On the clinical relevance of using complete high-resolution HLA typing for an accurate interpretation of posttransplant immune-mediated graft outcomes [PDF]
Frontiers in Immunology, 2022 Complete and high-resolution (HR) HLA typing improves the accurate assessment of donor–recipient compatibility and pre-transplant donor-specific antibodies (DSA). However, the value of this information to identify de novo immune-mediated graft events and
Hrubá Petra +2 more
exaly +4 more sources
Evaluation of 19 years of international external proficiency testing for high-resolution HLA typing [PDF]
Frontiers in Genetics, 2023 The international high-resolution external proficiency testing (EPT) started in 2004 with high-resolution typing of human leucocyte antigen (HLA) class I (HLA-A,B,C) and HLA class II (HLA-DRB1, DRB345, DQB1, and DPB1) alleles, since possibilities for ...
C. E. M. Voorter +4 more
doaj +2 more sources
Benchmarking freely available HLA typing algorithms across varying genes, coverages and typing resolutions [PDF]
Frontiers in Immunology, 2022 Identifying the specific human leukocyte antigen (HLA) allele combination of an individual is crucial in organ donation, risk assessment of autoimmune and infectious diseases and cancer immunotherapy. However, due to the high genetic polymorphism in this
Nikolas Hallberg Thuesen +5 more
doaj +2 more sources
Extended genomic HLA typing identifies previously unrecognized mismatches in living kidney transplantation [PDF]
Frontiers in Immunology, 2023 IntroductionAntibody mediated rejection (ABMR) is the most common cause of long-term allograft loss in kidney transplantation (KT). Therefore, a low human leukocyte antigen (HLA) mismatch (MM) load is favorable for KT outcomes.
Claudia Lehmann +16 more
doaj +2 more sources
A Single-Center Experience on HLA Typing with 11 Loci Next Generation Sequencing in Korean Patients with Hematologic Disease [PDF]
Diagnostics, 2022 The human leukocyte antigen (HLA) system comprises the most polymorphic genes of the human genome and is famous for its potential pathological roles. To accurately type HLA genes and find HLA-matched donors, which are critical for effective hematopoietic
Namsoo Kim +3 more
doaj +2 more sources
consHLA: a next generation sequencing consensus-based HLA typing workflow [PDF]
BMC BioinformaticsBackground Human Leukocyte Antigens (HLA) play central roles in histocompatibility and immune system functions, including antigen presentation. Accurate typing of Class I and II HLA genes is crucial for transplant tissue matching, characterising ...
Rachel Bowen-James +5 more
doaj +2 more sources
HLA Typing for the Next Generation. [PDF]
PLoS ONE, 2015 Allele-level resolution data at primary HLA typing is the ideal for most histocompatibility testing laboratories. Many high-throughput molecular HLA typing approaches are unable to determine the phase of observed DNA sequence polymorphisms, leading to ...
Neema P Mayor +13 more
doaj +4 more sources
The Accuracy of Sequence-Specific Oligonucleotide and Real-Time Polymerase Chain Reaction HLA Typing in Determining the Presence of Pre-Transplant Donor-Specific Anti-HLA Antibodies and Total Eplet Mismatches for Deceased Donor Kidney Transplantation [PDF]
Frontiers in Immunology, 2022 High resolution human leukocyte antigen (HLA) typing is important in establishing eplet compatibility and the specificity of donor-specific anti-HLA antibodies (DSA).
Nicholas G. Larkins +14 more
doaj +2 more sources
The history and evolution of HLA typing external proficiency testing schemes in UK NEQAS for H&I [PDF]
Frontiers in Genetics, 2023 The UK National External Quality Assessment Service (NEQAS) provide an external proficiency testing (EPT) service for clinical laboratories. UK NEQAS for Histocompatibility and Immunogenetics (H&I) has been providing EPT schemes for over 45 years and
A. De’Ath, M. T. Rees, D. Pritchard
doaj +2 more sources
Cervical EVT isolation for non-invasive fetal HLA typing in early pregnancy is limited by purity and maternal cell contamination; a methodological comparison [PDF]
Frontiers in ImmunologyIntroductionMaternal-fetal HLA compatibility influences pregnancy outcome, including preeclampsia risk. Cervical extravillous trophoblasts (EVT) in early pregnancy provide a non-invasive source for fetal genome acquisition, potentially enabling fetal HLA
Liseanne J. van ‘t Hof +6 more
doaj +2 more sources