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Generation of a <i>scFv</i> Derived from an IgM-Producing Hybridoma for the Detection of REST Expression in Premalignant Lesions and Invasive Squamous Cell Carcinoma. [PDF]
Rodríguez-Nava C +9 more
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Development of a specific anti-human EphA3 monoclonal antibody, Ea<sub>3</sub>Mab-20, for flow cytometry. [PDF]
Satofuka H +7 more
europepmc +1 more source
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Cold Spring Harbor Protocols, 2021
This introduction discusses the techniques used to grow and maintain myeloma and hybridoma cell lines, the production and collection of monoclonal antibodies, and methods for drug selection used in hybridoma work.
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This introduction discusses the techniques used to grow and maintain myeloma and hybridoma cell lines, the production and collection of monoclonal antibodies, and methods for drug selection used in hybridoma work.
openaire +2 more sources
Science, 1980
In the report by John C. Behrendt et al. "Aeromagnetic and radio echo ice-sounding measurements show much greater area of the Dufek Intrusion, Antarctica" (29 Aug., p. 1014), the word "expedition" should have read "exploitation" in line 13 of the first paragraph on page 1014.
H, Koprowski, C, Croce
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In the report by John C. Behrendt et al. "Aeromagnetic and radio echo ice-sounding measurements show much greater area of the Dufek Intrusion, Antarctica" (29 Aug., p. 1014), the word "expedition" should have read "exploitation" in line 13 of the first paragraph on page 1014.
H, Koprowski, C, Croce
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2015
The generation of hybridoma cell lines by the fusion of splenocytes from immunized mice with immortal myeloma cells is a well-established method for the production of monoclonal antibodies. Although other methods have emerged as an effective alternative for the generation of monoclonal antibodies, the use of hybridoma technology remains a viable ...
Robert M, Hnasko, Larry H, Stanker
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The generation of hybridoma cell lines by the fusion of splenocytes from immunized mice with immortal myeloma cells is a well-established method for the production of monoclonal antibodies. Although other methods have emerged as an effective alternative for the generation of monoclonal antibodies, the use of hybridoma technology remains a viable ...
Robert M, Hnasko, Larry H, Stanker
openaire +2 more sources
2005
Hybridomas can be grown in a number of ways to produce stocks of monoclonal antibodies. Smaller volumes may be produced in static flask culture, but if larger amounts are required, a bioreactor may be used.
Gary, Entrican, Gareth, Young
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Hybridomas can be grown in a number of ways to produce stocks of monoclonal antibodies. Smaller volumes may be produced in static flask culture, but if larger amounts are required, a bioreactor may be used.
Gary, Entrican, Gareth, Young
openaire +2 more sources
Cell hybridization, hybridomas, and human hybridomas.
Methods in cell biology, 1998Cell hybridization is one of the most basic cytotechnologies. The hemagglutinating virus of Japan was first used to cause cell fusion; however, polyethylene glycol is widely used now because of simplicity of procedure. This chapter first explains the principles of cell hybridization methods and then describes the practical protocols for preparing mouse
S, Shirahata, Y, Katakura, K, Teruya
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Journal of Immunology, 1989
By establishing hybridomas from two distinct surface IgM+ splenic B cell populations, Ly-1 B cells and "conventional" (Ly-1-) B cells, we found that the Ly-1 B population includes a 30 to 70 times higher frequency (1 to 2%) of cells with specificity for ...
Richard R. Hardy +4 more
semanticscholar +1 more source
By establishing hybridomas from two distinct surface IgM+ splenic B cell populations, Ly-1 B cells and "conventional" (Ly-1-) B cells, we found that the Ly-1 B population includes a 30 to 70 times higher frequency (1 to 2%) of cells with specificity for ...
Richard R. Hardy +4 more
semanticscholar +1 more source

