Results 41 to 50 of about 464,228 (291)

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Recent advances in enzyme immobilization technology

Xi'an Gongcheng Daxue xuebao
As an efficient and safe biocatalyst, enzymes have been widely used in many fields such as food, medicine and environmental governance, but it is difficult to realize further industrial application due to the poor environmental stability of free enzymes.
PAN Hong, LU Tianwen, WANG Xiaojun, HONG Yinan   +3 more
doaj   +1 more source

Parameters Affecting the Performance of Immobilized Enzyme

Journal of Chemistry, 2013
Enzyme immobilization has been investigated to improve lipase properties over the past few decades. Different methods and various carriers have been employed to immobilize enzyme.
Dong-Hao Zhang, Li-Xia Yuwen, Li-Juan Peng   +2 more
doaj   +1 more source

Effects of 2 weeks lower limb immobilization and two separate rehabilitation regimens on gastrocnemius muscle protein turnover signaling and normalization genes

BMC Research Notes, 2012
Background Limb immobilization causes a rapid loss of muscle mass and strength that requires appropriate rehabilitation to ensure restoration of normal function.
Nedergaard Anders, Jespersen Jakob G, Pingel Jessica, Christensen Britt, Sroczynski Nicholas, Langberg Henning, Kjaer Michael, Schjerling Peter   +7 more
doaj   +1 more source

Forest Soil Carbon and Nitrogen Cycles under Biomass Harvest: Stability, Transient Response, and Feedback [PDF]

, 2016
Biomass harvest generates an imbalance in forest carbon (C) and nitrogen (N) cycles and the nonlinear biogeochemical responses may have long-term consequences for soil fertility and sustainable management.
Parolari, Anthony J., Porporato, Amilcare   +1 more
core   +2 more sources

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases

Molecules, 2014
The main objective of the immobilization of enzymes is to enhance the economics of biocatalytic processes. Immobilization allows one to re-use the enzyme for an extended period of time and enables easier separation of the catalyst from the product ...
Urszula Guzik, Katarzyna Hupert-Kocurek, Danuta Wojcieszyńska   +2 more
doaj   +1 more source

Integrate range shifter in immobilization for proton therapy: 3D printed materials characterization [PDF]

, 2016
3D printing is investigated for application in patient immobilization during proton therapy (PT). It potentially enables a merge of immobilization, range shifting and other functionality into one patient-specific structure.
D'Hollander, Antoine, Denis, Jean-Marc, Depuydt, Tom, Haustermans, Karin, Kersemans, Mathias, Lammens, Nicolas, Michiels, Steven, Nuyts, Sandra, Sterpin, Edmond, Zhang, Guozhi   +9 more
core   +1 more source

Cellulase immobilization on superparamagnetic nanoparticles for reuse in cellulosic biomass conversion [PDF]

, 2016
Current cellulosic biomass hydrolysis is based on the one-time use of cellulases. Cellulases immobilized on magnetic nanocarriers offer the advantages of magnetic separation and repeated use for continuous hydrolysis. Most immobilization methods focus on
Fernando Segato, Mark Wilkins, null null, Qing Song, Rolf Prade, Yu Mao   +5 more
core   +3 more sources