Results 241 to 250 of about 466,085 (286)
Some of the next articles are maybe not open access.
Best Practice & Research Clinical Obstetrics & Gynaecology, 2016
The establishment of permanent human embryonic stem cell lines (hESCs) was first reported in 1998. Due to their pluripotent nature and ability to differentiate to all cell types in the body, they have been considered as a cell source for regenerative medicine. Since then, intensive studies have been carried out regarding factors regulating pluripotency
Pauliina, Damdimopoulou +5 more
openaire +3 more sources
The establishment of permanent human embryonic stem cell lines (hESCs) was first reported in 1998. Due to their pluripotent nature and ability to differentiate to all cell types in the body, they have been considered as a cell source for regenerative medicine. Since then, intensive studies have been carried out regarding factors regulating pluripotency
Pauliina, Damdimopoulou +5 more
openaire +3 more sources
Journal of Cell Science, 2000
ABSTRACT Embryonic stem (ES) cells are cells derived from the early embryo that can be propagated indefinitely in the primitive undifferentiated state while remaining pluripotent; they share these properties with embryonic germ (EG) cells. Candidate ES and EG cell lines from the human blastocyst and embryonic gonad can differentiate into
M F, Pera, B, Reubinoff, A, Trounson
openaire +2 more sources
ABSTRACT Embryonic stem (ES) cells are cells derived from the early embryo that can be propagated indefinitely in the primitive undifferentiated state while remaining pluripotent; they share these properties with embryonic germ (EG) cells. Candidate ES and EG cell lines from the human blastocyst and embryonic gonad can differentiate into
M F, Pera, B, Reubinoff, A, Trounson
openaire +2 more sources
Nucleofection of Human Embryonic Stem Cells
Stem Cells and Development, 2005Human embryonic stem (hES) cells provide an important tool for the study of human development, disease, and tissue regeneration. Technologies for efficient genetic modification are required to exploit hES cells fully for these applications. Here we present a customized protocol for the transfection of hES cells with the Nucleofector technology and ...
Henrike, Siemen +5 more
openaire +2 more sources
2006
Human embryonic stem cells hold great promise in furthering our treatment of disease and increasing our understanding of early development. This chapter describes protocols for the derivation and maintenance of human embryonic stem cells. In addition, it summarizes briefly several alternative methods for the culture of human embryonic stem cells. Thus,
Hidenori, Akutsu +2 more
openaire +2 more sources
Human embryonic stem cells hold great promise in furthering our treatment of disease and increasing our understanding of early development. This chapter describes protocols for the derivation and maintenance of human embryonic stem cells. In addition, it summarizes briefly several alternative methods for the culture of human embryonic stem cells. Thus,
Hidenori, Akutsu +2 more
openaire +2 more sources
The cryopreservation of human embryonic stem cells
Biotechnology and Applied Biochemistry, 2005hES (human embryonic stem) cells hold tremendous potential in the newly emerging field of regenerative medicine, in addition to being a useful tool in basic scientific research and for pharmacological and cytotoxicity screening. However, an essential prerequisite for the future widespread application of hES cells are the development of efficient ...
Boon Chin, Heng +4 more
openaire +2 more sources
Generation of Human Embryonic Stem Cells
Current Protocols in Stem Cell Biology, 2011AbstractThis unit describes generation of human embryonic stem cell lines from early human embryos. The focus is on actual handling of embryos and early embryonic outgrowths, omitting steps required for actual generation, freezing, and thawing of embryos, as well as further culture and characterization of newly derived stem cells.
Teija, Peura +3 more
openaire +2 more sources
Immunogenicity of human embryonic stem cells
Cell and Tissue Research, 2007Human embryonic stem cells (HESC) are pluripotent stem cells isolated from the inner cell mass of human blastocysts. With the first successful culturing of HESC, a new era of regenerative medicine was born. HESC can differentiate into almost any cell type and, in the future, might replace solid organ transplantation and even be used to treat ...
Karl-Henrik, Grinnemo +4 more
openaire +2 more sources
Human Embryonic Stem Cell Technology
Seminars in Reproductive Medicine, 2000Undifferentiated human embryonic stem (ES) cells can be cultured indefinitely and yet maintain the potential to form almost every cell in the adult human body. Therefore ES cells provide a model for understanding the differentiation and function of human tissue, offer new strategies for drug discovery and testing, and have the potential to provide new ...
J M, Jones, J A, Thomson
openaire +2 more sources
Human Embryonic Stem Cell Stability
Stem Cell Reviews, 2005Human embryonic stem cells (hESCs) are derived from human preimplantation embryos, and exhibit the defining characteristics of immortality and pluripotency. Indeed, these cell populations can be maintained for several years in continuous culture, and undergo hundreds of population doublings. hESCs are thus likely candidates for source of cells for cell
Lisa M, Hoffman, Melissa K, Carpenter
openaire +2 more sources
Human embryonic stem cells for cardiomyogenesis
Journal of Molecular and Cellular Cardiology, 2008Myocardial cell replacement strategies are emerging as novel therapeutic paradigms for heart failure but are hampered by the paucity of sources for human cardiomyocytes. Human embryonic stem cells (hESC) are pluripotent stem cell lines derived from human blastocysts that can be propagated, in culture, in the undifferentiated state under special ...
Manhal, Habib +2 more
openaire +2 more sources