Results 31 to 40 of about 42,592 (238)
Ctf4 organizes sister replisomes and Pol α into a replication factory
eLife, 2019 The current view is that eukaryotic replisomes are independent. Here we show that Ctf4 tightly dimerizes CMG helicase, with an extensive interface involving Psf2, Cdc45, and Sld5. Interestingly, Ctf4 binds only one Pol α-primase.
Zuanning Yuan +8 more
doaj +1 more source
BMC Genomics, 2023 Background Nucleosome remodeling factors regulate the occupancy and positioning of nucleosomes genome-wide through ATP-driven DNA translocation. While many nucleosomes are consistently well-positioned, some nucleosomes and alternative nucleosome ...
David C. Klein +3 more
doaj +1 more source
Nucleosome packaging and nucleosome positioning of genomic DNA [PDF]
Proceedings of the National Academy of Sciences, 1997 The goals of this study were to assess the extent to which bulk genomic DNA sequences contribute to their own packaging in nucleosomes and to reveal the relationship between nucleosome packaging and positioning. Using a competitive nucleosome reconstitution assay, we found that at least 95% of bulk DNA sequences have an affinity ...
P T, Lowary, J, Widom
openaire +2 more sources
The insulator binding protein CTCF positions 20 nucleosomes around its binding sites across the human genome. [PDF]
PLoS Genetics, 2008 Chromatin structure plays an important role in modulating the accessibility of genomic DNA to regulatory proteins in eukaryotic cells. We performed an integrative analysis on dozens of recent datasets generated by deep-sequencing and high-density tiling ...
Yutao Fu +3 more
doaj +1 more source
Single molecule analysis of CENP-A chromatin by high-speed atomic force microscopy
eLife, 2023 Chromatin accessibility is modulated in a variety of ways to create open and closed chromatin states, both of which are critical for eukaryotic gene regulation.
Daniël P Melters +4 more
doaj +1 more source
Electrophoresis of Positioned Nucleosomes [PDF]
Biophysical Journal, 2007 to appear in Biophys. J.
Castelnovo, Martin, Grauwin, Sébastian
openaire +4 more sources
Molecular Oncology, EarlyView.This review summarizes the transcription factors, repressive chromatin‐modifying complexes, and epigenetic mechanisms that control fetal hemoglobin repression. Notably, many regulators of γ‐globin silencing also function in transcriptional and epigenetic networks that drive cancer, highlighting opportunities to translate advances in hemoglobinopathy ...
Meigen Yu +3 more
wiley +1 more source
LPS-Challenged Macrophages Release Microvesicles Coated With Histones
Frontiers in Immunology, 2018 Histones are the protein component of nucleosomes, which are the basic packing unit of chromatin. However, histones are also found in the blood, both as components of nucleosomes leaked out from dead cells, or expelled from neutrophils in the active ...
Rohini Ravindran Nair +5 more
doaj +1 more source
A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins
Molecules, 2021 We report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers.
Roberto Fabian +5 more
doaj +1 more source
Spatial biology in cancer epigenetics
Molecular Oncology, EarlyView.Spatial epigenomics combines molecular profiling with tissue architecture to reveal how gene regulation is organized within intact tissues. In cancer, these technologies uncover the mechanisms driving tumor heterogeneity and microenvironmental interactions, opening new opportunities for biomarker discovery and precision medicine.
Eva Crespo‐García, Manel Esteller
wiley +1 more source