Results 101 to 110 of about 216,145 (304)

Investigating the functionality of an OCT4-short response element in human induced pluripotent stem cells. [PDF]

, 2016
Pluripotent stem cells offer great therapeutic promise for personalized treatment platforms for numerous injuries, disorders, and diseases. Octamer-binding transcription factor 4 (OCT4) is a key regulatory gene maintaining pluripotency and self-renewal ...
Awe, Jason P, Byrne, James A, Chang, Katherine M, Hermann, Kip J, Lee, Patrick C, Lipshutz, Gerald S, Schoenberg, Benjamen E, Truong, Brian, Vega-Crespo, Agustin, Wu, Lily   +9 more
core   +3 more sources

Phosphoproteomics identifies a bimodal EPHA2 receptor switch that promotes embryonic stem cell differentiation [PDF]

, 2020
Embryonic Stem Cell (ESC) differentiation requires complex cell signalling network dynamics, although the key molecular events remain poorly understood.
Budzyk, Manon, Bustos, Francisco, Fernandez-Alonso, Rosalia, Findlay, Greg M., Helbig, Andreas O., Hukelmann, Jens, Kumar, Pankaj, Lamond, Angus I., Lanner, Fredrik, Petsalaki, Evangelia, Zhou, Houjiang   +10 more
core   +2 more sources

Tissue Engineered Human Elastic Cartilage From Primary Auricular Chondrocytes for Ear Reconstruction

Advanced Functional Materials, EarlyView.
Despite over three decades of research, no tissue‐engineered solution for auricular reconstruction in microtia patients has reached clinical translation. The key challenge lies in generating functional elastic cartilage ex vivo. Here, we integrate synergistic cell‐biomaterial strategies to engineer auricular grafts with mechanical and histological ...
Philipp Fisch, Sandra Kessler, Simone Ponta, Anna Puiggalí‐Jou, Guoliang Lyu, Killian Flégeau, Anastasiya Martyts, Florian Roth, David Fercher, Filippo M. Rijli, Daniel Simmen, Eva Novoa Olivares, Thomas Linder, Marcy Zenobi‐Wong   +13 more
wiley   +1 more source

Cell surface markers for the identification and study of human naive pluripotent stem cells

Stem Cell Research, 2018
Characterisation of mouse pluripotent stem cells has revealed two distinct pluripotent states, naive and primed, that maintain characteristics of the pre and post implanted epiblast respectively. Recent studies have developed several culture systems that
Oliver Trusler, Ziyi Huang, Jacob Goodwin, Andrew L. Laslett   +3 more
doaj   +1 more source

Microenvironmental Reprogramming by 3D Anisotropic Cardiac Extracellular Matrix Induces Nuclear Remodeling and Epigenetic Maturation of Chemically Induced Cardiomyocytes

Advanced Functional Materials, EarlyView.
A 3D anisotropic hydrogel derived from heart extracellular matrix guides cytoskeletal alignment and nuclear remodeling in reprogrammed cardiomyocyte‐like cells. This study reveals how matrix alignment modulates nuclear envelope dynamics and chromatin state, triggering transcriptional and functional maturation.
Seung Ju Seo, Mi Jeong Lee, Hyun Wook Kang, Seonhee Byeon, Soo‐Kyoung Choi, Nakwon Choi, Seung‐Woo Cho, Yoonhee Jin   +7 more
wiley   +1 more source

Assembly of Cell‐Seeded 3D Printed Hydrogel Modules with Perfusable Channel Networks

Advanced Functional Materials, EarlyView.
Macroscale assembly was utilized to prepare perfusable tissue constructs from individually 3D printed hydrogel modules with embedded branched channel networks and port arrays for cell seeding. Novel multi‐material bioreactors were fabricated to facilitate the gluing of individual modules and the perfusion culture of assembled modular constructs seeded ...
Zachary J. Geffert, Daniel Fougnier, Bo Van Durme, Jenna Grutzmacher, Ryan Clarke, Susan Cao, Zhenghao Li, Ujjwal Aryal, Jason A. Horton, Sandra Van Vlierberghe, Pranav Soman   +10 more
wiley   +1 more source

mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells

Scientific Reports, 2019
The potential applications of human pluripotent stem cells, embryonic stem (ES) cells, and induced pluripotent stem (iPS) cells in cell therapy and regenerative medicine have been widely studied.
Shuuji Mawaribuchi, Yasuhiko Aiki, Nozomi Ikeda, Yuzuru Ito   +3 more
doaj   +1 more source

Generation of Lung Epithelium from Pluripotent Stem Cells [PDF]

, 2013
The understanding of key processes and signaling mechanisms in lung development has been mainly demonstrated through gain and loss of function studies in mice, while human lung development remains largely unexplored due to inaccessibility. Several recent
Amy P. Wong, Janet Rossant
core   +1 more source

Mechanical and Electrical Phenotype of hiPSC‐Cardiomyocytes on Fibronectin‐Based Hydrogels

Advanced Healthcare Materials, EarlyView.
We introduce fibronectin‐based PEG hydrogels with controlled rigidity to enable the culture of iPSC‐derived cardiomyocytes. These substrates offer an alternative to the current culture of these cells on fibronectin‐coated glass, providing enhanced structural and functional behavior. The system provides a more physiologically relevant platform to assess
Ana Da Silva Costa, Lineta Stonkute, Sara Trujillo, Mariana Azevedo Gonzalez Oliva, Francis Burton, Matthew J. Dalby, Oana Dobre, Godfrey Smith, Manuel Salmeron‐Sanchez   +8 more
wiley   +1 more source

Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering

Cell Reports, 2018
Summary: Generating human skeletal muscle models is instrumental for investigating muscle pathology and therapy. Here, we report the generation of three-dimensional (3D) artificial skeletal muscle tissue from human pluripotent stem cells, including ...
Sara Martina Maffioletti, Shilpita Sarcar, Alexander B.H. Henderson, Ingra Mannhardt, Luca Pinton, Louise Anne Moyle, Heather Steele-Stallard, Ornella Cappellari, Kim E. Wells, Giulia Ferrari, Jamie S. Mitchell, Giulia E. Tyzack, Vassilios N. Kotiadis, Moustafa Khedr, Martina Ragazzi, Weixin Wang, Michael R. Duchen, Rickie Patani, Peter S. Zammit, Dominic J. Wells, Thomas Eschenhagen, Francesco Saverio Tedesco   +21 more
doaj   +1 more source