Results 241 to 250 of about 156,271 (269)
Reproducibility of methylated CpG typing with the Illumina MiSeq [PDF]
Forensic Science International: Genetics Supplement Series, 2017 DNA methylation patterns may be used for identification of body fluids and for age estimation of human individuals. We evaluated some of the challenges and pitfalls of studying methylated CpG sites.
Claus Børsting
exaly +4 more sources
Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms [PDF]
ISME Journal, 2012 DNA sequencing continues to decrease in cost with the Illumina HiSeq2000 generating up to 600 Gb of paired-end 100 base reads in a ten-day run. Here we present a protocol for community amplicon sequencing on the HiSeq2000 and MiSeq Illumina platforms ...
Jack A Gilbert +2 more
exaly +7 more sources
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Next-Generation Bisulfite Sequencing Of Cacna1c With Illumina Miseq
European Neuropsychopharmacology, 2017Background The complex etiology of psychiatric illness involves both genetic and environmental factors. The latter may act via epigenetic processes such as DNA methylation. Studies suggest that epigenetic regulation of gene expression may mediate the effects of early life experiences on adult behavior and susceptibility to psychiatric illness ...
Helene Dukal +8 more
openaire +1 more source
Microbiota Analysis Using an Illumina MiSeq Platform to Sequence 16S rRNA Genes
Current Protocols in Mouse Biology, 2017AbstractThe microbiota have been shown to play an important role in diverse biological processes including immunity, metabolism, and digestion. Assessing the exact composition of the microbiota has proven challenging due to the often unknown growth specificities of its members, and culture‐based approaches typically fail to capture the complete ...
Rapin, Alexis +3 more
openaire +4 more sources
Systematic assessment of the performance of Illumina's MiSeq FGx™ forensic genomics system
ELECTROPHORESIS, 2017This study assesses the performance of Illumina's MiSeq FGx System for forensic genomics by systematically analyzing single source samples, evaluating concordance, sensitivity and repeatability, as well as describing the quality of the reported outcomes. DNA from 16 individuals (9 males/7 females) in nine separate runs showed consistent STR profiles at
Nouran, Almalki +5 more
openaire +2 more sources
Genotyping polymorphic microhaplotype markers through the Illumina® MiSeq platform for forensics
Forensic Science International: Genetics, 2019Microhaplotype markers are emerging forensic genetic markers that have received broad attention in forensics and may supplement existing genetic marker panels. Short tandem repeat polymorphisms (STRPs) and single nucleotide polymorphisms (SNPs) are the general genetic markers at present.
Jing Zhu +15 more
openaire +2 more sources
A Comparative study of HLA Typing using an illumina miseq NGS System
Human Immunology, 2015Aim Although the Luminex system for HLA typing provides a convenient, easy to perform laboratory workflow, limitations restricting the ability to routinely obtain both breadth (number of loci) and depth (allele resolution) of data are inherent in the system.
Hiroto Kojima +12 more
openaire +1 more source
Illumina MiSeq – primjena na Hrvatskom veterinarskom institutu
2023Illumina MiSeq – primjena na Hrvatskom veterinarskom ...
openaire
Marine bacterial community analysis on 316L stainless steel coupons by Illumina MiSeq sequencing
Biotechnology Letters, 2020In order to evaluate the corrosive action of microorganisms on 316L metal exposed directly to a marine environment, a system was designed to immerse coupons in seawater. After periods of 30, 60 and 90 days, the coupons were recovered, the corrosion rates evaluated and the biofilm samples on their surface were analyzed by 16S rRNA gene sequencing.
Artur Capão +4 more
openaire +2 more sources
2016
This protocol details the analysis of Illumina MiSeq amplicon libraries derived from marine benthic macroinvertebrate samples and based on two barcodes of the mitochondrial cytochrome oxidase 1 (CO1) gene: a "short region," covered by overlapping forward and reverse reads and a "long region" for which forward and reverse reads do not overlap.
Eva, Aylagas +1 more
openaire +2 more sources
This protocol details the analysis of Illumina MiSeq amplicon libraries derived from marine benthic macroinvertebrate samples and based on two barcodes of the mitochondrial cytochrome oxidase 1 (CO1) gene: a "short region," covered by overlapping forward and reverse reads and a "long region" for which forward and reverse reads do not overlap.
Eva, Aylagas +1 more
openaire +2 more sources