Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose +9 more
wiley +1 more source
Effect of biochar on heavy metal immobilization in contaminated soil [PDF]
Soil contamination by potentially toxic metals is a serious environmental problem faced worldwide, hence sustainable and effective amendment methods are required.
Noumbissi Ngambia, Audrey Lucrece
core
Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana +5 more
wiley +1 more source
Imobilização de celulase em nanopartículas poliméricas de poli(metacrilato de metila) via polimerização em miniemulsão [PDF]
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia Química, Florianópolis, 2015.Celulases são enzimas altamente eficientes na conversão de celulose em glicose. Seu emprego na forma
Simon, Patricia
core
Effect of Immobilization on the Activity of Rat Hepatic-Microsomal Cytochrome P450 Enzymes [PDF]
Cytochrome P450 enzyme preparations were obtained from phenobarbital-treated rats.'On immobilization in Romicon PM 10 polysulfone anisotropic hollow fibers, 20-25% of the original activity remained.
Bunch, A.W. +2 more
core +1 more source
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Imobilização de lipase B de Candida antarctica em espuma de poliuretano e aplicação na síntese do éster geranil propionato [PDF]
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia de Alimentos, 2014.Lipase B de Candida antarctica (CalB) apresenta um papel importante na indústria química e de alimentos.
Nicoletti, Gabrieli
core
EDC-mediated oligonucleotide immobilization on a long period grating optical biosensor [PDF]
We present the development and simplification of label-free fiber optic biosensors based on immobilization of oligonucleotides on dual-peak long period gratings (dLPGs).
Chen, Xianfeng +5 more
core +1 more source
Protein immobilization technology for flow biocatalysis [PDF]
Enzymatic immobilization has been at the forefront of applied biocatalysis as it enables convenient isolation and reuse of the catalyst if the target reaction is conducted in batch, and it has opened up significant opportunities to conduct biocatalysis ...
Romero-Fernández, María +1 more
core +1 more source

