Results 31 to 40 of about 28,331 (162)
Specialization of the chromatin remodeler RSC to mobilize partially-unwrapped nucleosomes
eLife, 2020 SWI/SNF-family chromatin remodeling complexes, such as S. cerevisiae RSC, slide and eject nucleosomes to regulate transcription. Within nucleosomes, stiff DNA sequences confer spontaneous partial unwrapping, prompting whether and how SWI/SNF-family ...
Alisha Schlichter +3 more
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Biomolecules, 2022 Background: Neutrophil extracellular traps’ (NETs’) formation is a mechanism of defense that neutrophils deploy as an alternative to phagocytosis, to constrain the spread of microorganisms.
Laure Morimont +14 more
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Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo
Proceedings of the National Academy of Sciences of the United States of America, 2018 Significance The efficiency of genome editing with CRISPR-Cas9 can vary widely at different targets and in different cells. Some of this variability may be due to the inherent quality of different guide RNAs, but it may also depend on the cellular ...
R. Yarrington +4 more
semanticscholar +1 more source
Precise genome-wide mapping of single nucleosomes and linkers in vivo
Genome Biology, 2018 We developed a chemical cleavage method that releases single nucleosome dyad-containing fragments, allowing us to precisely map both single nucleosomes and linkers with high accuracy genome-wide in yeast.
Răzvan V. Chereji +3 more
semanticscholar +1 more source
Novel mechanistic insights into the role of Mer2 as the keystone of meiotic DNA break formation
eLife, 2021 In meiosis, DNA double-strand break (DSB) formation by Spo11 initiates recombination and enables chromosome segregation. Numerous factors are required for Spo11 activity, and couple the DSB machinery to the development of a meiosis-specific ‘axis ...
Dorota Rousová +10 more
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ISRN Molecular Biology, 2012 Nucleosome positioning is not only related to genomic DNA compaction but also to other biological functions. After the chromatin is digested by micrococcal nuclease, nucleosomal (nucleosome-bound) DNA fragments can be sequenced and mapped on the genomic DNA sequence.
openaire +2 more sources
Epigenetics, 2018 Previous work in Saccharomyces cerevisiae identified three residues located in close proximity to each other on the side of the nucleosome whose integrity is required for proper association of the Spt16 component of the FACT complex across transcribed ...
Eugene Nyamugenda +8 more
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BRD4 is a Histone Acetyltransferase that Evicts Nucleosomes from Chromatin
Nature Structural &Molecular Biology, 2016 Bromodomain protein 4 (BRD4) is a chromatin-binding protein implicated in cancer and autoimmune diseases that functions as a scaffold for transcription factors at promoters and super-enhancers.
B. N. Devaiah +8 more
semanticscholar +1 more source
Proceedings of the National Academy of Sciences, 1997 Archaeacontain histones that have primary sequences in common with eukaryal nucleosome core histones and a three-dimensional structure that is essentially only the histone fold. Here we report the results of experiments that document that archaeal histones compact DNAin vivointo structures similar to the structure formed by the histone (H3+H4)2tetramer
S L, Pereira +3 more
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Epichromatin and chromomeres: a ‘fuzzy’ perspective [PDF]
Open Biology, 2018 ‘Epichromatin’, the surface of chromatin beneath the interphase nuclear envelope (NE) or at the surface of mitotic chromosomes, was discovered by immunostaining with a specific bivalent mouse monoclonal anti-nucleosome antibody (mAb PL2-6). ‘Chromomeres’,
Donald E. Olins, Ada L. Olins
doaj +1 more source