Results 71 to 80 of about 247,213 (311)

Enzyme Immobilization on Cellulose Carriers

, 2021
Imobilizacija enzima je vezanje enzima na nosač, a jedni od najidealnijih nosača za imobilizaciju enzima su celuloza i njezini derivati. Neke od metoda kojima se provodi imobilizacija enzima na celulozne nosače su kovalentna imobilizacija, metoda ...
Didović, Iva
core  

Effect of acute immobilization on the hematological, red-ox and rheological parameters of erythrocytes in rats [PDF]

Al Ameen Journal of Medical Sciences, 2013
Objectives: Immobilization effects on antioxidant enzymes activities, red-ox variables and rheological parameters of erythrocytes were studied. Background: It is not clear to which extent immobilization-induced oxidative stress may affect erythrocyte ...
Artemy V. Novozhilov, Tat’yana V. Tavrovskaya, Michael I. Kalinski, Lev N. Katyukhin, Vadim A. Ivanov, Vladimir I. Morozov   +5 more
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Enzyme Immobilization by Polyethylenimine

, 2021
Polietilenimin (PEI) sintetski je kationski polimer čija funkcionalnost i reaktivnost potječu od činjenice da se radi o polimeru koji sadrži primarne, sekundarne i tercijarne skupine amina.
Lendić, Barbara
core  

Effects of electroacupuncture on stress and anxiety-related responses in rats

Anais da Academia Brasileira de Ciências
The aim of this work was to investigate if eletroacupuncture at PC6 would modulate the stress-induced anxiety-like behavior and the level of activation of several brain areas.
RICARDO M. BASSETTO, TATIANA WSCIEKLICA, KATHLEIN C.P. POUZA, DANIELA ORTOLANI, MILENA B. VIANA, ISABEL C. CESPEDES, REGINA C. SPADARI   +6 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

A new approach to evaluate immobilization of β-galactosidase on Eupergit® C: structural, kinetic, and thermal characterization

Química Nova, 2014
This study aimed to evaluate β-galactosidase immobilization. For this purpose, the ionic strength of the buffer, reaction time, amount of the immobilization support, and pH were evaluated by a central composite design. Assay 8, which consisted of 1.5 mol
Anna R. C. Braga, Marceli F. Silva, José V. Oliveira, Helen Treichel, Susana J. Kalil   +4 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Immobilization of Glycoside Hydrolase Families GH1, GH13, and GH70: State of the Art and Perspectives

Molecules, 2016
Glycoside hydrolases (GH) are enzymes capable to hydrolyze the glycosidic bond between two carbohydrates or even between a carbohydrate and a non-carbohydrate moiety.
Natália G. Graebin, Jéssie da N. Schöffer, Diandra de Andrades, Plinho F. Hertz, Marco A. Z. Ayub, Rafael C. Rodrigues   +5 more
doaj   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source