Results 11 to 20 of about 34,291 (223)

Hypoxanthine-Guanine Phosphoribosyltransferase/adenylate Kinase From Zobellia galactanivorans: A Bifunctional Catalyst for the Synthesis of Nucleoside-5′-Mono-, Di- and Triphosphates [PDF]

Frontiers in Bioengineering and Biotechnology, 2020
In our search for novel biocatalysts for the synthesis of nucleic acid derivatives, we found a good candidate in a putative dual-domain hypoxanthine-guanine phosphoribosyltransferase (HGPRT)/adenylate kinase (AMPK) from Zobellia galactanivorans (ZgHGPRT ...
Javier Acosta, Jon Del Arco, Maria Luisa Del Pozo, Beliña Herrera-Tapias, Vicente Javier Clemente-Suárez, Vicente Javier Clemente-Suárez, José Berenguer, Aurelio Hidalgo, Jesús Fernández-Lucas, Jesús Fernández-Lucas   +9 more
doaj   +2 more sources

Investigation of the functional hot-spot residues of an enzyme by real-time monitoring of the enzymatic reaction using NMR and computational approaches [PDF]

Scientific Reports
Favipiravir is an anti-influenza prodrug that is metabolized to its phosphoribosylated form, favipiravir-ribofuranosyl-5′-monophosphate (favipiravir-RMP), by human endogenous enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT).
Toshihiko Sugiki, Tomoki Yoshida, Masaki Tsukamoto, Koichiro Miyanishi, Akinori Kagawa, Natsuko Miura, Tomoto Ura, Jun Fukazawa, Yuko Hatanaka, Tsuyoshi Murata, Toshimichi Fujiwara, Masahiro Kitagawa, Yasushi Morita, Kumiko Sakai-Kato, Yoichi Takakusagi, Nobutada Tanaka, Makoto Negoro   +16 more
doaj   +2 more sources

The Effect of Two Weeks of High-intensity Interval Training on Salvage nucleotide pathway [PDF]

فیزیولوژی ورزش و فعالیت بدنی, 2022
Purpose: The effects of exercise adaptation on improving the purine nucleotides components are inevitable, and physical activity improves the purine nucleotide salvage pathway.
Abbas Ghanbari Niaki, Rouhollah Haghshenas Gatabi   +1 more
doaj   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway. [PDF]

FEBS Open Bio
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Guo Y, Richter S, Bach C, Kiess W, Kirstein A, Le Duc D, Garten A.   +6 more
europepmc   +2 more sources

De novo or Salvage? Nucleotide Availability as a Driver of Bacterial Adaptation and Virulence. [PDF]

Microbiologyopen
Bacterial pathogens survive in different host environments by switching between making nucleotides by de novo synthesis and scavenging them from the host. This flexibility supports growth and virulence making nucleotide metabolism an attractive therapeutic target.
Joshi R, McEwan AG, Kappler U.
europepmc   +2 more sources

Biosynthesis and Genetic Tuning of Guanine Crystals via Metabolic Engineering of Yeast. [PDF]

Angew Chem Int Ed Engl
We demonstrate how yeast cells can be harnessed as cellular factories for the synthesis of optically functional guanine crystals, where genetic editing is used to tune crystal size, order and optical properties. This demonstrates a powerful new biosynthetic approach for the development of sustainable and biocompatible optical pigments, which utilizes ...
Janssen JN, Fishman Y, Li Y, Wagner A, Pavan MJ, Paleiov Z, Lemcoff T, Lichtenstein-Wolfheim R, Upcher A, Nativ-Roth E, Aharoni A, Palmer BA.   +11 more
europepmc   +3 more sources

Effects of Immunization with Recombinant Schistosoma mansoni Enzymes AK and HGPRT: Murine Infection Control

Pathogens, 2023
Schistosomiasis is one of the most important human helminthiases worldwide. Praziquantel is the current treatment, and no vaccine is available until the present.
Ana Carolina Maragno Fattori, Elisandra de A. Montija, Bruna D. de L. Fragelli, Ricardo de O. Correia, Cynthia Aparecida de Castro, Larissa Romanello, Camila T. Nogueira, Silmara M. Allegretti, Edson G. Soares, Humberto D. Pereira, Fernanda de F. Anibal   +10 more
doaj   +1 more source

Adeno‐associated virus serotype 2 capsid variants for improved liver‐directed gene therapy

Hepatology, EarlyView., 2022
Abstract Background and Aims Current liver‐directed gene therapies look for adeno‐associated virus (AAV) vectors with improved efficacy. With this background, capsid engineering is explored. Whereas shuffled capsid library screenings have resulted in potent liver targeting variants with one first vector in human clinical trials, modifying natural ...
Nadja Meumann, Marti Cabanes‐Creus, Moritz Ertelt, Renina Gale Navarro, Julie Lucifora, Qinggong Yuan, Karin Nien‐Huber, Ahmed Abdelrahman, Xuan‐Khang Vu, Liang Zhang, Ann‐Christin Franke, Christian Schmithals, Albrecht Piiper, Annabelle Vogt, Maria Gonzalez‐Carmona, Jochen T. Frueh, Evelyn Ullrich, Philip Meuleman, Steven R. Talbot, Margarete Odenthal, Michael Ott, Erhard Seifried, Clara T. Schoeder, Joachim Schwäble, Leszek Lisowski, Hildegard Büning   +25 more
wiley   +1 more source

Research Advances in Purine Phosphoribosyltransferases of Protozoan Parasites

Guangdong nongye kexue, 2023
Parasitic protozoa are single-celled organisms that have adapted to live in cells of humans and animals. The protozoan parasites include Leishmania spp., Trypanosoma spp., Plasmodium spp., Toxoplasma gondii, Cryptosporidium spp., and Eimeria spp., which ...
Zhihui YU, Siyun FANG, Mingfei SUN, Nanshan QI, Wenjun LIU, Juan LI, Junjing HU, Shenquan LIAO   +7 more
doaj   +1 more source

Case of Self-Mutilation –Lesch-Nyhan Syndrome

Journal of College of Medical Sciences-Nepal, 2022
Lesch-Nyhan syndrome involves a congenital error of purine metabolism, due to the absence (or very low levels) of hypoxanthine-guanine phosphoribosyltransferase (HPRT) enzyme. The classic clinical phenotype of LNS was first described by Michael Lesch and
Sweta Kumari Gupta, Samanata Dallakoti, Ranjit Sapkota, Niraj Sangroula   +3 more
doaj   +1 more source