Results 31 to 40 of about 34,291 (223)

Temporal, Spatial and Tissue-Specific Expression of a Myogenin-lacZ Transgene Targeted to the Hprt Locus in Mice

BioTechniques, 1999
A lacZ transgene, expressed by the myogenin promoter, was introduced into the mouse hypoxanthine phosphoribosyltransferase (Hprt) locus by gene targeting in embryonic stem cells.
Jay L. Vivian, William H. Klein, Paul Hasty   +2 more
doaj   +1 more source

Identification of stable reference genes for quantitative gene expression analysis in the duodenum of meat-type ducks

Frontiers in Veterinary Science, 2023
Quantitative polymerase chain reaction (qPCR) is an important method to detect gene expression at the molecular level. The selection of appropriate housekeeping genes is the key to accurately calculating the expression level of target genes and ...
Fei Shui, Fei Shui, Guiru Qiu, Shenqiang Pan, Xin Wang, Xin Wang, Fumin Jia, Fumin Jia, Tingting Jiang, Yongsheng Li, Zhaoyu Geng, Zhaoyu Geng, Sihua Jin, Sihua Jin   +13 more
doaj   +1 more source

dGTP starvation in Escherichia coli provides new insights into the thymineless-death phenomenon.

PLoS Genetics, 2014
Starvation of cells for the DNA building block dTTP is strikingly lethal (thymineless death, TLD), and this effect is observed in all organisms. The phenomenon, discovered some 60 years ago, is widely used to kill cells in anticancer therapies, but many ...
Mark Itsko, Roel M Schaaper
doaj   +1 more source

Lesch-Nyhan Disease and Its Variants: Phenotypic and Mutation Spectrum of Hypoxanthine-Guanine Phosphoribosyltransferase Deficiency in Argentine Patients

Journal of Inborn Errors of Metabolism and Screening, 2021
Hypoxanthine-guanine phosphoribosyltransferase (HPRT) deficiency is a disorder of purine metabolism responsible for Lesch-Nyhan Disease (LND) and its variants, HPRT-related hyperuricemia with neurologic dysfunction (HND) and HPRT-related hyperuricemia ...
Laura E. Laróvere, Lynette D. Fairbanks, H. A. Jinnah, Norberto B. Guelbert, Emilia Escuredo, Adriana Becerra, Raquel Dodelson de Kremer   +6 more
doaj   +1 more source

Hypoxanthine Guanine Phosphoribosyltransferase expression is negatively correlated with immune activity through its regulation of purine synthesis

, 2020
The purpose of this study was to examine the effects of elevated Hypoxanthine Guanine Phosphoribosyltransferase (HPRT) on the immune response in the tumor ...
Claudia M. Tellez Freitas, K. Scott Weber, Richard A. Robison, O’Neill, Kim L., Larsen, Dallas, Piccolo, Stephen R., Townsend, Michelle H., Stephen R. Piccolo, Kim L. O’Neill, Weber, K. Scott, Tellez Freitas, Claudia M., Michelle H. Townsend, Robison, Richard A., Dallas Larsen   +13 more
core   +1 more source

Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT) [PDF]

, 2005
Includes bibliographical references.The research concerns sub-cloning the gene for HGXPRT from Plasmodium falciparum from a vector with a His-tag facility to one without, expression of the protein in E. coli, and purification.
Mbewe, Boniface
core   +1 more source

Targeting purine metabolism in ovarian cancer

Journal of Ovarian Research, 2022
Purine, an abundant substrate in organisms, is a critical raw material for cell proliferation and an important factor for immune regulation. The purine de novo pathway and salvage pathway are tightly regulated by multiple enzymes, and dysfunction in ...
Jingchun Liu, Shasha Hong, Jiang Yang, Xiaoyi Zhang, Ying Wang, Haoyu Wang, Jiaxin Peng, Li Hong   +7 more
doaj   +1 more source

Red Blood Cells from Individuals with Lesch–Nyhan Syndrome: Multi-Omics Insights into a Novel S162N Mutation Causing Hypoxanthine-Guanine Phosphoribosyltransferase Deficiency

Antioxidants, 2023
Lesch–Nyhan syndrome (LN) is an is an X-linked recessive inborn error of metabolism that arises from a deficiency of purine salvage enzyme hypoxanthine-guanine phosphoribosyltransferase (HPRT).
Julie A. Reisz, Monika Dzieciatkowska, Daniel Stephenson, Fabia Gamboni, D. Holmes Morton, Angelo D’Alessandro   +5 more
doaj   +1 more source

Examination of Hypoxanthine Guanine Phosphoribosyltransferase as a biomarker for colorectal cancer patients

Molecular & Cellular Oncology, 2018
The aim of this study is to investigate these enzymes as possible biomarkers in two colorectal cancer cell lines: HT29, SW480, SW620, and Colo205. With 1,168,929 individuals currently diagnosed with colorectal cancer in the United States, there remains a
Michelle H. Townsend, Abigail M. Felsted, Weston Burrup, Richard A. Robison, Kim L. O’Neill   +4 more
doaj   +1 more source

Data in support of the mutagenic potential of the isoflavone irilone in cultured V79 cells

Data in Brief, 2015
The isoflavone irilone is found in human plasma after ingestion of red clover-based dietary supplements, but information allowing safety assessment is rare.
Anne Scheffler, Annette E. Albrecht, Harald L. Esch, Leane Lehmann   +3 more
doaj   +1 more source