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Homologous Recombination Using Bacterial Artificial Chromosomes
Cold Spring Harbor Protocols, 2015This protocol introduces the technique of homologous recombination in bacteria to insert a linear DNA fragment into bacterial artificial chromosomes (BACs). Homologous recombination allows the modification of large DNA molecules, in contrast with conventional restriction endonuclease-based strategies, which cleave large DNAs into numerous fragments and
Cary, Lai +2 more
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Initiation of homologous chromosome pairing during meiosis
Biochemical Society Transactions, 2006Following pre-meiotic DNA replication, homologous chromosomes must be paired and become tightly linked to ensure reductional segregation during meiosis I. Therefore initiation of homologous chromosome pairing is vital for meiosis to proceed correctly. A number of factors contribute to the initiation of homologous chromosome pairing including telomere ...
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Machine learning approach for homolog chromosome classification
International Journal of Imaging Systems and Technology, 2018AbstractAutomated analysis of human chromosomes is a necessary procedure to attain karyotyping and it is highly effective in cytology analysis to detect birth defects in metaspread chromosomes. In this, chromosomes are partitioned into “abnormal” and “normal” categories.
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Homologous pairing and chromosome dynamics in meiosis and mitosis
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 2004Pairing of homologous chromosomes is an essential feature of meiosis, acting to promote high levels of recombination and to ensure segregation of homologs. However, homologous pairing also occurs in somatic cells, most regularly in Dipterans such as Drosophila, but also to a lesser extent in other organisms, and it is not known how mitotic and meiotic ...
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Chromosome ends initiate homologous chromosome pairing during rice meiosis
Plant PhysiologyAbstract During meiotic prophase I, chromosomes undergo large-scale dynamics to allow homologous chromosome pairing, prior to which chromosome ends attach to the inner nuclear envelope and form a chromosomal bouquet. Chromosome pairing is crucial for homologous recombination and accurate chromosome segregation during meiosis.
Hanli You +11 more
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Manipulation of Homologous and Homoeologous Chromosome Recombination in Wheat
2016Given the sizes of the three genomes in wheat (A, B, and D) and a limited number of chiasmata formed in meiosis, recombination by crossing-over is a very rare event. It is also restricted to very similar homologues; the pairing homoeologous (Ph) system of wheat prevents differentiated chromosomes from pairing and crossing-over. This chapter presents an
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Chimpanzee chromosome 13 is homologous to human chromosome arm 2p
Cytogenetics and Cell Genetics, 2008N C, Sun, C R, Sun, T, Ho
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