Results 61 to 70 of about 348,739 (321)
Targeted recombination of homologous chromosomes using CRISPR‐Cas9
FEBS Open Bio, 2023 CRISPR mutagenesis is an efficient way to disrupt specific target genes in many model organisms. We previously devised a targeted CRISPR recombination method to generate intragenic recombinants of alleles in Drosophila.
Wonseok Son, Ki Wha Chung
doaj +1 more source
Dynamics and impact of homologous recombination on the evolution of Legionella pneumophila.
PLoS Genetics, 2017 Legionella pneumophila is an environmental bacterium and the causative agent of Legionnaires' disease. Previous genomic studies have shown that recombination accounts for a high proportion (>96%) of diversity within several major disease-associated ...
Sophia David +7 more
doaj +1 more source
Recovery of arrested replication forks by homologous recombination is error-prone. [PDF]
PLoS Genetics, 2012 Homologous recombination is a universal mechanism that allows repair of DNA and provides support for DNA replication. Homologous recombination is therefore a major pathway that suppresses non-homology-mediated genome instability.
Ismail Iraqui +6 more
doaj +1 more source
EXOSC10 is required for RPA assembly and controlled DNA end resection at DNA double-strand breaks [PDF]
, 2019 The exosome is a ribonucleolytic complex that plays important roles in RNA metabolism. Here we show that the exosome is necessary for the repair of DNA double-strand breaks (DSBs) in human cells and that RNA clearance is an essential step in homologous ...
Bonath, Franziska +7 more
core +2 more sources
Structural basis of homologous recombination [PDF]
Cellular and Molecular Life Sciences, 2019 AbstractHomologous recombination (HR) is a pathway to faithfully repair DNA double-strand breaks (DSBs). At the core of this pathway is a DNA recombinase, which, as a nucleoprotein filament on ssDNA, pairs with homologous DNA as a template to repair the damaged site.
Sun, Yueru +3 more
openaire +2 more sources
Molecular Oncology, EarlyView.Genetic testing in epithelial ovarian cancer includes both germline and tumor‐testing. This approach often duplicates resources. The current prospective study assessed the feasibility of tumor‐first multigene testing by comparing tumor tissue with germline testing of peripheral blood using an 18‐gene NGS panel in 106 patients.
Elisabeth Spenard +12 more
wiley +1 more source
VirusesThe Reverse Transcriptase of the Human Immunodeficiency Virus (HIV) is distinguished by its high rate of homologous recombination. A less-studied consequence of this phenomenon is the increased occurrence of non-homologous recombination, which results in
Arun Panchapakesan, Udaykumar Ranga
doaj +1 more source
F1000Research, 2018 Loss of genome stability is one of the hallmarks of the enabling characteristics of cancer development. Homologous recombination is a DNA repair process that often breaks down as a prelude to developing cancer. Conversely, homologous recombination can be
Nick LL van der Zon +2 more
doaj +1 more source
PCR artifact in testing for homologous recombination in genomic editing in zebrafish. [PDF]
PLoS ONE, 2017 We report a PCR-induced artifact in testing for homologous recombination in zebrafish. We attempted to replace the lnx2a gene with a donor cassette, mediated by a TALEN induced double stranded cut.
Minho Won, Igor B Dawid
doaj +1 more source
Role of the Schizosaccharomyces pombe F-box DNA helicase in processing recombination intermediates. [PDF]
, 2005 In an effort to identify novel genes involved in recombination repair, we isolated fission yeast Schizosaccharomyces pombe mutants sensitive to methyl methanesulfonate (MMS) and a synthetic lethal with rad2.
Carr, Antony M +6 more
core +2 more sources