Results 81 to 90 of about 635,875 (407)

Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR) Imaging

, 2013
An immobilization protocol was developed to attach receptors on smooth silver thin films. Dense and packed 11-mercaptoundecanoic acid (11-MUA) was used to avoid uncontrolled sulfidization and harmful oxidation of silver nanolayers.
Abdulhalim, Ibrahim, Robert Marks, Lev Tsapovsky, Tsapovsky, Lev, Alina Karabchevsky, Ibrahim Abdulhalim, Robert S. Marks, Karabchevsky, Alina, Marks, Robert   +8 more
core   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Industrial applications of fungal lipases: a review

Frontiers in Microbiology, 2023
Fungal lipases (triacylglycerol acyl hydrolases EC 3.1.1.3) are significant industrial enzymes and have several applications in a number of industries and fields. Fungal lipases are found in several species of fungi and yeast.
Ashish Kumar, Vinita Verma, Vimal Kumar Dubey, Alok Srivastava, Sanjay Kumar Garg, Vijay Pal Singh, Pankaj Kumar Arora   +6 more
doaj   +1 more source

関節の不動が膝関節屈曲拘縮モデルラットの関節包における筋線維芽細胞とコラーゲンの変化におよぼす影響

, 2017
The purpose of this study was to examine the time-dependent changes in the development of joint capsule fibrosis and in the number of myofibroblasts in the joint capsule after immobilization, using a rat knee contracture model.
佐々部, 陵
core   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Multifunctional metal–organic frameworks-based biocatalytic platforms: recent developments and future prospects

Journal of Materials Research and Technology, 2019
In recent years, metal–organic frameworks (MOFs) have received accelerating research attention as a versatile carrier and promising bio-immobilization support materials for enzyme immobilization. This is particularly due to their extraordinary structural
Muhammad Bilal, Muhammad Adeel, Tahir Rasheed, Hafiz M.N. Iqbal   +3 more
doaj   +1 more source

Comparison of toe grip strength and muscle activities during maximal toe grip strength exertion according to the presence/absence of an ankle immobilization belt

, 2015
[Purpose] The aim of this study was to compare toe grip strength and muscle activity during toe grip strength exertion according to the presence/absence of an ankle immobilization belt and to examine the relationship between the differences in muscle ...
Satou, Yousuke, Murata, Jun, Nakae, Hideyuki, Soma, Masayuki, Kai, Yoshihiro, Murata, Shin, Miyazaki, Junya   +6 more
core   +1 more source

Hydrophobic adsorption and covalent immobilization of Candida antarctica lipase B on mixed-function-grafted silica gel supports for continuous-flow biotransformations [PDF]

, 2013
Adsorption onto solid supports has proven to be an easy and effective way to improve the mechanical and catalytic properties of lipases. Covalent binding of lipases onto the support surface enhances the active lifetime of the immobilized biocatalysts ...
Poppe, László, Márkus, Mária, Abaháziová, Emese, Weiser, Diána, Kovács, Péter, Koczkac, Béla, Boros, Zoltán, Tomin, Anna, Magyar, Ágnes   +8 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source