Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
2º Webinar: “Cómic en lenguas indígenas: Kaqchikel y Quechua”. COST action CA19119 - ICOnMICs
Focusing on comics in indigenous languages, the event chaired by Prof. dr. Diana Castilleja (Vrije Universiteit Brussel, UCLouvain Saint-Louis Bruxelles), brings together authors, specialists and publishers in kaqchikel and quechua as part of the lines ...
Castilleja, Diana +1 more
core
Museum-based learning for cultural heritage: Examining primary students' awareness and perceptions. [PDF]
Erbaş AA, Akcan L, Akcan E.
europepmc +1 more source
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta +7 more
wiley +1 more source
Encouraging Clinical Trial Participation Through Qualitative Study and Behavior Change Methodology. [PDF]
Johnson AE.
europepmc +1 more source
Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells
To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai +8 more
wiley +1 more source
Culturally Adapted Cognitive Behavioural Therapy and First Nations Population: A Role for Nurses? [PDF]
Batterbee R +3 more
europepmc +1 more source
CONCEPTUAL APPROACH TO THE DEVELOPMENT OF HISTORICAL AND CULTURAL HERITAGE OF HOTELS
openaire +1 more source
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
A quantitative assessment of dialectal variation in a linguistic transition zone: spatial insights from vernacular bird names in southeastern Spain. [PDF]
Paracuellos M.
europepmc +1 more source

