Results 1 to 10 of about 10,982 (239)
BMC Genomics, 2023 Background Nucleosome remodeling factors regulate the occupancy and positioning of nucleosomes genome-wide through ATP-driven DNA translocation. While many nucleosomes are consistently well-positioned, some nucleosomes and alternative nucleosome ...
David C. Klein +3 more
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The insulator binding protein CTCF positions 20 nucleosomes around its binding sites across the human genome. [PDF]
PLoS Genetics, 2008 Chromatin structure plays an important role in modulating the accessibility of genomic DNA to regulatory proteins in eukaryotic cells. We performed an integrative analysis on dozens of recent datasets generated by deep-sequencing and high-density tiling ...
Yutao Fu +3 more
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Single molecule analysis of CENP-A chromatin by high-speed atomic force microscopy
eLife, 2023 Chromatin accessibility is modulated in a variety of ways to create open and closed chromatin states, both of which are critical for eukaryotic gene regulation.
Daniël P Melters +4 more
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LPS-Challenged Macrophages Release Microvesicles Coated With Histones
Frontiers in Immunology, 2018 Histones are the protein component of nucleosomes, which are the basic packing unit of chromatin. However, histones are also found in the blood, both as components of nucleosomes leaked out from dead cells, or expelled from neutrophils in the active ...
Rohini Ravindran Nair +5 more
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A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins
Molecules, 2021 We report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers.
Roberto Fabian +5 more
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Nucleosomes impede Cas9 access to DNA in vivo and in vitro
eLife, 2016 The prokaryotic CRISPR (clustered regularly interspaced palindromic repeats)-associated protein, Cas9, has been widely adopted as a tool for editing, imaging, and regulating eukaryotic genomes.
Max A Horlbeck +8 more
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Specialization of the chromatin remodeler RSC to mobilize partially-unwrapped nucleosomes
eLife, 2020 SWI/SNF-family chromatin remodeling complexes, such as S. cerevisiae RSC, slide and eject nucleosomes to regulate transcription. Within nucleosomes, stiff DNA sequences confer spontaneous partial unwrapping, prompting whether and how SWI/SNF-family ...
Alisha Schlichter +3 more
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mSystems, 2021 Leishmania donovani is a parasitic protist that causes the lethal Kala-azar fever in India and East Africa. Gene expression in Leishmania is regulated by gene copy number variation and inducible translation while RNA synthesis initiates at a small number
Janne Grünebast +3 more
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Making better CRISPR libraries
eLife, 2016 A new algorithm improves the performance of CRISPR-based genetic screens in mammals.
Shiyou Zhu, Wensheng Wei
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Mechanical properties of nucleoprotein complexes determined by nanoindentation spectroscopy
Nucleus, 2020 The interplay between transcription factors, chromatin remodelers, 3-D organization, and mechanical properties of the chromatin fiber controls genome function in eukaryotes.
Tatini Rakshit +3 more
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