Designing multifunctional biocatalytic cascade system by multi-enzyme co-immobilization on biopolymers and nanostructured materials [PDF]
, 2023 The research leading to these results has received funding from the Norwegian Financial Mechanism 2014-2021 under Project number 2020/37/K/ST8/03805.
Jesionowski, Teofil +7 more
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Molecular Oncology, EarlyView.Breast cancer remains a major cause of cancer death in women, frequently developing endocrine therapy resistance. This study demonstrates that upregulated p21‐activated kinase 1 (PAK1) activity drives resistance to tamoxifen and long‐term estrogen deprivation in ER+ breast cancer models.
Luisa Schwarzmüller +10 more
wiley +1 more source
Spatial biology in cancer epigenetics
Molecular Oncology, EarlyView.Spatial epigenomics combines molecular profiling with tissue architecture to reveal how gene regulation is organized within intact tissues. In cancer, these technologies uncover the mechanisms driving tumor heterogeneity and microenvironmental interactions, opening new opportunities for biomarker discovery and precision medicine.
Eva Crespo‐García, Manel Esteller
wiley +1 more source
Chemical immobilization of free-ranging Fallow deer in Northers Italy: a retrospective observational study [PDF]
, 2023 Induction time is the most important factor when considering wildlife immobilization, and it can widely depend on breed, sex, age class, anaesthesiologic protocol and needle length.
Riccardo Ercolini +2 more
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Improved enzymatic activity by oriented immobilization on graphene oxide with tunable surface heterogeneity [PDF]
, 2021 The precision design of surface properties of supports and immobilization methodologies is essential for improving the biocatalytic activity of immobilized enzymes. In this study, we proposed a surface heterogeneity regulation strategy of graphene oxide (
Ge, Lei +11 more
core +1 more source
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Hydrophobic adsorption and covalent immobilization of Candida antarctica lipase B on mixed-function-grafted silica gel supports for continuous-flow biotransformations [PDF]
, 2013 Adsorption onto solid supports has proven to be an easy and effective way to improve the mechanical and catalytic properties of lipases. Covalent binding of lipases onto the support surface enhances the active lifetime of the immobilized biocatalysts ...
Poppe, László +8 more
core +1 more source
Journal of Materials Research and Technology, 2019 In recent years, metal–organic frameworks (MOFs) have received accelerating research attention as a versatile carrier and promising bio-immobilization support materials for enzyme immobilization. This is particularly due to their extraordinary structural
Muhammad Bilal +3 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Desenvolvimento de matriz de imobilização de lipase utilizando gelatina de diferentes blooms adicionada de plastificantes hidrofílicos [PDF]
, 2012 Tese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia QuímicaO emprego de enzimas imobilizadas vem aumentando devido às vantagens que as mesmas oferecem como, por exemplo, a possibilidade ...
Kempka, Aniela Pinto
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