Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Utility of Ischemia Modified Albumin as an Early Marker for Diagnosis of Acute Coronary Syndrome
Journal of Nepal Health Research Council, 2018 Background: The diagnosis of acute coronary syndrome remains challenging, as cardiac troponins and creatine kinase-MB do not detect myocardial ischemia.
Bijaya Mishra +6 more
doaj +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
PENGARUH PEMBERIAN SUPLEMENTASI SUPEROXIDE DISMUTASE (SOD) TERHADAP KADAR ALBUMIN SERUM PADA LANSIA [PDF]
, 2015 Background: Levels of serum albumin can be used as a predictor of morbidity and mortality in the elderly. Reduced serum albumin concentration can be caused by oxidative modification due to aging or insufficient protein intake.
Nalaresi, Andyta +2 more
core
The binding of dyestuffs by proteins [PDF]
, 1950 Thesis (M.A.)--Boston ...
Scully, Margaret A
core +1 more source
Encapsulation of human serum albumin in submicrometer magnetic poly(lactide-co-glycolide) particles as a model system for targeted drug delivery [PDF]
, 2013 Two types of iron oxide nanoparticles were synthesized by coprecipitation of Fe(II) and Fe(III) chlorides: water-dispersible γ-Fe2O3 and organic solvent-dispersible oleic acid-coated Fe3O4 particles.
Feczkó, Tivadar +6 more
core +1 more source
Translational and rotational motions of albumin sensed by a non-covalent associated porphyrin under physiological and acidic conditions: a fluorescence correlation spectroscopy and time resolved anisotropy study. [PDF]
, 2008 The interaction between a free-base, anionic water-soluble porphyrin, TSPP, and the drug carrier protein, bovine serum albumin (BSA) has been studied by time-resolved fluorescence anisotropy (TRFA) and fluorescence correlation spectroscopy (FCS) at two ...
Andrade, S.M. +4 more
core +2 more sources
Chemical analysis of soluble fractions from normal and autolysed rabbit liver by column chromatography [PDF]
, 1966 Chromatography on Sephadex G-200 was performed with the soluble fraction of homogenated rabbit liver, which was extracted with 0.1 M phosphate buffer containing 0.1 M NaCl.
Aizawa, Tadashi +4 more
core +1 more source
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective To (1) validate GAD65‐ELISA detection and quantification for type 1 diabetes mellitus and autoimmune neurological diagnoses, (2) correlate ELISA results (reference range < 5 IU/mL) with established radioimmunoprecipitation assay (RIA; ≤ 0.02 nmol/L), and (3) define ELISA clinical utility and pitfalls.
Andrew McKeon +11 more
wiley +1 more source
POTENTIAL IMPACTS OF PHARMACEUTICAL USES OF TRANSGENIC TOBACCO: THE CASE OF HUMAN SERUM ALBUMIN (HSA) [PDF]
The potential size and distribution of benefits from transgenic tobacco as a source of human serum albumin are estimated using an economic surplus model with imperfect competition.
Kostandini, Genti +2 more
core +1 more source