Results 91 to 100 of about 24,371 (312)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

An early Little Ice Age brackish water invasion along the south coast of the Caspian Sea (sediment of Langarud wetland) and its wider impacts on environment and people [PDF]

, 2015
Caspian Sea level has undergone significant changes through time with major impacts not only on the surrounding coasts, but also offshore. This study reports a brackish water invasion on the southern coast of the Caspian Sea constructed from a multi ...
Aghanabati A, Berberian M, Birks HH, Brückner E, Dolotov Y, Emberger L, Folk RL, Ghamgosar M, Hashemloian BD, Lahijani HAK, Leroy SAG, Molavi-Arabshahi M, Mostowfi H, Rucevska I, Ruddiman WF, Seighali N, Setudeh M, Thompson R, Varushchenko S, Zohary M   +19 more
core   +2 more sources

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Study of sample drilling techniques for Mars sample return missions [PDF]

To demonstrate the feasibility of acquiring various surface samples for a Mars sample return mission the following tasks were performed: (1) design of a Mars rover-mounted drill system capable of acquiring crystalline rock cores; prediction of ...
Harris, P. T., Mitchell, D. C.
core   +1 more source

5. Wochenbericht MSM01/3 [PDF]

, 2006
Maria S. Merian Cruise 1 leg 3 Last report: 10. 05. - 18. 05. 2006 Der 3. Fahrtabschnitt der 1. Reise des neuesten deutschen Forschungsschiffes Maria S. Merian führt in die Schlammvulkanprovinz des Golf von Cadiz.
Pfannkuche, Olaf
core  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

SHALDRIL I and II: Drilling from the Research Vessel Icebreaker Nathaniel B. Palmer [PDF]

Oceanography, 2012
Understanding of Antarctica's climate and ice sheet evolution remains fragmented due to a paucity of outcrops and drill cores that contain deposits from the Neogene (~ 23–2.6 million years ago), when major environmental changes were occurring.
John B. Anderson, Julia S. Wellner
doaj  

Climate variability in SE Europe since 1450 AD based on a varved sediment record from Etoliko Lagoon (Western Greece) [PDF]

, 2017
To achieve deeper understanding of climate variability during the last millennium in SE Europe, we report new sedimentological and paleoecological data from Etoliko Lagoon, Western Greece.
Brauer, Achim, Friedrich, Oliver, Hennrich, Barbara, Koutsodendris, Andreas, Plessen, Birgit, Pross, Jörg, Reed, Jane, Zacharias, Ierotheos   +7 more
core   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source