has been made on DNA sequencing technology. There are various high-throughput sequencing (HTS) technologies, such as Roche 454, Illumina dye sequencing, PacBio’s SMRT, Ion Torrent, Oxford Nanopore, SOLiD, and DNA nano ...
Elakkiya Elumalai, Krishna Kant Gupta
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2011
Advances in DNA sequencing methods have a major impact on several biological fields, with microbiology being the first field to experience the benefits of knowing the exact sequence of a genome. This advantage held by the microbiology field was a result of the more compact nature of many microbial genomes, the long-standing history of microbes as model
Parla, J. +2 more
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Advances in DNA sequencing methods have a major impact on several biological fields, with microbiology being the first field to experience the benefits of knowing the exact sequence of a genome. This advantage held by the microbiology field was a result of the more compact nature of many microbial genomes, the long-standing history of microbes as model
Parla, J. +2 more
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High-throughput sequencing and food microbiology
2020Massive parallel sequencing (High-Throughput Sequencing, HTS) permits reading of sequenced millions to billions short DNAs in parallel (reads) and is revolutionizing microbiology and food safety research from the laboratory methods to computational analysis, with the inevitable use of Bioinformatics.
Narciso M, Quijada +2 more
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Qualifying high-throughput immune repertoire sequencing
Cellular Immunology, 2014Diversity of B and T cell receptors, achieved by gene recombination and somatic hypermutation, allows the immune system for recognition and targeted reaction against various threats. Next-generation sequencing for assessment of a cell's gene composition and variation makes deep analysis of one individual's immune spectrum feasible. An easy to apply but
Norbert Niklas +7 more
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High throughput DNA sequencing: The new sequencing revolution
Plant Science, 2010Improvements in technology have rapidly changed the field of DNA sequencing. These improvements are boosted by bio-medical research. Plant science has benefited from this breakthrough, and a number of plant genomes are now available, new biological questions can be approached and new breeding strategies can be designed.
Michel, Delseny, Bin, Han, Yue Ie, Hsing
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Viral Detection by High-Throughput Sequencing
2014We applied a high-throughput sequencing platform, Ion PGM, for viral detection in fecal samples from adult cows collected in Hokkaido, Japan. Random RT-PCR was performed to amplify RNA extracted from 0.25 ml of fecal specimens (N = 8), and more than 5 μg of cDNA was synthesized. Unbiased high-throughput sequencing using the 318 v2 semiconductor chip of
Daisuke, Motooka +3 more
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Applications of Ultra-high-Throughput Sequencing
2009The genomics era has enabled scientists to more readily pose truly global questions regarding mutation, evolution, gene and genome structure, function, and regulation. Just as Sanger sequencing ushered in a paradigm shift that enabled the molecular basis of biological questions to be directly addressed, to an even greater degree, ultra-high-throughput ...
Samuel, Fox +2 more
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QTL Mapping Using High-Throughput Sequencing
2015Quantitative trait locus (QTL) mapping in plants dates to the 1980s (Stuber et al. Crop Sci 27: 639-648, 1987; Paterson et al. Nature 335: 721-726, 1988), but earlier studies were often hindered by the expense and time required to identify large numbers of polymorphic genetic markers that differentiated the parental genotypes and then to genotype them ...
Tiffany M, Jamann +2 more
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DNA sequencing in high-throughput neuroanatomy
Journal of Chemical Neuroanatomy, 2019Mapping brain connectivity at single cell resolution is critical for understanding brain structure. For decades, such mapping has been principally approached with microscopy techniques, aiming to visualize neurons and their connections. However, these techniques are often very labor intensive and do not scale well to the complexity of mammalian brains.
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Analysis of High-Throughput Sequencing Data
2010Next-generation sequencing has revolutionized biology by exponentially increasing sequencing output while dramatically lowering costs. High-throughput sequence data with shorter reads has opened up new applications such as whole genome resequencing, indel and SNP detection, transcriptome sequencing, etc.
Shrinivasrao P, Mane +2 more
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