Results 61 to 70 of about 1,201 (167)

Transport kinetics of ectoine, an osmolyte produced by Brevibacterium epidermis [PDF]

open access: yes, 2006
Brevibacterium epidermis DSM 20659 is a halotolerant Gram-positive bacterium which can synthesize the osmolyte, ectoine, but prefers to take it up from its environment. The present study revealed that B.
De Mey, Marjan   +4 more
core   +1 more source

Ectoine can enhance structural changes in DNA in vitro [PDF]

open access: yes, 2017
Strand breaks and conformational changes of DNA have consequences for the physiological role of DNA. The natural protecting molecule ectoine is beneficial to entire bacterial cells and biomolecules such as proteins by mitigating detrimental effects of ...
Hahn, M. B.   +5 more
core   +1 more source

With a pinch of extra salt-Did predatory protists steal genes from their food?

open access: yesPLoS Biology, 2018
The cellular adjustment of Bacteria and Archaea to high-salinity habitats is well studied and has generally been classified into one of two strategies.
Laura Czech, Erhard Bremer
doaj   +1 more source

Developing High‐Efficiency Electroporation Protocols for Hard‐To‐Transform Halomonas spp.

open access: yesMicrobial Biotechnology, Volume 18, Issue 12, December 2025.
Genetic manipulation of Halomonas species has been limited by poor transformation efficiencies. Here, we establish a highly efficient electroporation protocol for Halomonas elongata DSM 2581. This method also proved transferable to other Halomonas strains, encouraging further genetic manipulation work in underexplored species.
André A. B. Coimbra   +2 more
wiley   +1 more source

Compatible solutes from hyperthermophiles improve the quality of DNA microarrays

open access: yesBMC Biotechnology, 2007
Background DNA microarrays are among the most widely used technical platforms for DNA and RNA studies, and issues related to microarrays sensitivity and specificity are therefore of general importance in life sciences. Compatible solutes are derived from
Evangelisti Rita   +13 more
doaj   +1 more source

Production of hydroxyectoine from biogas by an engineered strain of Methylomicrobium alcaliphilum using a novel Taylor‐flow bioreactor

open access: yesJournal of Chemical Technology &Biotechnology, Volume 100, Issue 7, Page 1425-1433, July 2025.
Abstract BACKGROUND The production of compatible solutes, such as ectoine and hydroxyectoine, is of great interest due to their industrial and biotechnological applications. Methylomicrobium alcaliphilum was genetically engineered to replace a native gene with a heterologous one, aiming to enhance ectoine production.
Raquel Herrero‐Lobo   +7 more
wiley   +1 more source

High-salinity growth conditions promote tat-independent secretion of tat substrates in Bacillus subtilis [PDF]

open access: yes, 2012
The Gram-positive bacterium Bacillus subtilis contains two Tat translocases, which can facilitate transport of folded proteins across the plasma membrane. Previous research has shown that Tat-dependent protein secretion in B.
Alami   +59 more
core   +1 more source

Genome‐Scale Metabolic Modeling of Halomonas elongata 153B Explains Polyhydroxyalkanoate and Ectoine Biosynthesis in Hypersaline Environments

open access: yesBiotechnology Journal, Volume 19, Issue 10, October 2024.
Graphical Abstract and Lay Summary We constructed and validated a genome‐scale metabolic model for Halomonas elongata, revealing key metabolic changes during ectoine and PHA biosynthesis, including reduced oxidative phosphorylation and enhanced precursor production.
Blaise Manga Enuh   +2 more
wiley   +1 more source

The MtrAB two-component system controls antibiotic production in Streptomyces coelicolor A3(2 [PDF]

open access: yes, 2017
MtrAB is a highly conserved two-component system implicated in the regulation of cell division in the Actinobacteria. It coordinates DNA replication with cell division in the unicellular Mycobacterium tuberculosis and links antibiotic production to ...
Barrie Wilkinson   +14 more
core   +1 more source

Combined influence of ectoine and salt: spectroscopic and numerical evidence for compensating effects on aqueous solutions [PDF]

open access: yes, 2016
Ectoine is an important osmolyte, which allows microorganisms to survive in extreme environmental salinity. The hygroscopic effects of ectoine in pure water can be explained by a strong water binding behavior whereas a study on the effects of ectoine in ...
Hahn, Marc Benjamin   +4 more
core   +2 more sources

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