Results 11 to 20 of about 984 (123)

iChip-Inspired Isolation, Bioactivities and Dereplication of Actinomycetota from Portuguese Beach Sediments

Microorganisms, 2022
Oceans hold a stunning number of unique microorganisms, which remain unstudied by culture-dependent methods due to failures in establishing the right conditions for these organisms to grow.
José Diogo Neves dos Santos, Susana Afonso João, Jesús Martín, Francisca Vicente, Fernando Reyes, Olga Maria Lage   +5 more
doaj   +3 more sources

Application of iChip to Grow “Uncultivable” Microorganisms and its Impact on Antibiotic Discovery

Journal of Pharmacy & Pharmaceutical Sciences, 2015
Antibiotics have revolutionized modern medicine, allowing significant progress in healthcare and improvement in life expectancy. Development of antibiotic resistance by pathogenic bacteria is a natural phenomenon; however, the rate of antibiotic ...
Rinzhin T. Sherpa, Caretta J. Reese, Hamidreza Montazeri Aliabadi   +2 more
doaj   +4 more sources

The adaptation chip: repurposing the principles of the ichip for guiding in situ experimental evolution. [PDF]

ISME Commun
Abstract Soil microbial ecosystems are complex and difficult to replicate in laboratory settings. It is often unclear which pressures most strongly shape microbial survival and evolution in situ, and new methods are needed to intersect the manipulative power of the lab with the reality of field environments.
Kaminsky LM, Sadeghi J, Grandinette E, Bell TH.   +3 more
europepmc   +2 more sources

Signal Amplification for Fluorescent Staining of Single Particles in Liquid Biopsies: Circulating Tumour Cells and Extracellular Vesicles. [PDF]

J Extracell Vesicles
ABSTRACT Immunofluorescence (IF) staining represents a convenient and cost‐effective approach to analysing single extracellular vesicles (EVs) and identifying subpopulations with specific roles or biological functions. However, the application of the method is challenged by the weak and unstable signals generated by the low abundant markers carried by ...
Cavallaro S, Veiga SI, Ahmad R, Aldikacti B, Bienstock M, Capen D, Rabe DC, Ho U, Lee D, Ruiz-Torres DA, Wakimoto H, Dietrich J, Nahed BV, Stott SL.   +13 more
europepmc   +2 more sources

Affordable Diffusion Microchamber Array Designs for Isolating Microbes in Classrooms and Laboratories

Journal of Microbiology & Biology Education, 2021
Diffusion microchamber array (DMA) devices have provided contemporary microbiologists with a successful alternative to the century-old plating method using petri dishes to isolate and cultivate environmental bacteria. The “ichip” DMA device, developed by
Michael J. Braus, Robert Swader, George Petry, Thea Whitman   +3 more
doaj   +1 more source

A critical role of the Thy28-MYH9 axis in B cell-specific expression of the Pax5 gene in chicken B cells. [PDF]

PLoS ONE, 2015
Accumulating evidence suggests that Pax5 plays essential roles in B cell lineage commitment. However, molecular mechanisms of B cell-specific expression of Pax5 are not fully understood.
Toshitsugu Fujita, Fusako Kitaura, Hodaka Fujii   +2 more
doaj   +1 more source

Direct identification of insulator components by insertional chromatin immunoprecipitation. [PDF]

PLoS ONE, 2011
Comprehensive understanding of mechanisms of epigenetic regulation requires identification of molecules bound to genomic regions of interest in vivo. However, non-biased methods to identify molecules bound to specific genomic loci in vivo are limited ...
Toshitsugu Fujita, Hodaka Fujii
doaj   +1 more source

Use of Ichip for High-Throughput In Situ Cultivation of “Uncultivable” Microbial Species [PDF]

Applied and Environmental Microbiology, 2010
ABSTRACT One of the oldest unresolved microbiological phenomena is why only a small fraction of the diverse microbiological population grows on artificial media. The “uncultivable” microbial majority arguably represents our planet's largest unexplored pool of biological and chemical novelty. Previously we showed that species from
D, Nichols, N, Cahoon, E M, Trakhtenberg, L, Pham, A, Mehta, A, Belanger, T, Kanigan, K, Lewis, S S, Epstein   +8 more
openaire   +2 more sources

Novel and Conventional Isolation Techniques to Obtain Planctomycetes from Marine Environments

Microorganisms, 2021
Bacteria from the distinctive Planctomycetes phylum are well spread around the globe; they are capable of colonizing many habitats, including marine, freshwater, terrestrial, and even extreme habitats such as hydrothermal vents and hot springs.
Inês Vitorino, José Diogo Neves Santos, Ofélia Godinho, Francisca Vicente, Vítor Vasconcelos, Olga Maria Lage   +5 more
doaj   +1 more source

Chromatin unwinding state for in situ identification and lineage tracing of circulating tumor cells. [PDF]

Imeta
The detection of circulating tumor cells (CTCs) through liquid biopsy offers a non‐invasive approach for accurately monitoring cancer dissemination and evaluating therapeutic efficiency. However, their rarity and heterogeneity limit conventional tumor antigen labelling‐based methods in identifying and tracing CTCs.
Ye B, Wang Z, Bai Y, Zhang X, Zhang R, Lian J, Liu X, Zhang Y, Xu Z, Yang L, Jin H, Chen F, Xie Z, Zhou P, Tan J, Zeng S, Du C, Min Y, Ni X, Duan J, Li Z, Yang H, Cai Y, Wu H, Liu CC, Cai J, Lu Y, Zhang J, Wu H, Zheng H, Liu L, Hong X, Yu H.   +32 more
europepmc   +2 more sources