Results 31 to 40 of about 33,169 (270)
Frontiers in Veterinary Science, 2023 Quantitative polymerase chain reaction (qPCR) is an important method to detect gene expression at the molecular level. The selection of appropriate housekeeping genes is the key to accurately calculating the expression level of target genes and ...
Fei Shui +13 more
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BioTechniques, 1999 A lacZ transgene, expressed by the myogenin promoter, was introduced into the mouse hypoxanthine phosphoribosyltransferase (Hprt) locus by gene targeting in embryonic stem cells.
Jay L. Vivian +2 more
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Nucleotide precursors prevent folic acid-resistant neural tube defects in the mouse [PDF]
, 2013 Closure of the neural tube during embryogenesis is a crucial step in development of the central nervous system. Failure of this process results in neural tube defects, including spina bifida and anencephaly, which are among the most common birth defects ...
Copp, AJ +4 more
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dGTP starvation in Escherichia coli provides new insights into the thymineless-death phenomenon.
PLoS Genetics, 2014 Starvation of cells for the DNA building block dTTP is strikingly lethal (thymineless death, TLD), and this effect is observed in all organisms. The phenomenon, discovered some 60 years ago, is widely used to kill cells in anticancer therapies, but many ...
Mark Itsko, Roel M Schaaper
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Targeting purine metabolism in ovarian cancer
Journal of Ovarian Research, 2022 Purine, an abundant substrate in organisms, is a critical raw material for cell proliferation and an important factor for immune regulation. The purine de novo pathway and salvage pathway are tightly regulated by multiple enzymes, and dysfunction in ...
Jingchun Liu +7 more
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DNA-Mediated Transfer of the Adenine Phosphoribosyltransferase Locus into Mammalian Cells [PDF]
, 1979 In this report, we demonstrate the feasibility of transforming mouse cells deficient in adenine phosphoribosyltransferase (aprt; AMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.7) to the aprt+ phenotype by means of DNA-mediated gene transfer ...
Wigler, M. H. +5 more
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Curcumin affects HSP60 folding activity and levels in neuroblastoma cells [PDF]
, 2020 The fundamental challenge in fighting cancer is the development of protective agents able to interfere with the classical pathways of malignant transformation, such as extracellular matrix remodeling, epithelial\u2013mesenchymal transition and ...
Campanella C. +9 more
core +2 more sources
Atrial Natriuretic Peptide, a Regulator of Nuclear Factor-κB Activation in Vivo [PDF]
, 2007 Natriuretic peptides (NPs) comprise a family of vasoactive hormones that play important roles in the regulation of cardiovascular and renal homeostasis.
Keller, Melanie +6 more
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Data in support of the mutagenic potential of the isoflavone irilone in cultured V79 cells
Data in Brief, 2015 The isoflavone irilone is found in human plasma after ingestion of red clover-based dietary supplements, but information allowing safety assessment is rare.
Anne Scheffler +3 more
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Feeding Stimulates Sphingosine-1-Phosphate Mobilization in Mouse Hypothalamus. [PDF]
, 2019 Previous studies have shown that the sphingolipid-derived mediator sphingosine-1-phosphate (S1P) reduces food intake by activating G protein-coupled S1P receptor-1 (S1PR1) in the hypothalamus.
Misto, Alessandra +3 more
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