Results 151 to 160 of about 1,201 (167)

Hydration in aqueous solutions of ectoine and hydroxyectoine

Physical Chemistry Chemical Physics, 2018
We explore the influence of the two osmolytes ectoine and hydroxyectoine on the structure of pure water and aqueous NaCl solutions using non-resonant X-ray Raman scattering spectroscopy at the oxygen K-edge.
Christoph J. Sahle, Martin A. Schroer, Cy M. Jeffries, Johannes Niskanen   +3 more
openaire   +3 more sources

The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum

Extremophiles, 2014
Acidiphilium cryptum is an acidophilic, heterotrophic α-Proteobacterium which thrives in acidic, metal-rich environments (e.g. acid mine drainage). Recently, an ectABCDask gene cluster for biosynthesis of the compatible solutes ectoine and hydroxyectoine was detected in the genome sequence of A. cryptum JF-5.
Katharina D, Moritz, Birgit, Amendt, Elisabeth M H J, Witt, Erwin A, Galinski   +3 more
exaly   +3 more sources

Engineering of Hydroxyectoine Production based on the Methylomicrobium alcaliphilum

Applied Biochemistry and Microbiology, 2019
Ectoine is an osmoprotector that is synthesized by many halophilic and halotolerant bacteria in response to osmotic stress. The halotolerant methanotroph Methylomicrobium alcaliphilum 20Z was genetically modified to produce ectoine derivative hydroxyectoine by introduction of the gene encoding ectoine hydroxylase EctD from Pseudomonas stutzeri DSM5190T.
I. I. Mustakhimov, A. S. Reshetnikov, S. Y. But, O. N. Rozova, V. N. Khmelenina, Y. A. Trotsenko   +5 more
exaly   +2 more sources

Ectoine and hydroxyectoine inhibit thermal-induced aggregation and increase thermostability of recombinant human interferon Alfa2b

European Journal of Pharmaceutical Sciences, 2017
This study is to investigate whether ectoines (ectoine and hydroxyectoine) can reduce aggregation of rhIFNα2b in aqueous solutions on thermal stress. The effect of thermal stress condition on the stability was therefore investigated using size exclusion-high performance liquid chromatography (SE-HPLC), different spectroscopic measurements, dynamic ...
Faranak Salmannejad, Mehdi Mogharabi-Manzari   +1 more
exaly   +3 more sources

Supplementation effects of hydroxyectoine on proline uptake of downshocked Brevibacterium sp. JCM 6894

Journal of Bioscience and Bioengineering, 2006
Downshock treatment of the halotolerant Brevibacterium sp. JCM 6894 was a prerequisite for proline uptake which is a function for cell survival. Hydroxyectoine served as an effective stimulator for the proline uptake and cell survival of the downshocked cells of this strain.
Chenxiang, Wang, Daochen, Zhu, Shinichi, Nagata   +2 more
openaire   +2 more sources

A Specialized Aspartokinase Enhances the Biosynthesis of the Osmoprotectants Ectoine and Hydroxyectoine in Pseudomonas stutzeriA1501 [PDF]

Journal of Bacteriology, 2011
ABSTRACTThe compatible solutes ectoine and hydroxyectoine are widely produced by bacteria as protectants against osmotic and temperature stress.l-Aspartate-beta-semialdehyde is used as the precursor molecule for ectoine/hydroxyectoine biosynthesis that is catalyzed by the EctABCD enzymes.l-Aspartate-beta-semialdehyde is a central intermediate in ...
Sander H J Smits, Johann Heider, Erhard Bremer   +2 more
exaly   +3 more sources

Metabolic engineering of Escherichia coli for efficient osmotic stress‐free production of compatible solute hydroxyectoine

Biotechnology and Bioengineering, 2021
AbstractCompatible solutes are key for the ability of halophilic bacteria to resist high osmotic stress. They have received wide attention from researchers for their excellent osmotic protection properties. Hydroxyectoine is a particularly important compatible solute, but its production by microbes faces several challenges, including low titer/yield ...
Qian Ma, Li Xia, Heyun Wu, Mingyang Zhuo, Mengya Yang, Ying Zhang, Miao Tan, Kexin Zhao, Quanwei Sun, Qingyang Xu, Ning Chen, Xixian Xie   +11 more
openaire   +2 more sources

High efficiency production of 5-hydroxyectoine using metabolically engineered Escherichia coli

Bioresource Technology
The 5-hydroxyectoine is a natural protective agent with long-lasting moisturising and radiation resistance properties. It can be naturally synthesized by some extremophiles using the "bacterial milking" process, but this can corrode bioreactors and downstream purification may cause environmental pollution. In this study, an engineered Escherichia coli (
Zhijie Qin, Lihong Li, Weizhu Zeng, Hongbiao Li, Jingwen Zhou, Sha Xu   +5 more
openaire   +2 more sources

Hydroxyectoine protects Mn-depleted photosystem II against photoinhibition acting as a source of electrons

Photosynthesis Research, 2019
In the present study, we have investigated the effect of hydroxyectoine (Ect-OH), a heterocyclic amino acid, on oxygen evolution in photosystem II (PS II) membrane fragments and on photoinhibition of Mn-depleted PS II (apo-WOC-PS II) preparations. The degree of photoinhibition of apo-WOC-PS II preparations was estimated by the loss of the capability of
Yanykin D. V., Malferrari M., Rapino S., Venturoli G., Semenov A. Y., Mamedov M. D.   +5 more
openaire   +3 more sources

Anhydrobiotic engineering for the endophyte bacterium Kosakonia radicincitans by osmoadaptation and providing exogenously hydroxyectoine

World Journal of Microbiology and Biotechnology, 2019
This study presents an anhydrobiotic engineering approach aiming at conferring a high degree of desiccation tolerance to the Gram-negative endophyte Kosakonia radicincitans. In particular, pre-conditioning of bacteria under high salinities provides a remarkable positive influence on drying survival.
Cruz Barrera, Mauricio, Jakobs-Schönwandt, Désirée, Persicke, Marcus, Gomez, Martha Isabel, Ruppel, Silke, Patel, Anant   +5 more
openaire   +3 more sources