Results 161 to 170 of about 386,968 (310)

High‐Dimensional Spectral Flow Cytometry Panel for Immunophenotypic Characterization of Fixed Whole Blood Samples

Cytometry Part A, EarlyView.
ABSTRACT Peripheral blood mononuclear cells (PBMCs) are commonly used for immune profiling in clinical research, but their isolation and cryopreservation present logistical challenges, particularly in settings with limited access to research facilities. To address this, we implemented a whole blood fixation protocol to preserve surface protein epitopes
Vaishnavi Parthasarathy, Alexander T. Heubeck, Tyanna Stuckey, Veronica Hernandez, Blessing Musgrove, Maia M. Bennett‐Boehm, Troy Torgerson, Peter Skene, Julian Reading   +8 more
wiley   +1 more source

CAQ Corner: Immune‐mediated complications

, 2022
Liver Transplantation, EarlyView.
Mary Thomson, John R. Lake
wiley   +1 more source

Flow cytometry‐based monitoring of chimeric antigen receptor (CAR) T cells: Reagent selection, assay design, and clinical utility

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract Accurate quantification of chimeric antigen receptor (CAR) T cells is essential for monitoring post‐infusion CART expansion and persistence and for real‐time clinical decision‐making. Multiparameter flow cytometry (MFC) enables rapid, live‐cell detection with absolute quantification and concurrent immunophenotypic characterization. This review
Jianhua Ling, Wei Wang, Sa A. Wang
wiley   +1 more source

A practical difference from the normal approach to evaluate natural killer (NK) cells and NK cell neoplasms by flow cytometry

Cytometry Part B: Clinical Cytometry, EarlyView.
Abstract Natural killer (NK) cell neoplasms span indolent to highly aggressive entities, and flow cytometric immunophenotyping is central to their detection and classification. However, distinguishing reactive from neoplastic NK cell expansions can be challenging because NK cells lack somatically recombined antigen receptors, and reactive states may ...
Aaron J. Wilk, Afshin Shameli, Erik Ames, Jonathan R. Fromm, Bartosz Grzywacz, Min Shi, Sindhu Cherian, Jean Oak   +7 more
wiley   +1 more source

Illuminating point‐of‐care diagnostics with aggregation‐induced emission biosensors

FlexMat, EarlyView.
This review highlights the application of aggregation‐induced emission luminogens (AIEgens) in point‐of‐care (POC) diagnostics for disease biomarker detection. It systematically examines a range of biosensing platforms, including lateral flow immunoassays, microfluidic chips, and electrochemiluminescence sensors, alongside their utility in detecting ...
Danni Hu, Yubing Hu, Dong Wang, Ben Zhong Tang   +3 more
wiley   +1 more source

Expression and immmunogenicity of HLA-B27 in high-transfection recipient P815: [PDF]

, 1989
Wildner, G., Kuon, W., Thureau, S. R., Weiss, Elisabeth H., Riethmüller, Gert   +4 more
core   +1 more source

Normal-immunopeptidome denominator: a safety atlas for immunotherapies targeting non-canonical and aberrantly expressed tumor antigens. [PDF]

J Immunother Cancer
Chen LZ.
europepmc   +1 more source

The use of fusion proteins to study HLA-B27-specific allorecognition [PDF]

, 1989
Wildner, G., Szöts, Hannelore, Schendel, Dolores J., Weiss, Elisabeth H., Riethmüller, Gert   +4 more
core   +1 more source

HLA antibodies in kidney transplantation

, 2009
In renal transplantation donor-recipient HLA-matching is performed considering HLA-A, B and DR "broad" antigens. Several studies had demonstrated that sensitized patients could produce antibodies against all HLA class I and Il molecules. In 350 HLA class
Elvira Poggi, Antonina Piazza, Domenico Adorno, Giuseppina Ozzella, Manuela Catalano, Daniela Caputo   +5 more
core  

Beyond Canonical Neoantigens: Emerging Technologies for Identification of Noncanonical Antigens and Implications for Personalized Cancer Vaccines. [PDF]

Cancers (Basel)
Yang Y, Kane T, Abdelzaher AT, Goedegebuure SP, Gillanders WE.   +4 more
europepmc   +1 more source