HLA Typing for the Next Generation. [PDF]
PLoS ONE, 2015 Allele-level resolution data at primary HLA typing is the ideal for most histocompatibility testing laboratories. Many high-throughput molecular HLA typing approaches are unable to determine the phase of observed DNA sequence polymorphisms, leading to ...
Neema P Mayor +13 more
doaj +5 more sources
Methods in Molecular Biology, 2020 HLA Typing James A. Hutchinson Links [A] https://www.eurotransplant.org/cms/index.php?page=et_manual [B] http://optn.transplant.hrsa.gov/policiesAndBylaws/policies.asp [C] http://www.ncbi.nlm.nih.gov/projects/gv/mhc/ [D] http://www.ebi.ac.uk/ipd/imgt/hla/
J. Hutchinson
semanticscholar +3 more sources
consHLA: a next generation sequencing consensus-based HLA typing workflow [PDF]
BMC BioinformaticsBackground Human Leukocyte Antigens (HLA) play central roles in histocompatibility and immune system functions, including antigen presentation. Accurate typing of Class I and II HLA genes is crucial for transplant tissue matching, characterising ...
Rachel Bowen-James +5 more
doaj +2 more sources
arcasHLA: high-resolution HLA typing from RNAseq. [PDF]
Bioinformatics, 2020 Motivation Human leukocyte antigen (HLA) locus makes up the major compatibility complex (MHC) and plays a critical role in host response to disease, including cancers and autoimmune disorders.
Orenbuch R +5 more
europepmc +2 more sources
HLA*LA-HLA typing from linearly projected graph alignments. [PDF]
Bioinformatics, 2019 Summary HLA*LA implements a new graph alignment model for human leukocyte antigen (HLA) type inference, based on the projection of linear alignments onto a variation graph. It enables accurate HLA type inference from whole-genome (99% accuracy) and whole-
Dilthey AT +10 more
europepmc +2 more sources
Performance of a multiplexed amplicon-based next-generation sequencing assay for HLA typing. [PDF]
PLoS One, 2020 Background Next-generation sequencing (NGS) has enabled efficient high-resolution typing of human leukocyte antigen (HLA) genes with minimal ambiguity.
Liu C +8 more
europepmc +2 more sources
The Accuracy of Sequence-Specific Oligonucleotide and Real-Time Polymerase Chain Reaction HLA Typing in Determining the Presence of Pre-Transplant Donor-Specific Anti-HLA Antibodies and Total Eplet Mismatches for Deceased Donor Kidney Transplantation [PDF]
Frontiers in Immunology, 2022 High resolution human leukocyte antigen (HLA) typing is important in establishing eplet compatibility and the specificity of donor-specific anti-HLA antibodies (DSA).
Nicholas G. Larkins +14 more
doaj +2 more sources
The history and evolution of HLA typing external proficiency testing schemes in UK NEQAS for H&I [PDF]
Frontiers in Genetics, 2023 The UK National External Quality Assessment Service (NEQAS) provide an external proficiency testing (EPT) service for clinical laboratories. UK NEQAS for Histocompatibility and Immunogenetics (H&I) has been providing EPT schemes for over 45 years and
A. De’Ath, M. T. Rees, D. Pritchard
doaj +2 more sources
Easy-HLA: a validated web application suite to reveal the full details of HLA typing. [PDF]
Bioinformatics, 2020 Motivation The HLA system plays a pivotal role in both clinical applications and immunology research. Typing HLA genes in patient and donor is indeed required in hematopoietic stem cell and solid-organ transplantation, and the histocompatibility complex ...
Geffard E +10 more
europepmc +2 more sources
Cervical EVT isolation for non-invasive fetal HLA typing in early pregnancy is limited by purity and maternal cell contamination; a methodological comparison [PDF]
Frontiers in ImmunologyIntroductionMaternal-fetal HLA compatibility influences pregnancy outcome, including preeclampsia risk. Cervical extravillous trophoblasts (EVT) in early pregnancy provide a non-invasive source for fetal genome acquisition, potentially enabling fetal HLA
Liseanne J. van ‘t Hof +6 more
doaj +2 more sources