Results 11 to 20 of about 1,073 (151)
Microorganisms, 2022 Oceans hold a stunning number of unique microorganisms, which remain unstudied by culture-dependent methods due to failures in establishing the right conditions for these organisms to grow.
José Diogo Neves dos Santos +5 more
doaj +4 more sources
Application of iChip to Grow “Uncultivable” Microorganisms and its Impact on Antibiotic Discovery
Journal of Pharmacy & Pharmaceutical Sciences, 2015 Antibiotics have revolutionized modern medicine, allowing significant progress in healthcare and improvement in life expectancy. Development of antibiotic resistance by pathogenic bacteria is a natural phenomenon; however, the rate of antibiotic ...
Rinzhin T. Sherpa +2 more
doaj +6 more sources
A critical role of the Thy28-MYH9 axis in B cell-specific expression of the Pax5 gene in chicken B cells. [PDF]
PLoS ONE, 2015 Accumulating evidence suggests that Pax5 plays essential roles in B cell lineage commitment. However, molecular mechanisms of B cell-specific expression of Pax5 are not fully understood.
Toshitsugu Fujita +2 more
doaj +2 more sources
Direct identification of insulator components by insertional chromatin immunoprecipitation. [PDF]
PLoS ONE, 2011 Comprehensive understanding of mechanisms of epigenetic regulation requires identification of molecules bound to genomic regions of interest in vivo. However, non-biased methods to identify molecules bound to specific genomic loci in vivo are limited ...
Toshitsugu Fujita, Hodaka Fujii
doaj +2 more sources
Molecular Medicine, 2017 More than 100 risk loci for rheumatoid arthritis (RA) have been identified in individuals of European and Asian descent, but the genetic basis for RA in African Americans is less well understood.
Maria I. Danila +10 more
doaj +2 more sources
An Ichip-Domesticated Sponge Bacterium Produces an N-Acyltyrosine Bearing an α-Methyl Substituent
Organic Letters, 2019 The ichip (isolation chip) was employed for the first time in a marine sponge (Xestospongia muta), and a putatively new bacterial species, Alteromonas sp. RKMC-009, was isolated. Strain RKMC-009 produces a novel N-acyltyrosine (1) that is appended with a rare α-methyl substituent within the aminoacyl moiety and also exhibits Gram-positive antibacterial
Logan W. MacIntyre +4 more
core +4 more sources
Locus-specific biochemical epigenetics/chromatin biochemistry by insertional chromatin immnoprecipitation (iChIP) [PDF]
Epigenetics & Chromatin, 2013 Comprehensive understanding of mechanisms of epigenetic regulation requires identification of molecules bound to genomic regions of interest in vivo. However, nonbiased methods to identify molecules bound to specific genomic loci in vivo are limited. To perform biochemical and molecular biological analysis of specific genomic regions, we developed the ...
Fujita, Toshitsugu, Fujii, Hodaka
openaire +2 more sources
Monolithic Chip for High-throughput Blood Cell Depletion to Sort Rare Circulating Tumor Cells
Scientific Reports, 2017 Circulating tumor cells (CTCs) are a treasure trove of information regarding the location, type and stage of cancer and are being pursued as both a diagnostic target and a means of guiding personalized treatment.
Fabio Fachin +19 more
doaj +2 more sources
In situ cultivation of previously uncultivable microorganisms using the ichip
Nature Protocols, 2017 Most microbial species remain uncultivated, and modifying artificial nutrient media brings only an incremental increase in cultivability. We reasoned that an alternative way to cultivate species with unknown requirements is to use naturally occurring combinations of growth factors.
Brittany, Berdy +3 more
openaire +3 more sources
Template Activating Factor-I α Regulates Retroviral Silencing during Reprogramming [PDF]
Cell Reports, 2019 Summary: Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) is accompanied by dramatic changes in epigenetic programs, including silencing of endogenous and exogenous retroviruses. Here, we utilized replication-defective and persistent
Phuong Linh Bui +12 more
doaj +2 more sources