Results 291 to 300 of about 9,526,153 (337)

Isolation and Differentiation of Human Cardiomyocyte Progenitor Cells into Cardiomyocytes

2012
To date, there is no suitable in vitro model to study human adult cardiac cell biology. Here, we describe a method for efficient isolation and expansion of human cardiomyocyte progenitor cells (CMPCs) from cardiac surgical waste or, alternatively, from fetal heart tissue.
Anke M, Smits, Angelique A, van Oorschot, Marie-José, Goumans   +2 more
openaire   +4 more sources

The human adult cardiomyocyte phenotype

Cardiovascular Research, 2003
Determination of the phenotype of adult human atrial and ventricular myocytes based on gene expression and morphology.Atrial and ventricular cardiomyocytes were obtained from patients undergoing cardiac surgery using a modified isolation procedure. Myocytes were isolated and cultured with or without serum.
Bird, SD, Doevendans, PA, van Rooijen, MA, de la Riviere, AB, Hassink, RJ, Passier, R, Mummery, CL   +6 more
openaire   +3 more sources

Adult human cardiomyocyte mechanics in osteogenesis imperfecta

American Journal of Physiology-Heart and Circulatory Physiology, 2023
In a rare donation of a heart from an individual with osteogenesis imperfecta (OI), we explored cardiomyocyte (CM) adaptations to low stiffness. This represents the first assessment of cardiomyocyte mechanics in OI. The data reveal the hypercontractility of OI CMs with rapid rundown when exposed to acute stiffness challenges, extending our ...
Benjamin W. Lee, Matthew A. Caporizzo, Christina Y. Chen, Kenneth C. Bedi, Eliot G. Peyster, Benjamin L. Prosser, Kenneth B. Margulies, Alexia Vite   +7 more
openaire   +4 more sources

The Circular RNA CDR1as Regulates the Proliferation and Apoptosis of Human Cardiomyocytes Through the miR-135a/HMOX1 and miR-135b/HMOX1 Axes

Genetic Testing and Molecular Biomarkers, 2020
Background: Chronic heart failure (CHF) is a major health burden worldwide, but there are a lack of effective methods for its early diagnosis and prognostic evaluation.
Cheng Chen, Hua Shen, Qiuping Huang, Qing Li   +3 more
semanticscholar   +1 more source

Assessment of mitophagy in human iPSC-derived cardiomyocytes

Autophagy, 2022
Defective mitophagy contributes to normal aging and various neurodegenerative and cardiovascular diseases. The newly developed methodologies to visualize and quantify mitophagy allow for additional progress in defining the pathophysiological significance of mitophagy in various model organisms. However, current knowledge regarding mitophagy relevant to
Mingchong, Yang, Ji-Dong, Fu, Jizhong, Zou, Divya, Sridharan, Ming-Tao, Zhao, Harpreet, Singh, Judith, Krigman, Mahmood, Khan, Gang, Xin, Nuo, Sun   +9 more
openaire   +2 more sources

Human cardiomyocytes undergo enhanced maturation in embryonic stem cell-derived organoid transplants.

Biomaterials, 2019
Human cardiomyocytes (CM) differentiated from pluripotent stem cells (PSCs) are relatively immature when generated in two-dimensional (2D) in vitro cultures, which limits their biomedical applications. Here, we devised a strategy to enhance maturation of
Fahimeh Varzideh, S. Pahlavan, H. Ansari, Majid Halvaei, S. Kostin, M. Feiz, H. Latifi, N. Aghdami, T. Braun, H. Baharvand   +9 more
semanticscholar   +1 more source

Human cardiomyocyte progenitor cell-derived cardiomyocytes display a maturated electrical phenotype

Journal of Molecular and Cellular Cardiology, 2010
Cardiomyocyte progenitor cells (CMPCs) can be isolated from the human heart and differentiated into cardiomyocytes in vitro. A comprehensive assessment of their electrical phenotype upon differentiation is essential to predict potential future applications of this cell source.
Boer, T.P. de, Veen, T.A.B. van, Jonsson, M.K.B., Kok, B.G.J.M., Metz, C.H.G., Sluijter, J.P.G., Doevendans, P.A., Bakker, J.M.T. de, Goumans, M.J., Heyden, M.A.G. van der   +9 more
openaire   +4 more sources

Cytotoxicity induced by fine particulate matter (PM2.5) via mitochondria-mediated apoptosis pathway in human cardiomyocytes.

Ecotoxicology and Environmental Safety, 2018
Although the strongly causal associations were between fine particulate matter (PM2.5) and cardiovascular disease, the toxic effect and potential mechanism of PM2.5 on heart was poorly understood.
Xiaozhe Yang, Lin Feng, Yannan Zhang, Hejing Hu, Yanfeng Shi, Shuang Liang, Tong Zhao, Yang Fu, Junchao Duan, Zhiwei Sun   +9 more
semanticscholar   +1 more source

Patterning the heart, a template for human cardiomyocyte development

Developmental Dynamics, 2006
AbstractAlthough in mice, the dynamics of gene expression during heart development is well characterized, information on humans is scarce due to the limited availability of material. Here, we analyzed the transcriptional distribution of Mlc‐2a, Mlc‐1v, Mlc‐2v, and atrial natriuretic factor (ANF) in human embryonic hearts between 7 and 18 weeks of ...
Susana M, Chuva de Sousa Lopes, Rutger J, Hassink, Alie, Feijen, Marga A, van Rooijen, Pieter A, Doevendans, Leon, Tertoolen, Aart, Brutel de la Rivière, Christine L, Mummery   +7 more
openaire   +2 more sources

Development of Cardiomyocytes from Human ES Cells

2003
Publisher Summary Cell transplantation is emerging as a novel strategy for myocardial regeneration, but it has been hampered by the lack of a source for human cardiomyocytes. Similarly, the lack of a human cardiomyocyte cell line has significantly limited a variety of experimental procedures.
Izhak, Kehat, Michal, Amit, Amira, Gepstein, Irit, Huber, Joseph, Itskovitz-Eldor, Lior, Gepstein   +5 more
openaire   +2 more sources