Results 21 to 30 of about 352,107 (336)

3D projection electrophoresis for single-cell immunoblotting

open access: yesNature Communications, 2019
Immunoassays and mass spectrometry are powerful single-cell protein analysis tools; however, interfacing and throughput bottlenecks remain. Here, we introduce three-dimensional single-cell immunoblots to detect both cytosolic and nuclear proteins. The 3D
S. Grist   +2 more
semanticscholar   +1 more source

Study of specific IgE and IgG4 antibodies to mite antigen in asthmatic children during immunotherapy

open access: yesAllergology International, 1998
Using immunoblotting and densitometer techniques, we followed the levels of IgE and IgG4 antibodies to Dermatophagoides farinae antigen in the sera of 12 asthmatic children during immunotherapy, repeated injections of house dust extract, for 2 years. The
Hiroyuki Matsuoka   +7 more
doaj   +1 more source

Recombinant Protein p30 for Serological Diagnosis of African Swine Fever by Immunoblotting Assay

open access: yesTransboundary and Emerging Diseases, 2017
This article is devoted to the development and evaluation of the immunoblotting test system for serological diagnosis of African swine fever (ASF), based on the highly purified recombinant p30 of ASF virus (ASFV) strain Stavropol 01/08 (Stavropol 2008 ...
A. Kazakova   +7 more
semanticscholar   +1 more source

Immunoblotting for the serodiagnosis of Helicobacter pylori  infection in Brazilian patients with and without gastric carcinoma

open access: yesMemorias do Instituto Oswaldo Cruz, 2004
We evaluated the performance of a commercial immunoblotting in the serodiagnosis of Helicobacter pylori infection in Brazilian patients. The presence of anti-H.
Andreia MC Rocha   +5 more
doaj   +1 more source

Heterologous expression of two acetylcholinesterases of colorado potato beetle Leptinotarsa decemlineata in bacteria Escherichia coli and production of form-specific antibodies

open access: yesВестник защиты растений, 2020
The Colorado potato beetle Leptinotarsa decemlineata is a widespread pest of plants of the Solanaceae family. Huge economic loss caused by L. decemlineata around the world is multiplied by its ability to develop resistance to all major insecticide ...
Вячеслав Васильевич Долгих   +6 more
doaj   +1 more source

Diagnostic markers of yersiniosis in patients with inflammatory bowel diseases

open access: yesЭпидемиология и вакцинопрофилактика, 2023
Relevance. This study is related to the study of the etiology, pathogenesis and clinic of inflammatory bowel diseases (IBD). In a number of patients with IBD who are refractory to anti-inflammatory/immunosuppressive therapy, there are signs of current or
A. N. Sorokin   +5 more
doaj   +1 more source

Hydrophobic sequences target and anchor perilipin A to lipid droplets

open access: yesJournal of Lipid Research, 2004
Perilipins regulate triacylglycerol storage and hydrolysis in adipocytes. The central 25% of the perilipin A sequence, including three hydrophobic sequences (H1, H2, and H3) and an acidic region, targets and anchors perilipins to lipid droplets. Thus, we
Vidya Subramanian   +3 more
doaj   +1 more source

The Role of IKK-2/NF-κB Signaling Cascade in the Activation of Peritoneal Macrophages by Humic Acids of Peat

open access: yesРазработка и регистрация лекарственных средств, 2022
Introduction. Macrophages occupy a special place in the formation and coordination of the immune response, their population is characterized by heterogeneity, however, two main types are distinguished among them: pro-inflammatory (classically activated ...
E. S. Trofimova   +7 more
doaj   +1 more source

Optimising methods for the preservation, capture and identification of ubiquitin chains and ubiquitylated proteins by immunoblotting

open access: yesBiochemical and Biophysical Research Communications - BBRC, 2015
Immunoblotting is a powerful technique for the semi-quantitative analysis of ubiquitylation events, and remains the most commonly used method to study this process due to its high specificity, speed, sensitivity and relatively low cost.
Christoph H. Emmerich, P. Cohen
semanticscholar   +1 more source

Sexual activity in macropterous and brachypterous males of a flightless bug, Pyrrhocoris apterus (Heteroptera)

open access: yesEuropean Journal of Entomology, 2001
The long-winged (macropterous) and short-winged (brachypterous) adult males of Pyrrhocoris apterus (L.) from temperate (Czech Republic) and Mediterranean (Israel) populations were analysed for the sexual activity and the functional activity of their ...
Radomír SOCHA   +2 more
doaj   +1 more source

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