Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients [PDF]
Membrane and nuclear proteins of poor solubility have been separated by high resolution two-dimensional (2-D) gel electrophoresis. Isoelectric focusing with immobilized pH gradients leads to severe quantitative losses of proteins in the resulting 2-D map, although the resolution is usually high.
arxiv +1 more source
Immobilization of lactase to Perloza cellulose resins : a thesis was presented in partial fulfilment of the requirements for the degree of Master of Science in Chemistry at Massey University [PDF]
A bead cellulose matrix, Perloza, was chemically modified by two attachment chemistries to prepare inexpensive resins for immobilization of lactase. A commercial product, the base-activated matrix Eupergit C was studied for comparison.
Yu, Min
core
Enzymatic AND-Gate Based on Electrode-Immobilized Glucose-6-Phosphate Dehydrogenase: Towards Digital Biosensors and Biochemical Logic Systems with Low Noise [PDF]
Electrode-immobilized glucose-6-phosphate dehydrogenase is used to catalyze an enzymatic reaction which carries out the AND logic gate. This logic function is considered here in the context of biocatalytic processes utilized for the biocomputing applications for "digital" (threshold) sensing/actuation.
arxiv +1 more source
Ion transport through confined ion channels in the presence of immobile charges [PDF]
We study charge transport in an ionic solution in a confined nanoscale geometry in the presence of an externally applied electric field and immobile background charges. For a range of parameters, the ion current shows non-monotonic behavior as a function of the external ion concentration.
arxiv +1 more source
Towards 3D printed multifunctional immobilization for proton therapy: initial materials characterization [PDF]
Purpose: 3D printing technology is investigated for the purpose of patient immobilization during proton therapy. It potentially enables a merge of patient immobilization, bolus range shifting, and other functions into one single patient-speci c structure.
Ahn+44 more
core +2 more sources
Permeability of immobile rings of membrane inclusions to in-plane flow [PDF]
We study the flow of membranal fluid through a ring of immobile particles mimicking, for example, a fence around a membrane corral. We obtain a simple closed-form expression for the permeability coefficient of the ring as a function of the particles' line fraction.
arxiv +1 more source
Forest Soil Carbon and Nitrogen Cycles under Biomass Harvest: Stability, Transient Response, and Feedback [PDF]
Biomass harvest generates an imbalance in forest carbon (C) and nitrogen (N) cycles and the nonlinear biogeochemical responses may have long-term consequences for soil fertility and sustainable management.
Parolari, Anthony J.+1 more
core +2 more sources
Evaluation of daily residual pancreatic tumor motion for deep‐inspiration breath‐hold radiotherapy
Abstract Purpose Breath‐hold techniques are widely used in radiation therapy to minimize respiratory‐induced tumor or organ‐at‐risk motion. However, residual motion persists, necessitating a reliable daily evaluation method. Methods At our institution, fiducial markers serve as surrogates for target localization in pancreatic cancer treatment.
Weihua Mao+4 more
wiley +1 more source
Investigations on vinylene carbonate. V. Immobilization of alkaline phosphatase onto LDPE films cografted with vinylene carbonate and N-vinyl-N-methylacetamide [PDF]
Low-density polyethylene (LDPE) films cografted with vinylene carbonate (VCA) and N-vinyl-N-methylacetamide (VIMA) were studied as a matrix for the immobilization of the enzyme alkaline phosphatase (ALP) either by direct fixation or by inserting spacers.
Bantjes, Adriaan+2 more
core +4 more sources
Application in the Ethanol Fermentation of Immobilized Yeast Cells in Matrix of Alginate/Magnetic Nanoparticles, on Chitosan-Magnetite Microparticles and Cellulose-coated Magnetic Nanoparticles [PDF]
Saccharomyces cerevisiae cells were entrapped in matrix of alginate and magnetic nanoparticles and covalently immobilized on magnetite-containing chitosan and cellulose-coated magnetic nanoparticles. Cellulose-coated magnetic nanoparticles with covalently immobilized thermostable {\alpha}-amylase and chitosan particles with immobilized glucoamylase ...
arxiv