Results 51 to 60 of about 214,502 (252)

DNA Cytometry Image Analysis Software

Acta Biologica Sibirica
The article presents the development of BioDecod, a tool for the automated detection and quantification of cell nuclei in images. BioDecod enables accurate nuclei identification and counting, measurement of their fluorescence intensity, and generation of
Maxim G. Kutsev, Roman N. Panarin, Michael V. Skaptsov, Kseniya K. Ryabova, Olga V. Uvarova, Anastasia M. Koltunova   +5 more
doaj   +1 more source

Overcoming primary and acquired resistance to anti-PD-L1 therapy by induction and activation of tumor-residing cDC1s

Nature Communications, 2020
Strategies to increase T cell infiltration within the tumor microenvironment could improve response to immune checkpoint blockade. Here the authors show that a combinatorial regimen based on Flt3L, radiotherapy, and TLR3/CD40 stimulation promotes ...
Takaaki Oba, Mark D. Long, Tibor Keler, Henry C. Marsh, Hans Minderman, Scott I. Abrams, Song Liu, Fumito Ito   +7 more
doaj   +1 more source

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

Immune signatures associated with improved progression-free and overall survival for myeloma patients treated with AHSCT

Blood Advances, 2017
: Multiple therapeutic options exist for multiple myeloma (MM), including autologous hematopoietic stem cell transplantation (AHSCT). Measurement of minimal residual disease (MRD) and immune reconstitution is rapidly becoming an integral part of the care
Christine M. Ho, Philip L. McCarthy, Paul K. Wallace, Yali Zhang, Ahmad Fora, Patrick Mellors, Joseph D. Tario, Benjamin L.S. McCarthy, George L. Chen, Sarah A. Holstein, Sophia R. Balderman, Xuefang Cao, Bruno Paiva, Theresa Hahn   +13 more
doaj   +1 more source

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Diagnostic DNA-Flow- vs. -Image-Cytometry in Effusion Cytology

Analytical Cellular Pathology, 2002
Aims: To determine the sensitivity and specificity of flow‐ and image‐cytometry for the detection of DNA‐aneuploidy as a marker for malignant cells in effusions.
Helma Motherby, Natalia Pomjanski, Mary Kube, Alexandra Boros, Thomas Heiden, Bernhard Tribukait, Alfred Böcking   +6 more
doaj   +1 more source

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source

Tissue Cytometry Assay with Nuclear Segmentation for Quantifying NETotic Cells in Neutrophils Stimulated by Spermatozoa in Veterinary Species

Animals
Upon activation, neutrophils perform three distinct functions: phagocytosis, degranulation of antimicrobial substances into the extracellular medium, and release of neutrophil extracellular traps.
Rodrigo Rivera-Concha, Marion León, Nikol Ponce-Rojas, Aurora Prado-Sanhueza, Pamela Uribe, Anja Taubert, Carlos Hermosilla, Raúl Sánchez, Fabiola Zambrano   +8 more
doaj   +1 more source

Photothermal image cytometry of human neutrophils [PDF]

Cytometry, 1996
Photothermal imaging, when applied to the study of living cells, provides morphofunctional information about cell populations. In technical terms, the method is complementary to optical microscopy. The photothermal method was used for cell imaging and quantitative studies. We have developed a dual-laser technique and have constructed an automated image
D, Lapotko, G, Kuchinsky, M, Potapnev, D, Pechkovsky   +3 more
openaire   +2 more sources

ZW4864‐mediated inhibition of the β‐catenin/BCL9/BCL9L complex reveals therapeutic potential in bladder cancer

Molecular Oncology, EarlyView.
BCL9 and BCL9L drive bladder cancer progression by enhancing β‐catenin signaling, promoting proliferation, migration, invasion, and organoid growth. Genetic depletion of BCL9(L) suppresses malignant phenotypes, while pharmacological disruption of the β‐catenin/BCL9(L) complex with ZW4864 inhibits canonical Wnt signaling and tumor‐associated cellular ...
Roland Kotolloshi, Mandy Berndt‐Paetz, Eileen Lerner, Gregoire Najjar, Anca Azoitei, Krishna Pal Singh, Shailendra Kumar Gupta, Olaf Wolkenhauer, Cagatay Günes, Otmar Huber, Marc‐Oliver Grimm, Daniel Steinbach   +11 more
wiley   +1 more source