Contributions of DNA mechanics and trans-regulation to nucleosome positioning in Schizosaccharomyces pombe and its role in co-transcriptional splicing [PDF]
Epigenetics & ChromatinBackground Nucleosome positioning critically regulates chromatin functions, yet species-specific mechanisms remain incompletely understood. This study revisits nucleosome organization in Schizosaccharomyces pombe ( S.
Guoqing Liu +5 more
doaj +2 more sources
Predicting human nucleosome occupancy from primary sequence. [PDF]
PLoS Computational Biology, 2008 Nucleosomes are the fundamental repeating unit of chromatin and comprise the structural building blocks of the living eukaryotic genome. Micrococcal nuclease (MNase) has long been used to delineate nucleosomal organization.
Shobhit Gupta +5 more
doaj +3 more sources
Understanding nucleosome dynamics and their links to gene expression and DNA replication
Nature Reviews Molecular Cell Biology, 2017 Advances in genomics technology have provided the means to probe myriad chromatin interactions at unprecedented spatial and temporal resolution. This has led to a profound understanding of nucleosome organization within the genome, revealing that ...
B Franklin Pugh +2 more
exaly +2 more sources
The Effect of Micrococcal Nuclease Digestion on Nucleosome Positioning Data [PDF]
PLoS ONE, 2010 Eukaryotic genomes are packed into chromatin, whose basic repeating unit is the nucleosome. Nucleosome positioning is a widely researched area. A common experimental procedure to determine nucleosome positions involves the use of micrococcal nuclease ...
Ann Elizabeth Ehrenhofer-Murray +2 more
exaly +2 more sources
Structural Features of Transcription Factors Associating with Nucleosome Binding
Molecular Cell, 2019 SUMMARY Fate-changing transcription factors (TFs) scan chromatin to initiate new genetic programs during cell differentiation and reprogramming. Yet the protein structure domains that allow TFs to target nucleosomal DNA remain unexplored.
Katharine Schulz +2 more
exaly +2 more sources
The Ies6 subunit is essential for INO80-mediated nucleosome organization [PDF]
Scientific ReportsATP-dependent nucleosome remodelers of the INO80 family regulate chromatin by sliding, spacing and positioning nucleosomes. The INO80 remodeler is organized into structural modules that regulate its remodeling activity.
Ashish Kumar Singh +1 more
doaj +2 more sources
Differential nucleosome organization in human interphase and metaphase chromosomes [PDF]
Molecular Systems BiologyDNA bendability plays a critical role in stabilizing nucleosome assembly, yet its contribution to nucleosome dynamics in vivo remains poorly understood.
Keren Li +10 more
doaj +2 more sources
Structure of nucleosome-bound human PBAF complex
bioRxiv, 2022 BAF and PBAF are mammalian SWI/SNF family chromatin remodeling complexes that possess multiple histone/DNA-binding subunits and create nucleosome-depleted/free regions for transcription activation. Despite structural studies of nucleosome-bound human BAF
Li Wang +8 more
semanticscholar +1 more source
Structural Transition of the Nucleosome during Transcription Elongation
Cells, 2023 In eukaryotes, genomic DNA is tightly wrapped in chromatin. The nucleosome is a basic unit of chromatin, but acts as a barrier to transcription. To overcome this impediment, the RNA polymerase II elongation complex disassembles the nucleosome during ...
Tomoya Kujirai +3 more
doaj +1 more source
Histone H1 binding to nucleosome arrays depends on linker DNA length and trajectory
Nature Structural & Molecular Biology, 2022 Throughout the genome, nucleosomes often form regular arrays that differ in nucleosome repeat length (NRL), occupancy of linker histone H1 and transcriptional activity. Here, we report cryo-EM structures of human H1-containing tetranucleosome arrays with
Marco Dombrowski +4 more
semanticscholar +1 more source