Analysis of insect holocentric chromosomes by atomic force microscopy [PDF]
In order to go in depth into the analysis of holocentric chromosome structure, atomic force microscopy (AFM) was applied to metaphase plates of the aphid Megoura viciae. AFM showed that aphid chromatids adhere to one another without any prominent structure detectable between them and without any evidence of chromosomal constrictions.
MANDRIOLI, Mauro, MANICARDI, Gian Carlo
openaire +2 more sources
Flow cytometry may allow microscope-independent detection of holocentric chromosomes in plants. [PDF]
AbstractTwo chromosomal structures, known as monocentric and holocentric chromosomes, have evolved in eukaryotes. Acentric fragments of monocentric chromosomes are unequally distributed to daughter cells and/or lost, while holocentric fragments are inherited normally.
Zedek F, Veselý P, Horová L, Bureš P.
europepmc +4 more sources
Holocentric chromosomes: convergent evolution, meiotic adaptations, and genomic analysis [PDF]
In most eukaryotes, the kinetochore protein complex assembles at a single locus termed the centromere to attach chromosomes to spindle microtubules. Holocentric chromosomes have the unusual property of attaching to spindle microtubules along their entire length.
Melters, Daniel P. +3 more
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Holocentromeres can consist of merely a few megabase-sized satellite arrays
The centromere is the chromosome region where microtubules attach during cell division. In contrast to monocentric chromosomes with one centromere, holocentric species usually distribute hundreds of centromere units along the entire chromatid.
Yi-Tzu Kuo +17 more
doaj +1 more source
HIM-10 is required for kinetochore structure and function on Caenorhabditis elegans holocentric chromosomes. [PDF]
Macromolecular structures called kinetochores attach and move chromosomes within the spindle during chromosome segregation. Using electron microscopy, we identified a structure on the holocentric mitotic and meiotic chromosomes of Caenorhabditis elegans ...
Albertson +33 more
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Chromosome Centromeres: Structural and Analytical Investigations with High Resolution Scanning Electron Microscopy in Combination with Focused Ion Beam Milling [PDF]
Whole mount mitotic metaphase chromosomes of different plants and animals were investigated with high resolution field emission scanning electron microscopy (FESEM) to study the ultrastructural organization of centromeres, including metacentric ...
Schroeder-Reiter, E., Wanner, Gerhard
core +1 more source
Stretching the Rules: Monocentric Chromosomes with Multiple Centromere Domains [PDF]
The centromere is a functional chromosome domain that is essential for faithful chromosome segregation during cell division and that can be reliably identified by the presence of the centromere-specific histone H3 variant CenH3.
A Kawabe +45 more
core +4 more sources
C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae) [PDF]
Different cytogenetic techniques including C-banding, base-specific fluorochromes and silver nitrate staining were used to compare the karyotypes of three species of bugs, representatives of the Neotropical genus Antiteuchus, namely A.
de Souza, Maria José, Lanzone, Cecilia
core +1 more source
Holocentric Chromosomes of Luzula elegans Are Characterized by a Longitudinal Centromere Groove, Chromosome Bending, and a Terminal Nucleolus Organizer Region [PDF]
The structure of holocentric chromosomes was analyzed in mitotic cells of Luzula elegans. Light and scanning electron microscopy observations provided evidence for the existence of a longitudinal groove along each sister chromatid.
A. Houben +53 more
core +1 more source
Epigenetic histone marks of extended meta-polycentric centromeres of Lathyrus and Pisum chromosomes
Species of the legume genera Lathyrus and Pisum possess chromosomes that exhibit a unique structure of their centromeric regions, which is clearly apparent during metaphase by the formation of extended primary constrictions which span up to a third of ...
Pavel eNeumann +5 more
doaj +1 more source

