Results 11 to 20 of about 50 (49)
Polycistronic coexpression and nondenaturing purification of histone octamers [PDF]
Analytical Biochemistry, 2012 Histone octamers are the basic building blocks of chromatin and are platforms for diverse genetic mechanisms. We report a simple method for preparing recombinant histone octamers by overexpressing all four histones from a single polycistronic vector followed by standard chromatography under native conditions.
Shim, Yoonjung +4 more
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Crosslinked histone octamer as a model of the nucleosome core. [PDF]
Proceedings of the National Academy of Sciences, 1977 When histones in chromatin core particles were crosslinked with dimethylsuberimidate, the resulting particles had properties closely similar to those of native core particles. A crosslinked octameric histone complex was isolated from these particles under nondenaturing conditions.
Robert T. Simpson +2 more
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High-resolution structure of the native histone octamer [PDF]
Acta Crystallographica Section F Structural Biology and Crystallization Communications, 2005 Crystals of native histone octamers (H2A-H2B)-(H4-H3)-(H3'-H4')-(H2B'-H2A') from chick erythrocytes in 2 M KCl, 1.35 M potassium phosphate pH 6.9 diffract X-rays to 1.90 A resolution, yielding a structure with an R(work) value of 18.7% and an Rfree of 22.2%.
Colin D. Reynolds +5 more
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Chronopotentiometric Analysis of Single Histones and Histone Octamer at Charged Surfaces
ChemElectroChem, 2021 AbstractHistones play an essential role in the nucleosome structure and organization. The key role of highly positively charged histones is to stabilize negatively charged DNA inside the chromatin. Histones are basic proteins containing a large amount of electroactive residues, namely, arginine and lysine.
Veronika Ostatná +4 more
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A Histone Octamer Blocks Branch Migration of a Holliday Junction [PDF]
Molecular and Cellular Biology, 1997 The Holliday junction is a key intermediate in genetic recombination. Here, we examine the effect of a nucleosome core on movement of the Holliday junction in vitro by spontaneous branch migration. Histone octamers consisting of H2A, H2B, H3, and H4 are reconstituted onto DNA duplexes containing an artificial nucleosome-positioning sequence consisting ...
Mikhail Grigoriev, Peggy Hsieh
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The Histone Octamer Is Invisible When NF-κB Binds to the Nucleosome [PDF]
Journal of Biological Chemistry, 2004 The transcription factor NF-kappaB is involved in the transcriptional control of more than 150 genes, but the way it acts at the level of nucleosomal templates is not known. Here we report on a study examining the interaction of NF-kappaB p50 with its DNA recognition sequence in a positioned nucleosome.
Angelov, D. +8 more
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The effect of Ca2+ on the stability of chicken erythrocyte histone octamers [PDF]
FEBS Letters, 1982 Histones can be extracted from chicken erythrocyte chromatin with 2 M CaCl2 10 mM Tris (pH 7.4). The core histones so extracted exist as H3–H4 tetramers and H2A–H2B dimers since calcium at >0.5 M result in dissociation of the histone octamer. Reconstitution of octamers occurs on removal of the Ca2+ by dialysis.
George G. Lindsey +4 more
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Histone Octamer Instability under Single Molecule Experiment Conditions [PDF]
Journal of Biological Chemistry, 2005 We have studied the sample concentration-dependent and external stress-dependent stability of native and reconstituted nucleosomal arrays. Whereas upon stretching a single chromatin fiber in a solution of very low chromatin concentration the statistical distribution of DNA length released upon nucleosome unfolding shows only one population centered ...
Philippe Bouvet +5 more
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Octamer reconstitution from acid‐extracted chicken erythrocyte histones [PDF]
FEBS Letters, 1983 Histone octamers have been reconstituted from acid‐extracted chicken erythrocyte histones. By the criteria of molecular size on exclusion chromatography as well as sedimentation velocity and conformational properties established by circular dichroism, fluorescence spectroscopy and imido‐ester cross‐linking, the reconstituted octamers have a structure ...
Claus von Holt +4 more
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Regulation of Transcription through Acetylation of H3K122 on the Lateral Surface of the Histone Octamer [PDF]
Cell, 2013 Histone modifications are key regulators of chromatin function. However, little is known to what extent histone modifications can directly impact on chromatin. Here, we address how a modification within the globular domain of histones regulates chromatin function.
Philipp Tropberger +18 more
openaire +4 more sources