Results 21 to 30 of about 25,975 (254)

Donor HLA Genotyping using Polymerase Chain Reaction- Sequence Specific Primers (PCR-SSP) as Method for Acquiring Donor Panel in Platelet Refractoriness

MCBS (Molecular and Cellular Biomedical Sciences), 2021
Background: Evaluation and identification of HLA antibodies in the recipient’s serum is of utmost importance prior to transplantation and transfusion. HLA typing is a steppingstone in proposing a donor panel. In order to obtain the HLA typing, Polymerase
Tubagus Djumhana Atmakusuma, Andhika Rachman, Ni Ken Ritchie   +2 more
doaj   +1 more source

Benchmarking the Human Leukocyte Antigen Typing Performance of Three Assays and Seven Next-Generation Sequencing-Based Algorithms

Frontiers in Immunology, 2021
With the great progress made recently in next generation sequencing (NGS) technology, sequencing accuracy and throughput have increased, while the cost for data has decreased.
Ping Liu, Minya Yao, Yu Gong, Yunjie Song, Yanan Chen, Yizhou Ye, Xiao Liu, Fugen Li, Hua Dong, Rui Meng, Hao Chen, Aiwen Zheng, Aiwen Zheng   +12 more
doaj   +1 more source

A glow of HLA typing in organ transplantation

Clinical and Translational Medicine, 2013
The transplant of organs and tissues is one of the greatest curative achievements of this century. In organ transplantation, the adaptive immunity is considered the main response exerted to the transplanted tissue, since the main goal of the immune ...
Batool Mutar Mahdi
doaj   +1 more source

Super high-resolution single-molecule sequence-based typing of HLA class I alleles in HIV-1 infected individuals in Ghana

PLoS ONE, 2022
Polymorphisms in human leukocyte antigen (HLA) class I loci are known to have a great impact on disease progression in HIV-1 infection. Prevailing HIV-1 subtypes and HLA genotype distribution are different all over the world, and the HIV-1 and host HLA ...
Nicholas I. Nii-Trebi, Saori Matsuoka, Ai Kawana-Tachikawa, Evelyn Y. Bonney, Christopher Z. Abana, Sampson B. Ofori, Taketoshi Mizutani, Aya Ishizaka, Teiichiro Shiino, Jun Ohashi, Taeko K. Naruse, Akinori Kimura, Hiroshi Kiyono, Koichi Ishikawa, William K. Ampofo, Tetsuro Matano   +15 more
doaj   +2 more sources

HLA typing in systemic sclerosis

Reumatismo, 2011
OBJECTIVE: The aim of the study was to investigate the relationship between Systemic Sclerosis (SSc) and HLA antigens, and to correlate these antigens with the clinical manifestations of the disease. MATERIALS AND METHODS: 55 patients were stratified according a) to the cutaneous involvement b) to the positivity of Scl-70 and anticentromere antibody ...
E. Favalli, F. Ingegnoli, S. Zeni, M. Faré, F. Fantini   +4 more
openaire   +5 more sources

Ultrarapid and high-resolution HLA class I typing using transposase-based nanopore sequencing applied in pharmacogenetic testing

Frontiers in Genetics, 2023
Nanopore sequencing has been examined as a method for rapid and high-resolution human leukocyte antigen (HLA) typing in recent years. We aimed to apply ultrarapid nanopore-based HLA typing for HLA class I alleles associated with drug hypersensitivity ...
Nampeung Anukul, Piroon Jenjaroenpun, Piroon Jenjaroenpun, Chonticha Sirikul, Natnicha Wankaew, Natnicha Wankaew, Pattaraporn Nimsamer, Pattaraporn Nimsamer, Ekkapong Roothumnong, Manop Pithukpakorn, Manop Pithukpakorn, Nipapan Leetrakool, Thidathip Wongsurawat, Thidathip Wongsurawat, Thidathip Wongsurawat   +14 more
doaj   +1 more source

HLA DNA Typing and Transplantation [PDF]

Immunity, 2001
PCR-based methods of HLA class I and class II typing have been developed that are simple, rapid, highly informative, and automatable and can be carried out at either intermediate or high levels of allelic resolution in a clinical diagnostic setting as well as for research studies.
Erlich, H.A., Opelz, G., Hansen, J.
openaire   +2 more sources

Impact of human leukocyte antigen mismatch between donor-recipient on acute rejection in liver transplantation using next-generation sequencing: a single-center study

Frontiers in Immunology
BackgroundThe effect of human leukocyte antigen (HLA) mismatch on acute rejection (AR) in liver transplantation (LT) is controversial. This study aimed to investigate the effect of donor-recipient mismatch at the HLA-A, -B, -C, -DRB1, -DRB3, -DRB4, -DRB5,
Genjie Lu, Yangfang Lu, Yanmin He, Wei Chen, Faming Zhu   +4 more
doaj   +1 more source

A Population‐Based Study on Childhood Aplastic Anemia—Incidence, Outcomes, and Health‐Related Quality of Life

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Childhood aplastic anemia (AA) is a rare disease, and both the disease itself and its treatment cause significant morbidity. We aimed to determine the contemporary incidence of childhood AA in Finland, to compare the clinical characteristics of AA against inherited bone marrow failure syndromes (IBMFS) and refractory cytopenia of ...
Lauri‐Matti Kulmala, Riitta Niinimäki, Tytti Pokka, Anu Huurre, Liisa Järvelä, Sauli Palmu, Tuuli Pöyhönen, Pauliina Utriainen, Henri Aarnivala   +8 more
wiley   +1 more source

Therapeutic Apheresis in Nigeria: A Multi‐Center Summary of Abstracts From the Inaugural Nigerian Society for Apheresis Scientific Meeting

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Background Therapeutic apheresis (TA) is an established treatment modality for hematologic, neurologic, and immunologic disorders, yet access remains severely limited in sub‐Saharan Africa. Donor apheresis, including platelet apheresis collection from healthy donors, represents an important complementary modality supporting blood product ...
Nosa Bazuaye, Chimezie Okwuonu, Evelyn Idam, Folasade Adelekan‐Popoola, Shakirat Gold‐Olufadi, Olufunto Olufela Kalejaiye, Abdullateef Ogala, Zinnah Aloziem, Blossom Amamchukwu, Patience Elimia, Mkpoikanabasi Obot‐Obot, Henry Mbakwe, Praises Maduagwuna, Olatunji Akinrodoye, Chiamaka Anyanwu, Benjamin Anyanwu, Adedamola Akinsiku, Oluwatoyin A. Akinsiku, Muzamil O. Hassan, Fatiu A. Arogundade, Olalekan E. Ojo, Homayemem Weli, Jesupelumi Adenihun, Aderemi Adelaja, Ebun Bamgboye, Olalekan Olatise, Ade E. Faponle, Ayoola Odeyemi, Michael Muoka, Stephen Asaolu, Qudrat Busari, Blessing Dawudu, Michael Naiyeju, Rasheed A. Balogun   +33 more
wiley   +1 more source