Results 61 to 70 of about 378,681 (314)

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Effects of detergents on activity, thermostability and aggregation of immobilized lipases [PDF]

, 2014
Predmetom tejto diplomovej práce bolo štúdium vplyvu detergentov na aktivitu, termostabilitu a agregáciu voľnej a imobilizovanej formy komerčného preparátu lipázy izolovanej z mikroskopickej huby Rhizopus arrhizus. Teoretická časť obsahuje ucelenú rešerš
Bančáková, Anna
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Effect of acute immobilization on the hematological, red-ox and rheological parameters of erythrocytes in rats [PDF]

Al Ameen Journal of Medical Sciences, 2013
Objectives: Immobilization effects on antioxidant enzymes activities, red-ox variables and rheological parameters of erythrocytes were studied. Background: It is not clear to which extent immobilization-induced oxidative stress may affect erythrocyte ...
Artemy V. Novozhilov, Tat’yana V. Tavrovskaya, Michael I. Kalinski, Lev N. Katyukhin, Vadim A. Ivanov, Vladimir I. Morozov   +5 more
doaj  

Multiplexed in-gel microfluidic immunoassays: characterizing protein target loss during reprobing of benzophenone-modified hydrogels. [PDF]

, 2019
From whole tissues to single-cell lysate, heterogeneous immunoassays are widely utilized for analysis of protein targets in complex biospecimens. Recently, benzophenone-functionalized hydrogel scaffolds have been used to immobilize target protein for ...
Gopal, Anjali, Herr, Amy E
core   +1 more source

Mediator-free interaction of glucose oxidase, as model enzyme for immobilization, with Al-doped and undoped ZnO thin films laser-deposited on polycarbonate supports [PDF]

, 2017
Al doped and undoped ZnO thin films were deposited by pulsed-laser deposition on polycarbonate sheets. The films were characterized by optical transmission, Hall effect measurement, XRD and SEM.
Inguva, Saikumar, Krishnamurthy, Satheesh, Kumar, Fidal, Marsili, Enrico, Mosnier, Jean-Paul, Sainathan, Chandra   +5 more
core   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Effects of electroacupuncture on stress and anxiety-related responses in rats

Anais da Academia Brasileira de Ciências
The aim of this work was to investigate if eletroacupuncture at PC6 would modulate the stress-induced anxiety-like behavior and the level of activation of several brain areas.
RICARDO M. BASSETTO, TATIANA WSCIEKLICA, KATHLEIN C.P. POUZA, DANIELA ORTOLANI, MILENA B. VIANA, ISABEL C. CESPEDES, REGINA C. SPADARI   +6 more
doaj   +1 more source

Chromoionophores in optical ion sensors [PDF]

, 1989
The feasibility of surface plasmon resonance (SPR) for ion sensing has been investigated. Emphasis is laid on a simulation-based optimization of the SP carrying structure, as well as the applicability of a specific chemo—optical interface we have ...
Gent, J. van, Gerritsma, G.J., Kreuwel, H.J.M., Lambeck, P.V., Popma, Th.J.A., Reinhoudt, D.N., Sudhölter, E.J.R.   +6 more
core   +2 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source