Results 1 to 10 of about 13,063 (149)

Two Clonal Cell Lines of Immortalized Human Corneal Endothelial Cells Show either Differentiated or Precursor Cell Characteristics [PDF]

Cells Tissues Organs, 2008
Access to primary human corneal endothelial cells (HCEC) is limited and donor-derived differences between cultures exacerbate the issue of data reproducibility, whereas cell lines can provide sufficient numbers of homogenous cells for multiple experiments. An immortalized HCEC population was adapted to serum-free culture medium and repeated cloning was
Valtink, Monika, Gruschwitz, Rita, Funk, Richard H. W., Engelmann, Katrin   +3 more
openaire   +5 more sources

Distribution of Precursors in Human Corneal Stromal Cells and Endothelial Cells

Ophthalmology, 2007
We identified original tissue-committed precursors with limited self-renewal capacity from human corneal stromal (HCS) cells and human corneal endothelial (HCE) cells, then tried to determine the distribution and proliferative capacity of the precursors.Experimental study.Eighteen human corneas from donors 56 to 68 years old.Human corneal stromal cells
Satoru, Yamagami, Seiichi, Yokoo, Tatsuya, Mimura, Tsuyoshi, Takato, Makoto, Araie, Shiro, Amano   +5 more
openaire   +4 more sources

Corneal Endothelial Tissue Bioengineering Using Cultured Human Corneal Endothelial Precursor Cells

, 2013
The cornea is composed of a multilayered epithelium, Bowman's membrane, stroma, Descemet's membrane, and endothelium. The corneal endothelium (CE) is a single layer of hexagonal cells that separates the corneal stroma from the aqueous humor of the anterior chamber.
Tatsuya Mimura, Seiichi Yokoo, Satoru Yamagami   +2 more
openaire   +3 more sources

Human Corneal Endothelial Cell Precursors Isolated by Sphere-Forming Assay

Investigative Opthalmology & Visual Science, 2005
To isolate precursors of human corneal endothelial cells (HCECs) in vitro.HCECs were subjected to a sphere-forming assay in which spheres floated in serum-free medium containing growth factors. To promote differentiation, the isolated sphere colonies were plated in dishes coated with poly-L-lysine (PLL)/laminin or fetal bovine endothelium extracellular
Seiichi, Yokoo, Satoru, Yamagami, Yasuo, Yanagi, Saiko, Uchida, Tatsuya, Mimura, Tomohiko, Usui, Shiro, Amano   +6 more
openaire   +3 more sources

Topical rosiglitazone is an effective anti-scarring agent in the cornea [PDF]

, 2013
Corneal scarring remains a major cause of blindness world-wide, with limited treatment options, all of which have side-effects. Here, we tested the hypothesis that topical application of Rosiglitazone, a Thiazolidinedione and ligand of peroxisome ...
Bühren, Jens, Ciufo, David, DeMagistris, Margaret, Hindman, Holly B., Huxlin, Krystel R., Jeon, Kye-Im, MacRae, Scott, Phipps, Richard P., Sime, Patricia J.   +8 more
core   +9 more sources

Potential Role of Induced Pluripotent Stem Cells (IPSCs) for Cell-Based Therapy of the Ocular Surface [PDF]

, 2015
The integrity and normal function of the corneal epithelium are crucial for maintaining the cornea's transparency and vision. The existence of a cell population with progenitor characteristics in the limbus maintains a dynamic of constant epithelial ...
Casaroli Marano, Ricardo Pedro, Edel, Michael John, Martínez-Conesa, Eva M., Nieto-Nicolau, Nuria, Álvarez Palomo, Ana Belén   +4 more
core   +6 more sources

Identification of Prdm genes in human corneal endothelium [PDF]

, 2017
Corneal endothelial cells (CECs) are essential for maintaining corneal stromal hydration and ensuring its transparency, which is necessary for normal vision. Dysfunction of CECs leads to stromal decompensation, loss of transparency and corneal blindness.
Aldahmesh, Alexandra Chittka, Arndt, Bikoff, Burkitt Wright, Chi, Chittka, Chittka, Chittka, Christiana Georgiou, Derunes, Di Zazzo, Dominic G. O'Donovan, Eghrari, Eppert, Fog, Günesdogan, Hara Susumu, Harper, Hohenauer, Juneja, Jung, Kajimura, Komai, Kostadin Rolev, Liu, Madhavan S. Rajan, Micheal, Micheal, Ohinata, Parekh, Porter, Porter, Qi, Savitsky, Seale, Siegel, Tan, Wu, Zannino, Zhang, Zhang   +41 more
core   +2 more sources

Successful transportation of human corneal endothelial tissues without cool preservation in varying Indian tropical climatic conditions and in vitro cell expansion using a novel polymer

Indian Journal of Ophthalmology, 2014
Background: Though the transplantation of human corneal endothelial tissue (CET) separated from cadaver cornea is in practice, its transportation has not been reported.
Srinivas K Rao, John Sudhakar, Periyasamy Parikumar, Sundaram Natarajan, Aditya Insaan, Hiroshi Yoshioka, Yuichi Mori, Shigeo Tsukahara, Subramani Baskar, Sadananda Rao Manjunath, Rajappa Senthilkumar, Paramasivam Thamaraikannan, Thangavelu Srinivasan, Senthilkumar Preethy, Samuel J K Abraham   +14 more
doaj   +1 more source

Construction of 3D in vitro models by bioprinting human pluripotent stem cells: Challenges and opportunities [PDF]

, 2019
Three-dimensional (3D) printing of biological material, or 3D bioprinting, is a rapidly expanding field with interesting applications in tissue engineering and regenerative medicine. Bioprinters use cells and biocompatible materials as an ink (bioink) to
Rosa, Alessandro, Salaris, Federico
core   +1 more source

Evaluation of periosteal fixation of lateral rectus and partial VRT for cases of exotropic Duane retraction syndrome

Indian Journal of Ophthalmology, 2014
Purpose: The purpose of this study is to evaluate the lateral rectus periosteal fixation and partial vertical rectus transpositioning (VRT) as treatment modalities to correct exotropic Duane retraction syndrome (Exo-DRS).
Pradeep Sharma, Ruchi Tomer, Vimla Menon, Rohit Saxena, Anudeepa Sharma   +4 more
doaj   +1 more source