Results 81 to 90 of about 571,441 (299)

High‐throughput generic single‐entity sequencing using droplet microfluidics

iMeta
Single‐cell sequencing has revolutionized our understanding of cellular heterogeneity by providing a micro‐level perspective in the past decade. While heterogeneity is fundamental to diverse biological communities, existing platforms are primarily ...
Guoping Wang, Liuyang Zhao, Yu Shi, Fuyang Qu, Yanqiang Ding, Weixin Liu, Changan Liu, Gang Luo, Meiyi Li, Xiaowu Bai, Luoquan Li, Luyao Wang, Chi Chun Wong, Yi‐Ping Ho, Jun Yu   +14 more
doaj   +1 more source

SNP discovery by high-throughput sequencing in soybean

BMC Genomics, 2010
Background With the advance of new massively parallel genotyping technologies, quantitative trait loci (QTL) fine mapping and map-based cloning become more achievable in identifying genes for important and complex traits.
Wu Xiaolei, Ren Chengwei, Joshi Trupti, Vuong Tri, Xu Dong, Nguyen Henry T   +5 more
doaj   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

High-throughput sequencing of black pepper root transcriptome

BMC Plant Biology, 2012
Background Black pepper (Piper nigrum L.) is one of the most popular spices in the world. It is used in cooking and the preservation of food and even has medicinal properties.
Gordo Sheila MC, Pinheiro Daniel G, Moreira Edith CO, Rodrigues Simone M, Poltronieri Marli C, de Lemos Oriel F, da Silva Israel, Ramos Rommel TJ, Silva Artur, Schneider Horacio, Silva Wilson A, Sampaio Iracilda, Darnet Sylvain   +12 more
doaj   +1 more source

The Human Virome in Children and its Relationship to Febrile Illness [PDF]

, 2010
This study investigates the relationship of viruses to febrile illness in children. Subjects are normal children 2-36 months of age with fever along with normal children of the same age without fever, plus immunocompromised children with fever along ...
David Jaffe, Erica Sodergren, George Weinstock, Gregory Storch, Gregory Storch, Jared Muenzer, Kristine Wylie, Max Arens, Richard Buller   +8 more
core   +2 more sources

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

High-throughput sequencing of 16S rDNA amplicons characterizes bacterial composition in cerebrospinal fluid samples from patients with purulent meningitis

Drug Design, Development and Therapy, 2015
Aicui Liu,1,2,* Chao Wang,1,2,* Zhijuan Liang,3 Zhi-Wei Zhou,4 Lin Wang,1,2 Qiaoli Ma,1,2 Guowei Wang,1,2 Shu-Feng Zhou,4 Zhenhai Wang1,2 1Neurology Center, General Hospital of Ningxia Medical University, Yinchuan, Ningxia; 2Key Laboratory of Brain ...
Liu A, Wang C, Liang Z, Zhou ZW, Wang L, Ma Q, Wang G, Zhou SF, Wang Z   +8 more
doaj  

Throughput Rate Optimization in High Multiplicity Sequencing Problems [PDF]

Mixed model assembly systems assemble products (parts) of differenttypes in certain prespecified quantities. A minimal part set is a smallestpossible set of product type quantities, to be called the multiplicities,in which the numbers of assembled ...
Grigoriev,Alexander, Klundert,Joris ,van de   +1 more
core   +1 more source

Dihydroorotate dehydrogenase (DHODH) regulates trophoblast syncytialization through organelle stress–induced cellular senescence

FEBS Open Bio, EarlyView.
The inhibition of mitochondrial dihydroorotate dehydrogenase (DHODH) impairs syncytialization and induces cellular senescence via mitochondrial and endoplasmic reticulum stress in human trophoblast stem cells, elevating sFlt1/PlGF levels, a hallmark of placental dysfunction in hypertensive disorders of pregnancy.
Kanoko Yoshida, Kazuya Kusama, Ayaka Horiuchi, Yu Kawaguchi, Atsuya Tsuru, Mikihiro Yoshie, Kazuhiro Tamura   +6 more
wiley   +1 more source