Results 211 to 220 of about 17,899 (259)
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Hypoxanthine-Guanine Phosphoribosyltransferase Deficiency in Gout
Annals of Internal Medicine, 1969Excerpt INTRODUCTION Sophisticated biochemical studies in recent years have revealed that the regulation of intracellular metabolism is a logical, orderly, and intricate process.
Martin L. Greene +4 more
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A spectrophotometric assay for hypoxanthine-guanine phosphoribosyltransferase
Analytical Biochemistry, 1971Abstract The present paper describes a new spectrophotometric assay for HGPRTase activity which is more rapid than and as sensitive as the isotopic assays for this enzyme and which avoids the use of high-voltage electrophoresis and liquid scintillation counting. A simple technique using thin-layer chromatography for the separation of the nucleotide,
David S. Newcombe, James M. Willard
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Clinical Laboratory, 2018
BACKGROUND Lesch-Nyhan syndrome (LNS) is a congenital X-linked recessive neurogenetic disorder caused by mutations in the hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene.
Jiao Huang +6 more
semanticscholar +1 more source
BACKGROUND Lesch-Nyhan syndrome (LNS) is a congenital X-linked recessive neurogenetic disorder caused by mutations in the hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene.
Jiao Huang +6 more
semanticscholar +1 more source
International Journal of Biochemistry, 1986
A rapid and simple method, based on GMP Sepharose affinity chromatography, was used for the purification of human brain hypoxanthine guanine phosphoribosyltransferase. A single protein band was detected by polyacrylamide gel electrophoresis of the native purified enzyme. A subunit molecular weight of 25,000 was estimated by SDS gel electrophoresis. The
Shigeki Nakagawa +3 more
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A rapid and simple method, based on GMP Sepharose affinity chromatography, was used for the purification of human brain hypoxanthine guanine phosphoribosyltransferase. A single protein band was detected by polyacrylamide gel electrophoresis of the native purified enzyme. A subunit molecular weight of 25,000 was estimated by SDS gel electrophoresis. The
Shigeki Nakagawa +3 more
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Rapid detection of hypoxanthine-guanine phosphoribosyltransferase on cellogel
Humangenetik, 1974A simple, fast and direct staining method for the detection of hypoxanthineguanine phosphoribosyltransferase is described. It is based on the conversion of inosine monophosphate to hypoxanthine, which is then enzymatically oxidized. This oxidation is coupled to the reduction of a tetrazolium salt to blue formazan.
Someren, H. van +2 more
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ACS Chemical Biology, 2017
Plasmodium falciparum (Pf) and Plasmodium vivax (Pv) are the foremost causative agents of malaria. Due to the development of resistance to current antimalarial medications, new drugs for this parasitic disease need to be discovered.
D. Keough +12 more
semanticscholar +1 more source
Plasmodium falciparum (Pf) and Plasmodium vivax (Pv) are the foremost causative agents of malaria. Due to the development of resistance to current antimalarial medications, new drugs for this parasitic disease need to be discovered.
D. Keough +12 more
semanticscholar +1 more source
A continuous spectrophotometric assay for hypoxanthine-guanine phosphoribosyltransferase
Analytical Biochemistry, 1977Abstract The present paper describes a simple and rapid spectrophotometric assay of hypoxanthine-guanine phosphoribosyltransferase based on the continuous monitoring of product concentration by a NADH-coupled enzyme system.
GIACOMELLO, Alessandro +1 more
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Immunoadsorbent Chromatography of Hypoxanthine-Guanine Phosphoribosyltransferase
1974Recent evidence indicates that the virtual absence of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) in patients with the Lesch-Nyhan syndrome is due in most if not all instances to a mutation(s) on the gene coding for the HGPRT protein (Kelley and Meade, 1971; Rubin, et al., 1971; Arnold, Meade and Kelley, 1972).
R. B. Jones +2 more
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Purification of hypoxanthine-guanine phosphoribosyltransferase of Plasmodium lophurae
Molecular and Biochemical Parasitology, 1987Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) was isolated from the malarial parasite, Plasmodium lophurae. The apparent pI, as determined by chromatofocusing, was 7.6. The native molecular weight was 79,000. The pH profile of HGPRT exhibited a broad pH optimum. With hypoxanthine as substrate maximal activity was achieved from pH 6.0-10.0,
Larry A. Mole +2 more
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Hypoxanthine-guanine phosphoribosyltransferase: A simple spectrophotometric assay
Clinica Chimica Acta, 1977A simple spectrophotometric assay is described based on the conversion of hypoxanthine to inosine monophosphate and precipitation of both the reaction product and protein with lanthanum phosphate. The extent of conversion is determined by the fall in absorbance of hypoxanthine at 249 nm.
Johnson L.A., Gordon R.B., Emmerson B.T.
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