Results 61 to 70 of about 9,322,993 (252)

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Evaluation of CNN-based algorithms for human pose analysis of persons in red carpet scenarios

, 2017
We evaluate two CNN-based algorithms for keypoint-based human pose analysis on two image test sets containing red carpet scenarios, one taken under controlled conditions in a TV studio environment and another more heterogeneous data set taken from FlickR
Richter, Daniel, Brunnett, Guido, Heinzig, Manuel, Kowerko, Danny, Kahl, Stefan, Helmert, Stefan   +5 more
core   +1 more source

Neural Network Approach for 2-Dimension Person Pose Estimation With Encoded Mask and Keypoint Detection

IEEE Access, 2020
We present an innovative approach for 2D person pose estimation by developing a convolutional neural network for human 2-channel mask prediction and human 2D pose estimation.
Tahir Rizwan, Yunze Cai, Muhammad Ahsan, Noman Sohail, Emad Abouel Nasr, Haitham A. Mahmoud   +5 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Deep Models for Rigid Objects Real-Time Pose Estimation [PDF]

Accurate and robust six degrees of freedom (6-DoF) pose estimation of rigid objects is one of the fundamental tasks in computer vision, with wide-ranging applications that span industrial automation, augmented reality, and medical intervention.
Zhao, Jianyu
core   +1 more source

THANet: Transferring Human Pose Estimation to Animal Pose Estimation

, 2023
Animal pose estimation (APE) boosts the understanding of animal behaviors. Recent vision-based APE has attracted extensive attention due to the advantages of contactless and sensorless applications.
Jincheng Liao, Shaohui Lin, Yunhang Shen, Jianzhong Xu   +3 more
core   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

A Multi-view Pixel-wise Voting Network for 6DoF Pose Estimation [PDF]

, 2022
open6DoF pose estimation is an important task in the Computer Vision field for what regards robotic and automotive applications. Many recent approaches successfully perform pose estimation on monocular images, which lack depth information.
DONADI, IVANO
core  

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source