Results 101 to 110 of about 34,558,744 (307)

Multiple tissue antigen analysis by sequential immunofluorescence staining and multi-dimensional image analysis

, 2001
This paper presents a novel method for sequential immunofluorescence staining, which, in combination with 3D image registration and segmentation, can be used to increase the number of antigens that can be observed simultaneously in single cells in tissue
Lindblad, Joakim,, Wählby, Carolina,, Zetterberg, Anders, Bengtsson, Ewert,, Nyberg, Karl, Erlandsson, Fredrik   +5 more
core   +5 more sources

Deep learning methods for noisy sperm image classification from convolutional neural network to visual transformer: a comprehensive comparative study

Intelligent Medicine
Background With the gradual increase of infertility in the world, among which male sperm problems are the main factor for infertility, more and more couples are using computer-assisted sperm analysis (CASA) to assist in the analysis and treatment of ...
Ao Chen, Chen Li, Md Mamunur Rahaman, Yudong Yao, Haoyuan Chen, Hechen Yang, Peng Zhao, Weiming Hu, Wanli Liu, Shuojia Zou, Ning Xu, Marcin Grzegorzek   +11 more
doaj   +1 more source

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas F. Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

Image Analysis and Reconstruction using a Wavelet Transform Constructed from a Reducible Representation of the Euclidean Motion Group

, 2007
Inspired by the early visual system of many mammalians we consider the construction of-and reconstruction from- an orientation score Uf:R2×S1→C as a local orientation representation of an image, f:R2→R .
Felsberg, M, Haar Romenij, ter, B.M., Granlund, Gösta,, Duits, Remco, Duits, R., Granlund, G., Granlund, G, Duits, Remco,, Felsberg, M., Granlund, Gösta, Duits, R Remco, Haar Romeny, BM Bart ter, ter Haar Romeny, Bart M., ter Haar Romeny, Bart M.,, Felsberg, Michael, Felsberg, Michael,   +15 more
core   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Linear image reconstruction by Sobolev norms on the bounded domain [PDF]

, 2007
The reconstruction problem is usually formulated as a variational problem in which one searches for that image that minimizes a so called prior (image model) while insisting on certain image features to be preserved.
Haar Romenij, ter, B.M., Paragios, N., Murli, A., Duits, R., Sgallari, F., Janssen, B.J.   +5 more
core   +1 more source

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

FEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani, Konstantinos Spanopoulos, Zoi Piperigkou, Nikos K. Karamanos   +3 more
wiley   +1 more source

The truth is hard to make: validation of medical image registration

, 2017
An unsolved problem in medical image analysis is validation of methods. In this paper we will focus on image registration and in particular on nonlinear image registration, which is one of the hardest analysis problems to validate.
Pluim, J.P.W., Eppenhof, K.A.J.; id_orcid, Eppenhof, KAJ Koen, Muenzing, SEA, Murphy, K, Muenzing, S.E.A., Eppenhof, K.A.J., Murphy, K., Pluim, JPW Josien   +8 more
core   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source